Invitrogen

Mature Macrophage Marker Monoclonal Antibody (HIS36), PE, eBioscience™

Mature Macrophage Marker Antibody in Flow Cytometry (Flow)

FIGURE: 1 / 1

Mature Macrophage Marker Antibody (12-0660-82) in Flow

Staining of rat thioglycolate-induced peritoneal exudate cells (PECs) with 0.25 µg of Mouse IgG2a kappa Isotype Control PE (Product # 12-4724-81) (open histogram) and 0.25 µg of Anti-Rat Macrophage Marker PE (filled histogram). Total viable cells were used for analysis.

Product Image Gallery

Product Details

12-0660-82

Applications

Tested Dilution

Publications

Flow Cytometry (Flow)

0.5 µg/test

View 6 publications 6 publications

Product Specifications

Species Reactivity

Rat

Published species

Rat

Host/Isotype

Mouse / IgG2a, kappa

Recommended Isotype Control

Mouse IgG2a kappa Isotype Control (eBM2a), PE, eBioscience™

Class

Monoclonal

Type

Antibody

Clone

HIS36

Conjugate

PE PE PE

Excitation/Emission Max

565/576 nm View spectra

Form

Liquid

Concentration

0.2 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2

Contains

0.09% sodium azide

Storage conditions

4°C, store in dark, DO NOT FREEZE!

Shipping conditions

Ambient (domestic); Wet ice (international)

AB_465730

Product Specific Information

Description: The HIS36 monoclonal antibody reacts with a molecule expressed on the surface of rat macrophages. The antigen recognized by HIS36 is an ED2-like antigen expressed by thioglycolate-elicited peritoneal cells and tissue macrophages. This antibody is used as a marker for rat tissue macrophages.

Applications Reported: The HIS36 antibody has been reported for use in flow cytometric analysis.

Applications Tested: The HIS36 antibody has been tested by flow cytometric analysis of rat spleen and peritoneal cells. This can be used at less than or equal to 0.5 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Excitation: 488-561 nm; Emission: 578 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.

Filtration: 0.2 µm post-manufacturing filtered.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

How to use the Panel Builder

Watch the video to learn how to use the Invitrogen Flow Cytometry Panel Builder to build your next flow cytometry panel in 5 easy steps.

Product References

Dusp6 deficiency attenuates neutrophil-mediated cardiac damage in the acute inflammatory phase of myocardial infarction.

Nature communications

Zhou X,Zhang C,Wu X,Hu X,Zhang Y,Wang X,Zheng L,Gao P,Du J,Zheng W,Shang H,Hu K,Jiang Z,Nie Y,Hu S,Xiao RP,Zhu X,Xiong JW

12-0660-82 was used in Flow cytometry/Cell sorting to provide robust animal models and novel insights for neutrophil-mediated cardiac damage and demonstrate the potential of DUSP6 as a therapeutic target for post-MI cardiac remodeling and other relevant inflammatory diseases.

Sat Nov 05 00:00:00 UTC 2022

Prenatal alcohol-induced sex differences in immune, metabolic and neurobehavioral outcomes in adult rats.

Brain, behavior, and immunity

Bake S,Pinson MR,Pandey S,Chambers JP,Mota R,Fairchild AE,Miranda RC,Sohrabji F

12-0660-82 was used in Flow cytometry/Cell sorting to show that peripheral immune status is a persistent, sex-dependent predictor of glucose intolerance and neurobehavioral function in adult PAE offspring.

Mon Nov 01 00:00:00 UTC 2021

MiRNA-210 induces microglial activation and regulates microglia-mediated neuroinflammation in neonatal hypoxic-ischemic encephalopathy.

Cellular & molecular immunology

Li B,Dasgupta C,Huang L,Meng X,Zhang L

12-0660-82 was used in Flow Cytometry to report in an animal model of neonatal rats that HIE significantly upregulated miR-210 expression in microglia in the neonatal brain and strongly induced activated microglia.

Tue Sep 01 00:00:00 UTC 2020

Gene Network Analysis of Interstitial Macrophages After Treatment with Induced Pluripotent Stem Cells Secretome (iPSC-cm) in the Bleomycin Injured Rat Lung.

Stem cell reviews and reports

Tamò L,Simillion C,Hibaoui Y,Feki A,Gugger M,Prasse A,Jäger B,Goldmann T,Geiser T,Gazdhar A

12-0660 was used in Flow cytometry/Cell sorting to demonstrate that iPSC-cm reduces fibrosis in bleomycin injured rat lung by partially altering the macrophages and regulating their gene expression.

Fri Jun 01 00:00:00 UTC 2018

Adenoviral transduction of mesenchymal stem cells: in vitro responses and in vivo immune responses after cell transplantation.

PloS one

Treacy O,Ryan AE,Heinzl T,O'Flynn L,Cregg M,Wilk M,Odoardi F,Lohan P,O'Brien T,Nosov M,Ritter T

12-0660 was used in Flow cytometry/Cell sorting to investigate whether adenovirus-modification of mesenchymal stem cells alters their immunological properties in vitro and in vivo.

Thu Jan 10 00:00:00 UTC 2013

Aging per se increases the susceptibility to free fatty acid-induced insulin resistance.

The journals of gerontology. Series A, Biological sciences and medical sciences

Einstein FH,Huffman DM,Fishman S,Jerschow E,Heo HJ,Atzmon G,Schechter C,Barzilai N,Muzumdar RH

12-0660 was used in Flow cytometry/Cell sorting to determine the effects of an acute elevation in FFA on insulin action with aging.

Sun Aug 01 00:00:00 UTC 2010

Bioinformatics

Protein Aliases: ED2-like antigen; Macrophages; Mature Macrophages

View more View less

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

Learn more