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          • Primary Antibodies ›
          • MYO1D Antibodies

          Invitrogen

          MYO1D Polyclonal Antibody

          View all (5) MYO1D antibodies

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          Cite MYO1D Polyclonal Antibody

          • Antibody Testing Data (1)
          MYO1D Antibody in Western Blot (WB)
          Group 53 Created with Sketch.
          MYO1D Antibody in Western Blot (WB)
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          FIGURE: 1 / 1

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          MYO1D Antibody (PA5-120743) in WB

          Western blot analysis of MYO1D in extracts of various cell lines. Samples were incubated with MYO1D Polyclonal antibody (Product # PA5-120743) using a dilution of 1:1,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s. {{ $ctrl.currentElement.advancedVerification.fullName }} validation info. View more
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          MYO1D Antibody in Western Blot (WB)

          Product Details

          PA5-120743

          Applications
          Tested Dilution
          Publications

          Western Blot (WB)

          1:500-1:1,000
          -

          ELISA (ELISA)

          1 µg/mL
          -
          Product Specifications

          Species Reactivity

          Human, Mouse

          Host/Isotype

          Rabbit / IgG

          Class

          Polyclonal

          Type

          Antibody

          Immunogen

          Recombinant fusion protein containing a sequence corresponding to amino acids 180-380 of human MYO1D (NP_001290209.1).
          View immunogen

          Conjugate

          Unconjugated Unconjugated Unconjugated

          Form

          Liquid

          Concentration

          1.276 mg/mL

          Purification

          Affinity Chromatography

          Storage buffer

          PBS, pH 7.3, with 50% glycerol

          Contains

          0.05% ProClin 300

          Storage conditions

          -20°C, Avoid Freeze/Thaw Cycles

          Shipping conditions

          Wet ice

          RRID

          AB_2914315

          Product Specific Information

          Positive test controls include: SKOV3, Mouse lung, Mouse brain.

          Immunogen sequence: HINNYLLEKS RVIVQQPGER SFHSFYQLLQ GGSEQMLRSL HLQKSLSSYN YIHVGAQLKS SINDAAEFRV VADAMKVIGF KPEEIQTVYK ILAAILHLGN LKFVVDGDTP LIENGKVVSI IAELLSTKTD MVEKALLYRT VATGRDIIDK QHTEQEASYG RDAFAKAIYE RLFCWIVTRI NDIIEVKNYD TTIHGKNTVI G

          Target Information

          Unconventional myosin that functions as actin-based motor protein with ATPase activity. Plays a role in endosomal protein trafficking, and especially in the transfer of cargo proteins from early to recycling endosomes. Required for normal planar cell polarity in ciliated tracheal cells, for normal rotational polarity of cilia, and for coordinated, unidirectional ciliary movement in the trachea. Required for normal, polarized cilia organization in brain ependymal epithelial cells.

          For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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          Cite this product

          Bioinformatics

          Protein Aliases: myo1d; myosin-I gamma; myosin-Id; protein phosphatase 1, regulatory subunit 108; Unconventional myosin-Id

          View more View less

          Gene Aliases: 9930104H07Rik; AW544947; D11Ertd9e; KIAA0727; MYO1D; myosin-1d; myr4; PPP1R108

          View more View less

          UniProt ID: (Human) O94832, (Mouse) Q5SYD0

          View more View less

          Entrez Gene ID: (Human) 4642, (Mouse) 338367

          View more View less

          Function(s)
          microfilament motor activity calmodulin binding ATP binding protein domain specific binding actin-dependent ATPase activity calcium-dependent protein binding actin filament binding nucleotide binding motor activity actin binding protein binding
          Process(es)
          metabolic process negative regulation of phosphatase activity cellular localization early endosome to recycling endosome transport
          It has to be done as per old AB suggested Products section.
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          Performance Guarantee

          If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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