Proteintech

Mecr Polyclonal Antibody

Mecr Antibody in Immunocytochemistry (ICC/IF)

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Mecr Antibody (51027-2-AP) in ICC/IF

Immunofluorescent analysis of (4% PFA) fixed C2C12 cells using Mecr antibody (Product # 51027-2-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

Mecr Antibody in Immunohistochemistry (Paraffin) (IHC (P))

Mecr Antibody in Immunoprecipitation (IP)

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Product Details

51027-2-AP

Applications

Tested Dilution

Publications

Western Blot (WB)

1:1,000-1:4,000

View 4 publications 4 publications

Immunohistochemistry (IHC)

View 1 publication 1 publication

Immunohistochemistry (Paraffin) (IHC (P))

1:20-1:200

Immunocytochemistry (ICC/IF)

1:200

View 1 publication 1 publication

Immunoprecipitation (IP)

1:1,500

Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Human, Mouse

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Mecr Fusion Protein Ag0465 (117-373 aa encoded by BC003864)

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.17 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C

Shipping conditions

Wet ice (domestic); Dry ice (international)

Product Specific Information

Immunogen sequence: SSVSALKPG DWVIPANAGL GTWRTEAVFS EEALIGIPKD IPLQSAATLG VNPCTAYRML VDFEQLQPGD SVIQNASNSG VGQAVIQIAS ALRLKTINVV RDRPDIKKLT DRLKDLGADY VLTEEELRMP ETKTIFKDLP LPRLALNCVG GKSSTELLRH LAPGGTMVTY GGMAKQPVTA SVSLLIFKDL KLRGFWLSQW KKNHSPDEFK ELILTLCNLI RQGRLTAPSC SEVPLQGYQQ ALEASMKPFV SSKQILTM (117-373 aa encoded by BC003864)

Target Information

The mitochondrial trans-2-enoyl-CoA reductase (MECR), was initially identified as nuclear receptor-binding factor 1 (NRBF1), which can interact with a multitude of nuclear hormone receptors in the presence of the respective ligands. MECR has been shown to be part of the mitochondrial fatty acid synthesis (FAS II) system and to catalyze the NAPDH-dependent reduction of 2-enoyl thioesters, generating saturated acyl-groups. Overexpression of this gene in transgenic mice can lead to cardiac abnormalities, suggesting that inappropriate expression of genes of FAS II can result in the development of hereditary cardiomyopathy.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

An engineered variant of MECR reductase reveals indispensability of long-chain acyl-ACPs for mitochondrial respiration.

Nature communications

Rahman MT,Koski MK,Panecka-Hofman J,Schmitz W,Kastaniotis AJ,Wade RC,Wierenga RK,Hiltunen JK,Autio KJ

Sat Feb 04 00:00:00 UTC 2023

Mitochondrial fatty acid synthesis coordinates oxidative metabolism in mammalian mitochondria.

eLife

Nowinski SM,Solmonson A,Rusin SF,Maschek JA,Bensard CL,Fogarty S,Jeong MY,Lettlova S,Berg JA,Morgan JT,Ouyang Y,Naylor BC,Paulo JA,Funai K,Cox JE,Gygi SP,Winge DR,DeBerardinis RJ,Rutter J

Mon Aug 17 00:00:00 UTC 2020

Impaired Mitochondrial Fatty Acid Synthesis Leads to Neurodegeneration in Mice.

The Journal of neuroscience : the official journal of the Society for Neuroscience

Nair RR,Koivisto H,Jokivarsi K,Miinalainen IJ,Autio KJ,Manninen A,Poutiainen P,Tanila H,Hiltunen JK,Kastaniotis AJ

Wed Nov 07 00:00:00 UTC 2018

Impaired Mitochondrial Fatty Acid Synthesis Leads to Neurodegeneration in Mice.

The Journal of neuroscience : the official journal of the Society for Neuroscience

Nair RR,Koivisto H,Jokivarsi K,Miinalainen IJ,Autio KJ,Manninen A,Poutiainen P,Tanila H,Hiltunen JK,Kastaniotis AJ

Wed Nov 07 00:00:00 UTC 2018

Impaired Mitochondrial Fatty Acid Synthesis Leads to Neurodegeneration in Mice.

The Journal of neuroscience : the official journal of the Society for Neuroscience

Nair RR,Koivisto H,Jokivarsi K,Miinalainen IJ,Autio KJ,Manninen A,Poutiainen P,Tanila H,Hiltunen JK,Kastaniotis AJ

Wed Nov 07 00:00:00 UTC 2018

Genetic modifications of Mecr reveal a role for mitochondrial 2-enoyl-CoA/ACP reductase in placental development in mice.

Human molecular genetics

Nair RR,Kerätär JM,Autio KJ,Masud AJ,Finnilä MAJ,Autio-Harmainen HI,Miinalainen IJ,Nieminen PA,Hiltunen JK,Kastaniotis AJ

Thu Jun 01 00:00:00 UTC 2017

MECR Mutations Cause Childhood-Onset Dystonia and Optic Atrophy, a Mitochondrial Fatty Acid Synthesis Disorder.

American journal of human genetics

Heimer G,Kerätär JM,Riley LG,Balasubramaniam S,Eyal E,Pietikäinen LP,Hiltunen JK,Marek-Yagel D,Hamada J,Gregory A,Rogers C,Hogarth P,Nance MA,Shalva N,Veber A,Tzadok M,Nissenkorn A,Tonduti D,Renaldo F,Kraoua I,Panteghini C,Valletta L,Garavaglia B,Cowley MJ,Gayevskiy V,Roscioli T,Silberstein JM,Hoffmann C,Raas-Rothschild A,Tiranti V,Anikster Y,Christodoulou J,Kastaniotis AJ,Ben-Zeev B,Hayflick SJ

Thu Dec 01 00:00:00 UTC 2016

MECR Mutations Cause Childhood-Onset Dystonia and Optic Atrophy, a Mitochondrial Fatty Acid Synthesis Disorder.

American journal of human genetics

Thu Dec 01 00:00:00 UTC 2016

Bioinformatics

Protein Aliases: 2-enoyl thioester reductase; Enoyl-[acyl-carrier-protein] reductase, mitochondrial; homolog of yeast 2-enoyl thioester reductase; HsNrbf-1; mitochondrial 2-enoyl thioester reductase; NRBF-1; nuclear receptor binding factor 1; Nuclear receptor-binding factor 1; trans-2-enoyl-CoA reductase, mitochondrial

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Gene Aliases: AI195831; CGI-63; FASN2B; MECR; NBRF1; NRBF1

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UniProt ID: (Human) Q9BV79, (Mouse) Q9DCS3, (Rat) Q9Z311

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Entrez Gene ID: (Human) 51102, (Mouse) 26922, (Rat) 29470

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Function(s)

zinc ion binding trans-2-enoyl-CoA reductase (NADPH) activity receptor binding oxidoreductase activity ligand-dependent nuclear receptor binding

Process(es)

lipid metabolic process fatty acid metabolic process fatty acid biosynthetic process oxidation-reduction process

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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Mecr Polyclonal Antibody

Mecr Antibody (51027-2-AP) in ICC/IF

Product Details

51027-2-AP

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (Paraffin) (IHC (P))

Immunocytochemistry (ICC/IF)

Immunoprecipitation (IP)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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