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MZF1 Antibody (PA5-120086) in WB
Western blot analysis of MZF1 in extracts of various cell lines. Samples were incubated with MZF1 Polyclonal antibody (Product # PA5-120086) using a dilution of 1:1,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3s.
Product Details
PA5-120086
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 100-350 of human MZF1 (NP_003413.2).
Conjugate
Form
Liquid
Concentration
2.36 mg/mL
Purification
Affinity Chromatography
Storage buffer
PBS, pH 7.3, with 50% glycerol
Contains
0.01% thimerosal
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2913658
Product Specific Information
Positive test controls include: HeLa, A-431, U-251MG, 293T, SGC-7901, Mouse brain, Mouse lung, Rat brain. The target is usually found in the following locations: Nucleus.
Immunogen sequence: IQARVQGQRP GSPEEAAALV DGLRREPGGP RRWVTVQVQG QEVLSEKMEP SSFQPLPETE PPTPEPGPKT PPRTMQESPL GLQVKEESEV TEDSDFLESG PLAATQESVP TLLPEEAQRC GTVLDQIFPH SKTGPEGPSW REHPRALWHE EAGGIFSPGF ALQLGSISAG PGSVSPHLHV PWDLGMAGLS GQIQSPSREG GFAHALLLPS DLRSEQDPTD EDPCRGVGPA LITTRWRSPR GRSRGRPSTG G
Target Information
Binds to target promoter DNA and functions as transcription regulator. Regulates transcription from the PADI1 and CDH2 promoter. May be one regulator of transcriptional events during hemopoietic development. [UniProt]
⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Myeloid zinc finger 1; Myeloid zinc finger protein 1; Myeloid zinc finger protein 2; myeloid-specific retinoic acid-responsive zinc finger protein; MZF-1; Zinc finger and SCAN domain-containing protein 6; zinc finger protein 121; Zinc finger protein 42; zinc finger protein 42 (myeloid-specific retinoic acid-responsive); zinc finger protein 98
Gene Aliases: MZF; MZF-1; Mzf-2; MZF1; MZF1B; Mzf2; Zfp121; ZFP98; ZNF42; ZSCAN6
UniProt ID: (Human) P28698
Entrez Gene ID: (Human) 7593, (Mouse) 109889, (Rat) 361508
RNA polymerase II core promoter proximal region sequence-specific DNA binding
transcriptional activator activity, RNA polymerase II core promoter proximal region sequence-specific binding
transcriptional repressor activity, RNA polymerase II core promoter proximal region sequence-specific binding
transcription factor activity, sequence-specific DNA binding
protein binding
protein homodimerization activity
transcription regulatory region DNA binding
metal ion binding
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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