Proteintech

Melanophilin Polyclonal Antibody

Melanophilin Antibody in Immunocytochemistry (ICC/IF)

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Melanophilin Antibody (10338-1-AP) in ICC/IF

Immunofluorescent analysis of ( -20°C Ethanol ) fixed HeLa cells using 10338-1-AP (Melanophilin antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IGG (H+L).

Melanophilin Antibody in Immunohistochemistry (Paraffin) (IHC (P))

Melanophilin Antibody in Western Blot (WB)

Melanophilin Antibody in Immunoprecipitation (IP)

Product Image Gallery

Product Details

10338-1-AP

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:1,000

View 14 publications 14 publications

Immunohistochemistry (IHC)

View 1 publication 1 publication

Immunohistochemistry (Paraffin) (IHC (P))

1:50-1:500

Immunocytochemistry (ICC/IF)

1:20-1:200

View 2 publications 2 publications

Immunoprecipitation (IP)

0.5-4.0 µg

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human

Published species

Human

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Melanophilin Fusion Protein Ag0316 (8-236 aa encoded by BC001653)

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.37 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C

Shipping conditions

Wet ice (domestic); Dry ice (international)

Product Specific Information

Immunogen sequence: SKLTDEEAQ HVLEVVQRDF DLRRKEEERL EALKGKIKKE SSKRELLSDT AHLNETHCAR CLQPYQLLVN SKRQCLECGL FTCKSCGRVH PEEQGWICDP CHLARVVKIG SLEWYYEHVK ARFKRFGSAK VIRSLHGRLQ GGAGPELISE ERSGDSDQTD EDGEPGSEAQ AQAQPFGSKK KRLLSVHDFD FEGDSDDSTQ PQGHSLHLSS VPEARDSPQS LTDESCSEKA (8-236 aa encoded by BC001653)

Target Information

Slac2-a (for synaptotagmin-like protein (Slp) homolog lacking C2 domains-a) links Rab27A on melanosomes with Myosin VA in melanocytes. Slac2-a binds Myosin VA through a C-terminal region and GTP-bound Rab27A through its synaptotagmin-like protein homology domain (SHD), which is located near the N-terminus. The transport of pigment and cytotoxic granules in melanocytes requires the stable formation of this complex, and thus mutations in the binding domains of the three protein components may cause albinism and/or severe immune disorders.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Phosphorylation of glucocorticoid receptor induced by 16-kauren-2-beta-18, 19-triol decreases expression of Melanophilin through JNK signalling.

Experimental dermatology

Myung CH,Jo CS,Hwang JS

Fri Sep 01 00:00:00 UTC 2023

Phosphorylation of glucocorticoid receptor induced by 16-kauren-2-beta-18, 19-triol decreases expression of Melanophilin through JNK signalling.

Experimental dermatology

Myung CH,Jo CS,Hwang JS

Fri Sep 01 00:00:00 UTC 2023

Functional hierarchy among different Rab27 effectors involved in secretory granule exocytosis.

eLife

Zhao K,Matsunaga K,Mizuno K,Wang H,Okunishi K,Izumi T

Tue Feb 21 00:00:00 UTC 2023

Identification of a Novel MLPH Missense Mutation in a Chinese Griscelli Syndrome 3 Patient.

Frontiers in medicine

Huang Q,Yuan Y,Gong J,Zhang T,Qi Z,Yang X,Li W,Wei A

Tue May 24 00:00:00 UTC 2022

Coal dust exposure triggers heterogeneity of transcriptional profiles in mouse pneumoconiosis and Vitamin D remedies.

Particle and fibre toxicology

Mu M,Li B,Zou Y,Wang W,Cao H,Zhang Y,Sun Q,Chen H,Ge D,Tao H,Hu D,Yuan L,Tao X,Wang J

Thu Jan 20 00:00:00 UTC 2022

Coal dust exposure triggers heterogeneity of transcriptional profiles in mouse pneumoconiosis and Vitamin D remedies.

Particle and fibre toxicology

Mu M,Li B,Zou Y,Wang W,Cao H,Zhang Y,Sun Q,Chen H,Ge D,Tao H,Hu D,Yuan L,Tao X,Wang J

Thu Jan 20 00:00:00 UTC 2022

Regulation of Melanophilin (Mlph) gene expression by the glucocorticoid receptor (GR).

Scientific reports

Myung CH,Lee JE,Jo CS,Park JI,Hwang JS

Thu Aug 19 00:00:00 UTC 2021

A novel function of Prohibitin on melanosome transport in melanocytes.

Theranostics

Jo CS,Park HI,Jung HJ,Park JI,Lee JE,Myung CH,Hwang JS

Tue May 18 00:00:00 UTC 2021

MLPH Accelerates the Epithelial-Mesenchymal Transition in Prostate Cancer.

OncoTargets and therapy

Zhang T,Sun Y,Zheng T,Wang R,Jia D,Zhang W

Wed Dec 20 00:00:00 UTC 2023

16-Kauren-2-beta-18,19-triol inhibits melanosome transport in melanocytes by down-regulation of melanophilin expression.

Journal of dermatological science

Myung CH,Kim K,Park JI,Lee JE,Lee JA,Hong SC,Lim KM,Hwang JS

Sat Feb 01 00:00:00 UTC 2020

Identification of MicroRNA Targeting Mlph and Affecting Melanosome Transport.

Biomolecules

Lee JA,Hwang SJ,Hong SC,Myung CH,Lee JE,Park JI,Hwang JS

Mon Jul 08 00:00:00 UTC 2019

Inhibitory effect of 2-methyl-naphtho[1,2,3-de]quinolin-8-one on melanosome transport and skin pigmentation.

Scientific reports

Park JI,Lee HY,Lee JE,Myung CH,Hwang JS

Wed Jul 06 00:00:00 UTC 2016

Inhibitory effect of 2-methyl-naphtho[1,2,3-de]quinolin-8-one on melanosome transport and skin pigmentation.

Scientific reports

Park JI,Lee HY,Lee JE,Myung CH,Hwang JS

Wed Jul 06 00:00:00 UTC 2016

Patients with Griscelli syndrome and normal pigmentation identify RAB27A mutations that selectively disrupt MUNC13-4 binding.

The Journal of allergy and clinical immunology

Cetica V,Hackmann Y,Grieve S,Sieni E,Ciambotti B,Coniglio ML,Pende D,Gilmour K,Romagnoli P,Griffiths GM,Aricò M

Fri May 01 00:00:00 UTC 2015

Patients with Griscelli syndrome and normal pigmentation identify RAB27A mutations that selectively disrupt MUNC13-4 binding.

The Journal of allergy and clinical immunology

Cetica V,Hackmann Y,Grieve S,Sieni E,Ciambotti B,Coniglio ML,Pende D,Gilmour K,Romagnoli P,Griffiths GM,Aricò M

Fri May 01 00:00:00 UTC 2015

Patients with Griscelli syndrome and normal pigmentation identify RAB27A mutations that selectively disrupt MUNC13-4 binding.

The Journal of allergy and clinical immunology

Cetica V,Hackmann Y,Grieve S,Sieni E,Ciambotti B,Coniglio ML,Pende D,Gilmour K,Romagnoli P,Griffiths GM,Aricò M

Fri May 01 00:00:00 UTC 2015

Patients with Griscelli syndrome and normal pigmentation identify RAB27A mutations that selectively disrupt MUNC13-4 binding.

The Journal of allergy and clinical immunology

Cetica V,Hackmann Y,Grieve S,Sieni E,Ciambotti B,Coniglio ML,Pende D,Gilmour K,Romagnoli P,Griffiths GM,Aricò M

Fri May 01 00:00:00 UTC 2015

Cellular and clinical report of new Griscelli syndrome type III cases.

Pigment cell & melanoma research

Westbroek W,Klar A,Cullinane AR,Ziegler SG,Hurvitz H,Ganem A,Wilson K,Dorward H,Huizing M,Tamimi H,Vainshtein I,Berkun Y,Lavie M,Gahl WA,Anikster Y

Sun Jan 01 00:00:00 UTC 2012

Cellular and clinical report of new Griscelli syndrome type III cases.

Pigment cell & melanoma research

Westbroek W,Klar A,Cullinane AR,Ziegler SG,Hurvitz H,Ganem A,Wilson K,Dorward H,Huizing M,Tamimi H,Vainshtein I,Berkun Y,Lavie M,Gahl WA,Anikster Y

Sun Jan 01 00:00:00 UTC 2012

Differential gene expression in normal esophagus and Barrett's esophagus.

Journal of gastroenterology

Wang J,Qin R,Ma Y,Wu H,Peters H,Tyska M,Shaheen NJ,Chen X

Tue Jan 19 00:00:00 UTC 2010

Differential gene expression in normal esophagus and Barrett's esophagus.

Journal of gastroenterology

Wang J,Qin R,Ma Y,Wu H,Peters H,Tyska M,Shaheen NJ,Chen X

Tue Jan 19 00:00:00 UTC 2010

Knockdown of myosin Va isoforms by RNAi as a tool to block melanosome transport in primary human melanocytes.

The Journal of investigative dermatology

Van Gele M,Geusens B,Schmitt AM,Aguilar L,Lambert J

Wed Oct 01 00:00:00 UTC 2008

Knockdown of myosin Va isoforms by RNAi as a tool to block melanosome transport in primary human melanocytes.

The Journal of investigative dermatology

Van Gele M,Geusens B,Schmitt AM,Aguilar L,Lambert J

Wed Oct 01 00:00:00 UTC 2008

A novel missense mutation (G43S) in the switch I region of Rab27A causing Griscelli syndrome.

Molecular genetics and metabolism

Westbroek W,Tuchman M,Tinloy B,De Wever O,Vilboux T,Hertz JM,Hasle H,Heilmann C,Helip-Wooley A,Kleta R,Gahl WA

Sun Jun 01 00:00:00 UTC 2008

A novel missense mutation (G43S) in the switch I region of Rab27A causing Griscelli syndrome.

Molecular genetics and metabolism

Westbroek W,Tuchman M,Tinloy B,De Wever O,Vilboux T,Hertz JM,Hasle H,Heilmann C,Helip-Wooley A,Kleta R,Gahl WA

Sun Jun 01 00:00:00 UTC 2008

Bioinformatics

Protein Aliases: Exophilin-3; leaden; Melanophilin; MGC2771; MGC59733; OTTMUSP00000025451; SlaC2-a; Slp homolog lacking C2 domains a; Synaptotagmin-like protein 2a

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Gene Aliases: MLPH; SLAC2-A; SLAC2A

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UniProt ID: (Human) Q9BV36

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Entrez Gene ID: (Human) 79083

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Function(s)

protein binding Rab GTPase binding metal ion binding

Process(es)

intracellular protein transport

Performance Guarantee

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Melanophilin Polyclonal Antibody

Melanophilin Antibody (10338-1-AP) in ICC/IF

Product Details

10338-1-AP

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (Paraffin) (IHC (P))

Immunocytochemistry (ICC/IF)

Immunoprecipitation (IP)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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