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Invitrogen
Metallothionein Monoclonal Antibody (8D8)
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Metallothionein Antibody (MA5-27587) in WB
Western blot analysis of Metallothionein in pseudomonas aeruginosa metallothionein (pmta) gst tagged using 1 µg of protein. Sample was prepared with 5% milk and TBST, incubated with Metallothionein monoclonal antibody (Product # MA5-27587) using a dilution of 1:1000 (2 hours at RT) and followed by a Goat Anti-Mouse HRP secondary antibody at a dilution of 1:5000 (1 hour at RT). Samples are arranged as follows: Lane 1: molecular weight ladder, Lane 2: GST, Lane 3: Purified Pseudomonas aeruginosa Metallothionein (PmtA) GST tagged.
Product Details
MA5-27587
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Bacteria
Host/Isotype
Mouse
/ IgG1
Class
Monoclonal
Type
Antibody
Clone
8D8
Immunogen
Full length Pseudomonas aeruginosa Metallothionein protein
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Protein G
Storage buffer
PBS, pH 7.4, with 50% glycerol
Contains
0.09% sodium azide
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2735269
Product Specific Information
A 1:1,000 dilution of MA5-27587 was sufficient for detection of Metallothionein in 1 µg of purified Pseudomonas aeruginosa Metallothionein (PmtA)-GST tagged protein by ECL immunoblot analysis using goat anti-mouse IgG:HRP as the secondary antibody.|Detects 36 kDa band corresponding to Pseudomonas aeruginosa Metallothionein.
Target Information
This gene is a member of the metallothionein family of genes. Proteins encoded by this gene family are low in molecular weight, are cysteine-rich, lack aromatic residues, and bind divalent heavy metal ions. The conserved cysteine residues co-ordinate metal ions using mercaptide linkages. These proteins act as anti-oxidants, protect against hydroxyl free radicals, are important in homeostatic control of metal in the cell, and play a role in detoxification of heavy metals. Disruption of two metallothionein genes in mouse resulted in defects in protection against heavy metals, oxidative stress, immune reactions, carcinogens, and displayed obesity. MT1A (Metallothionein 1A) is a Protein Coding gene. Diseases associated with MT1A include Menkes Disease and Deficiency Anemia. Among its related pathways are Metal ion SLC transporters and Cellular responses to stimuli. An important paralog of this gene is MT2A.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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