Invitrogen

Metadherin Polyclonal Antibody

Metadherin Antibody in Immunocytochemistry (ICC/IF)

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Metadherin Antibody (40-6500) in ICC/IF

Immunofluorescence analysis of Metadherin was done on 70% confluent log phase A549 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with Metadherin Rabbit Polyclonal Antibody (Product # 40-6500) at 2 µg/mL in 0.1% BSA and incubated for 3 hours at room tempe... View More

Metadherin Antibody in Immunohistochemistry (Paraffin) (IHC (P))

Metadherin Antibody in Western Blot (WB)

Metadherin Antibody in Western Blot, Immunocytochemistry (WB, ICC/IF)

Metadherin Antibody in Immunohistochemistry (IHC)

Metadherin Antibody in Western Blot, Immunoprecipitation (WB, IP)

Metadherin Antibody in Immunoprecipitation (IP)

Metadherin Antibody in ChIP Assay (ChIP)

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Product Details

40-6500

Applications

Tested Dilution

Publications

Western Blot (WB)

1-3 µg/mL

View 12 publications 12 publications

Immunohistochemistry (IHC)

View 4 publications 4 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:10-1:100

Immunocytochemistry (ICC/IF)

1-2 µg/mL

View 2 publications 2 publications

Immunoprecipitation (IP)

Assay-dependent

View 4 publications 4 publications

ChIP assay (ChIP)

View 1 publication 1 publication

RNA Immunoprecipitation (RIP)

View 1 publication 1 publication

Miscellaneous PubMed (MISC)

View 1 publication 1 publication

Product Specifications

Species Reactivity

Dog, Human, Mouse, Non-human primate

Published species

Human, Mouse

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Synthetic peptide derived from an internal region of the human, chimpanzee, mouse, and bovine metadherin (Mtdh, metastasis adhesion protein, LYsine-RIch CEACAM1 co-isolated (LYRIC), LYRIC/3D3, astrocyte elevated gene-1 (AEG-1)) protein, which differs from rat by one amino acid

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.25 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.4

Contains

0.1% sodium azide

Storage conditions

-20°C

Shipping conditions

Ambient (domestic); Wet ice (international)

AB_2533475

Target Information

Metadherin (Metastasis adhesion protein), also known as MTDH, LYsine-RIch CEACAM1 co-isolated (LYRIC), is a novel protein that localizes with the tight junction proteins ZO-1 and occludin in polarized epithelial cells. At the tight junction, it acts not as a structural component, but is rather recruited during the maturation of the tight junction complex. Metadherin is overexpressed in breast cancer tissue and breast tumor xenografts, while much lower levels are expressed in normal breast tissue. Metadherin binds to lung vasculature, one of the four common sites of breast cancer metastasis, through a C-terminal segment in the extracellular domain; blocking this lung-homing domain with antibodies or inhibiting metadherin with siRNA has been reported to inhibit breast cancer metastasis.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Small-molecule inhibitors that disrupt the MTDH-SND1 complex suppress breast cancer progression and metastasis.

Nature cancer

Shen M,Wei Y,Kim H,Wan L,Jiang YZ,Hang X,Raba M,Remiszewski S,Rowicki M,Wu CG,Wu S,Zhang L,Lu X,Yuan M,Smith HA,Zheng A,Bertino J,Jin JF,Xing Y,Shao ZM,Kang Y

40-6500 was used in Immunoprecipitation to demonstrate a significant therapeutic potential in targeting the MTDH-SND1 complex and identify a new class of therapeutic agents for metastatic breast cancer.

Sat Jan 01 00:00:00 UTC 2022

Pharmacological disruption of the MTDH-SND1 complex enhances tumor antigen presentation and synergizes with anti-PD-1 therapy in metastatic breast cancer.

Nature cancer

Shen M,Smith HA,Wei Y,Jiang YZ,Zhao S,Wang N,Rowicki M,Tang Y,Hang X,Wu S,Wan L,Shao ZM,Kang Y

40-6500 was used in RNA Immunoprecipitation, Immunocytochemistry, Immunohistochemistry (Paraffin), Immunoprecipitation, Western Blot to reveal a key role for this complex in suppressing antitumor T cell responses in breast cancer.

Sat Jan 01 00:00:00 UTC 2022

Disruption of SND1-MTDH Interaction by a High Affinity Peptide Results in SND1 Degradation and Cytotoxicity to Breast Cancer Cells In Vitro and In Vivo.

Molecular cancer therapeutics

Li P,He Y,Chen T,Choy KY,Chow TS,Wong ILK,Yang X,Sun W,Su X,Chan TH,Chow LMC

40-6500 was used in Immunoprecipitation, Western Blot to study the disruption of Staphylococcal nuclease domain-containing protein 1 (SND1)-metadherin (MTDH) interaction.

Fri Jan 01 00:00:00 UTC 2021

Disruption of SND1-MTDH Interaction by a High Affinity Peptide Results in SND1 Degradation and Cytotoxicity to Breast Cancer Cells In Vitro and In Vivo.

Molecular cancer therapeutics

Li P,He Y,Chen T,Choy KY,Chow TS,Wong ILK,Yang X,Sun W,Su X,Chan TH,Chow LMC

40-6500 was used in Immunoprecipitation, Western Blot to study the disruption of Staphylococcal nuclease domain-containing protein 1 (SND1)-metadherin (MTDH) interaction.

Fri Jan 01 00:00:00 UTC 2021

AEG-1 Regulates TWIK-1 Expression as an RNA-Binding Protein in Astrocytes.

Brain sciences

Jung HG,Kim A,Kim SC,Park JY,Hwang EM

40-6500 was used in Western Blot, Immunocytochemistry to investigate proteins directly regulated by AEG-1 by analyzing their RNA expression patterns in astrocytes transfected with scramble shRNA and AEG-1 shRNA.

Mon Jan 11 00:00:00 UTC 2021

AEG-1 Regulates TWIK-1 Expression as an RNA-Binding Protein in Astrocytes.

Brain sciences

Jung HG,Kim A,Kim SC,Park JY,Hwang EM

Mon Jan 11 00:00:00 UTC 2021

AEG-1 Regulates TWIK-1 Expression as an RNA-Binding Protein in Astrocytes.

Brain sciences

Jung HG,Kim A,Kim SC,Park JY,Hwang EM

Mon Jan 11 00:00:00 UTC 2021

AEG-1 Regulates TWIK-1 Expression as an RNA-Binding Protein in Astrocytes.

Brain sciences

Jung HG,Kim A,Kim SC,Park JY,Hwang EM

Mon Jan 11 00:00:00 UTC 2021

MTDH/AEG-1 downregulation using pristimerin-loaded nanoparticles inhibits Fanconi anemia proteins and increases sensitivity to platinum-based chemotherapy.

Gynecologic oncology

Bi J,Areecheewakul S,Li Y,Yang S,Zhang Y,Ebeid K,Li L,Thiel KW,Zhang J,Dai D,Salem AK,Leslie KK,Meng X

40-6500 was used in Western Blotting to discover that treatment with pristimerin in a novel nanoparticle formulation markedly inhibited tumor growth when combined with cisplatin.

Fri Nov 01 00:00:00 UTC 2019

Role of metadherin in estrogen-regulated gene expression.

International journal of molecular medicine

Li Y,Gonzalez Bosquet J,Yang S,Thiel KW,Zhang Y,Liu H,Leslie KK,Meng X

Published figure using Metadherin polyclonal antibody (Product # 40-6500) in Immunoprecipitation

Tue Aug 01 00:00:00 UTC 2017

Role of metadherin in estrogen-regulated gene expression.

International journal of molecular medicine

Li Y,Gonzalez Bosquet J,Yang S,Thiel KW,Zhang Y,Liu H,Leslie KK,Meng X

Published figure using Metadherin polyclonal antibody (Product # 40-6500) in Immunoprecipitation

Tue Aug 01 00:00:00 UTC 2017

Astrocyte Elevated Gene 1 Interacts with Acetyltransferase p300 and c-Jun To Promote Tumor Aggressiveness.

Molecular and cellular biology

Liu L,Guan H,Li Y,Ying Z,Wu J,Zhu X,Song L,Li J,Li M

406500 was used in ChIP assay, immunoprecipitation, and western blot to discuss how c-jun regulates astrocyte elevated gene-1 in gliomas

Wed Mar 01 00:00:00 UTC 2017

Astrocyte Elevated Gene 1 Interacts with Acetyltransferase p300 and c-Jun To Promote Tumor Aggressiveness.

Molecular and cellular biology

Liu L,Guan H,Li Y,Ying Z,Wu J,Zhu X,Song L,Li J,Li M

406500 was used in ChIP assay, immunoprecipitation, and western blot to discuss how c-jun regulates astrocyte elevated gene-1 in gliomas

Wed Mar 01 00:00:00 UTC 2017

Metadherin promotes metastasis by supporting putative cancer stem cell properties and epithelial plasticity in pancreatic cancer.

Oncotarget

Suzuki K,Takano S,Yoshitomi H,Nishino H,Kagawa S,Shimizu H,Furukawa K,Miyazaki M,Ohtsuka M

Published figure using Metadherin polyclonal antibody (Product # 40-6500) in Immunofluorescence

Tue Sep 12 00:00:00 UTC 2017

Metadherin promotes metastasis by supporting putative cancer stem cell properties and epithelial plasticity in pancreatic cancer.

Oncotarget

Suzuki K,Takano S,Yoshitomi H,Nishino H,Kagawa S,Shimizu H,Furukawa K,Miyazaki M,Ohtsuka M

Published figure using Metadherin polyclonal antibody (Product # 40-6500) in Immunofluorescence

Tue Sep 12 00:00:00 UTC 2017

Metadherin promotes metastasis by supporting putative cancer stem cell properties and epithelial plasticity in pancreatic cancer.

Oncotarget

Suzuki K,Takano S,Yoshitomi H,Nishino H,Kagawa S,Shimizu H,Furukawa K,Miyazaki M,Ohtsuka M

Published figure using Metadherin polyclonal antibody (Product # 40-6500) in Immunofluorescence

Tue Sep 12 00:00:00 UTC 2017

Overexpression of miR-506 suppresses proliferation and promotes apoptosis of osteosarcoma cells by targeting astrocyte elevated gene-1.

Oncology letters

Yao J,Qin L,Miao S,Wang X,Wu X

40-6500 was used in western blot determine the role of miR-506 in osteosarcoma pathogenesis

Thu Sep 01 00:00:00 UTC 2016

Metadherin contributes to the pathogenesis of chronic lymphocytic leukemia partially through Wnt/β-catenin pathway.

Medical oncology (Northwood, London, England)

Li PP,Feng LL,Chen N,Ge XL,Lv X,Lu K,Ding M,Yuan D,Wang X

40-6500 was used in western blot to investigate metadherin expression, significance, and related mechanism in chronic lymphocytic leukemia

Sun Feb 01 00:00:00 UTC 2015

Genetic Deficiency of Mtdh Gene in Mice Causes Male Infertility via Impaired Spermatogenesis and Alterations in the Expression of Small Non-coding RNAs.

The Journal of biological chemistry

Meng X,Yang S,Zhang Y,Wang X,Goodfellow RX,Jia Y,Thiel KW,Reyes HD,Yang B,Leslie KK

40-6500 was used in western blot to examine how metadherin contributes to drug resistance, metastasis, and angiogenesis.

Fri May 08 00:00:00 UTC 2015

Genetic Deficiency of Mtdh Gene in Mice Causes Male Infertility via Impaired Spermatogenesis and Alterations in the Expression of Small Non-coding RNAs.

The Journal of biological chemistry

Meng X,Yang S,Zhang Y,Wang X,Goodfellow RX,Jia Y,Thiel KW,Reyes HD,Yang B,Leslie KK

40-6500 was used in western blot to examine how metadherin contributes to drug resistance, metastasis, and angiogenesis.

Fri May 08 00:00:00 UTC 2015

Genetic Deficiency of Mtdh Gene in Mice Causes Male Infertility via Impaired Spermatogenesis and Alterations in the Expression of Small Non-coding RNAs.

The Journal of biological chemistry

Meng X,Yang S,Zhang Y,Wang X,Goodfellow RX,Jia Y,Thiel KW,Reyes HD,Yang B,Leslie KK

40-6500 was used in western blot to examine how metadherin contributes to drug resistance, metastasis, and angiogenesis.

Fri May 08 00:00:00 UTC 2015

MTDH-SND1 interaction is crucial for expansion and activity of tumor-initiating cells in diverse oncogene- and carcinogen-induced mammary tumors.

Cancer cell

Wan L,Lu X,Yuan S,Wei Y,Guo F,Shen M,Yuan M,Chakrabarti R,Hua Y,Smith HA,Blanco MA,Chekmareva M,Wu H,Bronson RT,Haffty BG,Xing Y,Kang Y

Published figure using Metadherin polyclonal antibody (Product # 40-6500) in Immunohistochemistry

Mon Jul 14 00:00:00 UTC 2014

MTDH-SND1 interaction is crucial for expansion and activity of tumor-initiating cells in diverse oncogene- and carcinogen-induced mammary tumors.

Cancer cell

Wan L,Lu X,Yuan S,Wei Y,Guo F,Shen M,Yuan M,Chakrabarti R,Hua Y,Smith HA,Blanco MA,Chekmareva M,Wu H,Bronson RT,Haffty BG,Xing Y,Kang Y

Published figure using Metadherin polyclonal antibody (Product # 40-6500) in Immunohistochemistry

Mon Jul 14 00:00:00 UTC 2014

MTDH-SND1 interaction is crucial for expansion and activity of tumor-initiating cells in diverse oncogene- and carcinogen-induced mammary tumors.

Cancer cell

Wan L,Lu X,Yuan S,Wei Y,Guo F,Shen M,Yuan M,Chakrabarti R,Hua Y,Smith HA,Blanco MA,Chekmareva M,Wu H,Bronson RT,Haffty BG,Xing Y,Kang Y

Published figure using Metadherin polyclonal antibody (Product # 40-6500) in Immunohistochemistry

Mon Jul 14 00:00:00 UTC 2014

Genetic ablation of metadherin inhibits autochthonous prostate cancer progression and metastasis.

Cancer research

Wan L,Hu G,Wei Y,Yuan M,Bronson RT,Yang Q,Siddiqui J,Pienta KJ,Kang Y

Published figure using Metadherin polyclonal antibody (Product # 40-6500) in Western Blot

Mon Sep 15 00:00:00 UTC 2014

Genetic ablation of metadherin inhibits autochthonous prostate cancer progression and metastasis.

Cancer research

Wan L,Hu G,Wei Y,Yuan M,Bronson RT,Yang Q,Siddiqui J,Pienta KJ,Kang Y

Published figure using Metadherin polyclonal antibody (Product # 40-6500) in Western Blot

Mon Sep 15 00:00:00 UTC 2014

Exposure to cadmium chloride influences astrocyte-elevated gene-1 (AEG-1) expression in MDA-MB231 human breast cancer cells.

Biochimie

Luparello C,Longo A,Vetrano M

Sun Jan 01 00:00:00 UTC 2012

Bioinformatics

Protein Aliases: 3D3/LYRIC; AEG-1; astrocyte elevated gene 1; Astrocyte elevated gene-1 protein; LYRIC/ MTDH; Lysine-rich CEACAM1 co-isolated protein; Metadherin; Metastasis adhesion protein; Protein LYRIC

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Gene Aliases: 2610103J23Rik; 3D3; 3D3/Lyric; AEG-1; AEG1; AV353288; D8Bwg1112e; LYRIC; LYRIC/3D3; MTDH

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UniProt ID: (Human) Q86UE4, (Mouse) Q80WJ7

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Entrez Gene ID: (Dog) 612147, (Human) 92140, (Mouse) 67154

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Function(s)

RNA polymerase II transcription factor binding transcription coactivator activity double-stranded RNA binding protein binding poly(A) RNA binding NF-kappaB binding

Process(es)

negative regulation of transcription from RNA polymerase II promoter positive regulation of autophagy lipopolysaccharide-mediated signaling pathway negative regulation of apoptotic process positive regulation of I-kappaB kinase/NF-kappaB signaling positive regulation of angiogenesis positive regulation of NF-kappaB transcription factor activity positive regulation of protein kinase B signaling bicellular tight junction assembly

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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Metadherin Polyclonal Antibody

Metadherin Antibody (40-6500) in ICC/IF

Product Details

40-6500

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (Paraffin) (IHC (P))

Immunocytochemistry (ICC/IF)

Immunoprecipitation (IP)

ChIP assay (ChIP)

RNA Immunoprecipitation (RIP)

Miscellaneous PubMed (MISC)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Target Information

Bioinformatics

Performance Guarantee

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