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Invitrogen
Marginal Zone B Cells Monoclonal Antibody (HIS57), eBioscience™
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Marginal Zone B Cells Antibody (14-9489-82) in IHC (F)
Immunohistochemistry of frozen rat spleen using 5 µg/mL of Anti-Rat Marginal Zone B Cells Purified, followed by F (ab')2 Anti-Mouse IgG eFluor® 660 (white), Anti-Rat CD3 Biotin, followed by Streptavidin eFluor® 570 (red), and Anti-Rat CD45R (B220) FITC (green).Nuclei are stained with DAPI (blue).
Product Details
14-9489-82
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Rat
Host/Isotype
Mouse
/ IgG1, kappa
Class
Monoclonal
Type
Antibody
Clone
HIS57
Conjugate
Form
Liquid
Concentration
0.5 mg/mL
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.2
Contains
0.09% sodium azide
Storage conditions
4°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2572947
Product Specific Information
Description: The monoclonal antibody HIS57 recognizes an unknown antigen expressed by rat marginal zone B cells. Marginal zone B cells are an anatomical B cell compartment located in the spleen at the area between red and white pulp. Marginal zone B cells serve as a first line of defense against blood-borne antigens in a T cell-independent manner. Marginal zone B cells can also be phenotyped as B cells that are IgM high, IgD low, CD21 high and CD23 low.
Applications Reported: This HIS57 antibody has been reported for use in flow cytometric analysis, and immunohistochemical staining of frozen tissue sections.
Applications Tested: This HIS57 antibody has been tested by immunohistochemistry of frozen rat tissue and can be used at less than or equal to 5 µg/mL. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
Purity: Greater than 90%, as determined by SDS-PAGE.
Aggregation: Less than 10%, as determined by HPLC.
Filtration: 0.2 µm post-manufacturing filtered.
Target Information
Marginal zone B cells are an anatomical B cell compartment located in the spleen at the area between red and white pulp. Marginal zone B cells serve as a first line of defense against blood-borne antigens in a T cell-independent manner. Marginal zone B cells can also be phenotyped as B cells that are IgM high, IgD low, CD21 high and CD23 low.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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