Invitrogen

MYO7A Polyclonal Antibody

MYO7A Antibody in Immunocytochemistry (ICC/IF)

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MYO7A Antibody (PA1-936) in ICC/IF

Immunofluorescence analysis of MYO7A was performed using 70% confluent log phase L6 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with MYO7A Rabbit Polyclonal Antibody (Product # PA1-936) at 2 µg/mL in 0.1% BSA and incubated for 3 hours at room temperature and then labeled with Goat anti-Rabbit IgG (Heavy Chain) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A270... View More

MYO7A Antibody in Immunohistochemistry (PFA fixed) (IHC (PFA))

MYO7A Antibody in Immunohistochemistry (IHC)

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MYO7A Antibody — Antibody specificity was demonstrated by detection of differential basal expression of the target across tissue models owing to their inherent genetic constitution. Relative expression of MYO7A was observed in Mouse Skeletal Muscle, Rat Skeletal Muscle, Mouse Bone and Rat Heart in Western Blot using MYO7A Polyclonal Antibody (Product # PA1-936). MYO7A is reported to be expressed in skeletal tissues and not in other tissues like bone and heart. {RE}

Product Details

PA1-936

Applications

Tested Dilution

Publications

Western Blot (WB)

4 µg/mL

View 3 publications 3 publications

Immunohistochemistry (IHC)

View 10 publications 10 publications

Immunohistochemistry (Paraffin) (IHC (P))

View 1 publication 1 publication

Immunohistochemistry (PFA fixed) (IHC (PFA))

View 2 publications 2 publications

Immunocytochemistry (ICC/IF)

2 µg/mL

View 3 publications 3 publications

Product Specifications

Species Reactivity

Mouse, Rat

Published species

Mouse, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Synthetic Peptide: S(16) G Q E F D V P I G A V V K L C(31)

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS with 1mg/mL BSA

Contains

0.05% sodium azide

Storage conditions

-20°C, Avoid Freeze/Thaw Cycles

Shipping conditions

Ambient (domestic); Wet ice (international)

AB_2235704

Product Specific Information

PA1-936 detects myosin VIIa from mouse tissues as well as recombinant protein.

PA1-936 has been successfully used in Western blot procedures. By Western blot, this antibody detects an ~220 kDa protein representing myosin VIIa from mouse testes preparations. PA1-936 detects recombinant mouse myosin VIIa overexpressed in Sf9 insect cell lysate.

PA1-936 immunizing peptide corresponds to amino acid residues 16-31 from mouse myosin VIIa. This sequence is completely conserved in mouse, porcine, and human myosin VIIa. PA1-936 immunizing peptide (Cat. # PEP-045) is available for use in neutralization and control experiments.

Target Information

Myosin VIIa is a member of the myosin superfamily of actin-based motor proteins. Defects in the myosin VIIa gene are responsible for hearing impairment in shaker-1 (sh1) mice and causes Usher syndrome IB in humans. Usher syndrome associates congenital deafness, vestibular dysfunction, and retinitis pigmentosa and is the most common form of combined deafness and blindness. Structural features of myosin VIIa protein include an ATP binding N-terminal motor domain, a central region which possess five light-chain binding (IQ) motifs, and a C-terminal domain with three myosin tail homology (MyTH4) and talin-like homology regions.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Immunohistochemistry localises myosin-7a to cochlear efferent boutons.

Wellcome open research

Sirko P,Kozlov AS

PA1-936 was used in Immunohistochemistry to show that in addition to its role in stereocilia formation and maintenance, myosin 7a or certain myosin-7a expression variants might play a role in efferent synaptic transmission in the cochlea and thus ultimately influence cochlear gain regulation.

Sat Aug 24 00:00:00 UTC 2024

Immunohistochemistry localises myosin-7a to cochlear efferent boutons.

Wellcome open research

Sirko P,Kozlov AS

Sat Aug 24 00:00:00 UTC 2024

Tsukushi is essential for the formation of the posterior semicircular canal that detects gait performance.

Journal of cell communication and signaling

Miwa T,Ito N,Ohta K

PA1-936 was used in Immunohistochemistry to investigate the function of Tsukushi in the vestibules.

Wed Dec 01 00:00:00 UTC 2021

Disruption of Hars2 in Cochlear Hair Cells Causes Progressive Mitochondrial Dysfunction and Hearing Loss in Mice.

Frontiers in cellular neuroscience

Xu P,Wang L,Peng H,Liu H,Liu H,Yuan Q,Lin Y,Xu J,Pang X,Wu H,Yang T

PA1-936 was used in Immunohistochemistry (PFA) to suggest that conditional knockout of Hars2 in mouse cochlear hair cells leads to accumulating mitochondrial dysfunction and ROS stress, triggers progressive hearing loss highlighted by hair cell synaptopathy and apoptosis, and is differentially perceived by inner and outer hair cells.

Tue Jan 04 00:00:00 UTC 2022

Disruption of Hars2 in Cochlear Hair Cells Causes Progressive Mitochondrial Dysfunction and Hearing Loss in Mice.

Frontiers in cellular neuroscience

Xu P,Wang L,Peng H,Liu H,Liu H,Yuan Q,Lin Y,Xu J,Pang X,Wu H,Yang T

Tue Jan 04 00:00:00 UTC 2022

Coxsackievirus B4 Transplacental Infection Severely Disturbs Central Tolerogenic Mechanisms in the Fetal Thymus.

Microorganisms

Halouani A,Michaux H,Jmii H,Trussart C,Chahbi A,Martens H,Renard C,Aouni M,Hober D,Geenen V,Jaïdane H

Published figure using MYO7A polyclonal antibody (Product # PA1-936) in Western Blot

Mon Jul 19 00:00:00 UTC 2021

Tsukushi is essential for the development of the inner ear.

Molecular brain

Miwa T,Ohta K,Ito N,Hattori S,Miyakawa T,Takeo T,Nakagata N,Song WJ,Minoda R

PA1-936 was used in Immunocytochemistry to reveal that TSK interacted with Sox2 and BMP4 to control stereocilia formation in the inner hair cells.

Tue Mar 03 00:00:00 UTC 2020

Tsukushi is essential for the development of the inner ear.

Molecular brain

Miwa T,Ohta K,Ito N,Hattori S,Miyakawa T,Takeo T,Nakagata N,Song WJ,Minoda R

PA1-936 was used in Immunocytochemistry to reveal that TSK interacted with Sox2 and BMP4 to control stereocilia formation in the inner hair cells.

Tue Mar 03 00:00:00 UTC 2020

Disruption of Atg7-dependent autophagy causes electromotility disturbances, outer hair cell loss, and deafness in mice.

Cell death & disease

Zhou H,Qian X,Xu N,Zhang S,Zhu G,Zhang Y,Liu D,Cheng C,Zhu X,Liu Y,Lu L,Tang J,Chai R,Gao X

PA1-936 was used in Immunohistochemistry to show that OHCs orchestrate intricate nonselective and selective autophagic/mitophagy pathways working in concert to maintain cellular homeostasis.

Sat Oct 24 00:00:00 UTC 2020

The expression of NLRX1 in C57BL/6 mice cochlear hair cells: Possible relation to aging- and neomycin-induced deafness.

Neuroscience letters

Yang Q,Sun G,Cao Z,Yin H,Qi Q,Wang J,Liu W,Bai X,Wang H,Li J

PA1-936 was used in immunocytochemistry to study the relationship between aging and neomycin-induced deafness due to expression of NLRX1 in C57BL/6 mice cochlear hair cells

Fri Mar 11 00:00:00 UTC 2016

Expression of prestin in OHCs is reduced in Spag6 gene knockout mice.

Neuroscience letters

Wang J,Li X,Zhang Z,Wang H,Li J

PA1-936 was used in western blot to determine the expression of SPAG6 in cochlear hair cells and its influence on prestin

Fri Apr 10 00:00:00 UTC 2015

Notch signaling alters sensory or neuronal cell fate specification of inner ear stem cells.

The Journal of neuroscience : the official journal of the Society for Neuroscience

Jeon SJ,Fujioka M,Kim SC,Edge AS

PA1-936 was used in immunohistochemistry to investigate the role of Notch pathway in the fate determination of inner ear stem cells

Wed Jun 08 00:00:00 UTC 2011

MicroRNA-183 family expression in hair cell development and requirement of microRNAs for hair cell maintenance and survival.

Developmental dynamics : an official publication of the American Association of Anatomists

Weston MD,Pierce ML,Jensen-Smith HC,Fritzsch B,Rocha-Sanchez S,Beisel KW,Soukup GA

PA1-936 was used in immunohistochemistry to study the expression of microRNAs in the hair cells of the mouse inner ear

Fri Apr 01 00:00:00 UTC 2011

Expression of candidate markers for stem/progenitor cells in the inner ears of developing and adult GFAP and nestin promoter-GFP transgenic mice.

Gene expression patterns : GEP

Smeti I,Savary E,Capelle V,Hugnot JP,Uziel A,Zine A

PA1-936 was used in immunohistochemistry to study the mechanism for permanent sensori-neural hearing loss

Thu Mar 24 00:00:00 UTC 2011

Phosphatase and tensin homolog deleted on chromosome 10 regulates sensory cell proliferation and differentiation of hair bundles in the mammalian cochlea.

Neuroscience

Dong Y,Sui L,Yamaguchi F,Kamitori K,Hirata Y,Hossain MA,Suzuki A,Holley MC,Tokuda M

PA1-936 was used in immunohistochemistry to investigate the effect of PTEN on auditory sensory epithelial cells

Wed Nov 10 00:00:00 UTC 2010

Role of phosphatase and tensin homolog in the development of the mammalian auditory system.

Neuroreport

Dong Y,Sui L,Yamaguchi F,Kamitori K,Hirata Y,Suzuki A,Holley M,Tokuda M

PA1-936 was used in immunohistochemistry to investigate the involvement of PTEN in the development of mammalian auditory system

Wed Jul 14 00:00:00 UTC 2010

Retention of progenitor cell phenotype in otospheres from guinea pig and mouse cochlea.

Journal of translational medicine

Oiticica J,Barboza-Junior LC,Batissoco AC,Lezirovitz K,Mingroni-Netto RC,Haddad LA,Bento RF

PA1-936 was used in immunocytochemistry to investigate the progenitor cell phenotype in otosphere suspension cultures of dissociated organ of Corti from neonatal mouse and guinea pig

Thu Nov 18 00:00:00 UTC 2010

A missense mutation in the previously undescribed gene Tmhs underlies deafness in hurry-scurry (hscy) mice.

Proceedings of the National Academy of Sciences of the United States of America

Longo-Guess CM,Gagnon LH,Cook SA,Wu J,Zheng QY,Johnson KR

PA1-936 was used in immunohistochemistry to identify a novel gene Tmhs related to the formation of hair cell stereocilia.

Tue May 31 00:00:00 UTC 2005

Myosin VIIa participates in opsin transport through the photoreceptor cilium.

The Journal of neuroscience : the official journal of the Society for Neuroscience

Liu X,Udovichenko IP,Brown SD,Steel KP,Williams DS

PA1-936 was used in western blot to demonstrate that myosin VIIa function in opsin transport through the photoreceptor cilium

Sun Aug 01 00:00:00 UTC 1999

Bioinformatics

Protein Aliases: motor protein; MyO VIIa; myosin 7a; myosin VIIA (Usher syndrome 1B (autosomal recessive, severe)); myosin-VIIa; shaker 1; Unconventional myosin-VIIa

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Gene Aliases: Hdb; Myo7; Myo7a; nmf371; polka; sh-1; sh1; USH1B

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UniProt ID: (Mouse) P97479

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Entrez Gene ID: (Mouse) 17921, (Rat) 266714

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Function(s)

microfilament motor activity nucleotide binding motor activity actin binding protein binding calmodulin binding ATP binding protein domain specific binding spectrin binding actin-dependent ATPase activity protein complex binding protein homodimerization activity ADP binding protein N-terminus binding actin filament binding

Process(es)

phagolysosome assembly transport intracellular protein transport phagocytosis lysosome organization sensory perception visual perception sensory perception of sound metabolic process cell projection organization actin filament-based movement inner ear morphogenesis mechanoreceptor differentiation auditory receptor cell differentiation post-embryonic organ morphogenesis inner ear development sensory perception of light stimulus equilibrioception pigment granule localization pigment granule transport auditory receptor cell stereocilium organization inner ear receptor cell differentiation inner ear receptor stereocilium organization

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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MYO7A Polyclonal Antibody

MYO7A Antibody (PA1-936) in ICC/IF

Product Details

PA1-936

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (Paraffin) (IHC (P))

Immunohistochemistry (PFA fixed) (IHC (PFA))

Immunocytochemistry (ICC/IF)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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