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MiTF Antibody (PA5-81221) in ICC/IF
Immunofluorescence staining of MiTF in HeLa cells. Cells were fixed with 4% PFA, blocked with 10% serum, and then incubated with MiTF Polyclonal Antibody (Product # PA5-81221, 1:1,000)(Figure A) or MiTF Polyclonal Antibody and antigen (Figure B) at 4°C overnight. Then cells were stained with the Alexa Fluor®488-conjugated Goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI (blue). Positive staining was localized to nucleus.
Product Details
PA5-81221
Product Specifications
Species Reactivity
Human
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
A synthetic peptide corresponding to the center region of the Human MITF
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Protein A, Antigen affinity chromatography
Storage buffer
PBS
Contains
no preservative
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2788450
Product Specific Information
This product is preservative free. It is recommended to add sodium azide to avoid contamination (final concentration 0.05%-0.1%).
This antibody has specificity for Human MITF.
Target Information
Mi is a basic helix-loop-helix-leucine zipper (b-HLH-ZIP) transcription factor implicated in pigmentation, mast cells and bone development. The mutation of Mi causes Waardenburg Syndrome type II in humans. In mice, a profound loss of pigmented cells in the skin eye and inner ear results, as well as osteopetrosis and defects in natural killer and mast cells. There are two known isoforms of Mi differing by 66 amino acids at the NH2 terminus. Shorter forms are expressed in melanocytes and run as two bands at 52 kDa and 56 kDa, while the longer Mi form runs as a cluster of bands at 60-70 kDa in osteoclasts and in B16 melonoma cells (but not other melanoma cell lines), as well as mast cells and heart.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: bHLHe32; Class E basic helix-loop-helix protein 32; microphtalmia-associated transcription factor; microphthalmia; Microphthalmia-associated transcription factor
Gene Aliases: BHLHE32; CMM8; MI; MITF; WS2; WS2A
UniProt ID: (Human) O75030
Entrez Gene ID: (Human) 4286
RNA polymerase II core promoter proximal region sequence-specific DNA binding
transcriptional activator activity, RNA polymerase II core promoter proximal region sequence-specific binding
transcriptional repressor activity, RNA polymerase II transcription regulatory region sequence-specific binding
protein binding
protein dimerization activity
negative regulation of transcription from RNA polymerase II promoter
regulation of transcription, DNA-templated
transcription from RNA polymerase II promoter
protein complex assembly
multicellular organism development
positive regulation of gene expression
protein sumoylation
melanocyte differentiation
regulation of apoptotic process
regulation of osteoclast differentiation
positive regulation of transcription, DNA-templated
positive regulation of transcription from RNA polymerase II promoter
mast cell migration
positive regulation of DNA-templated transcription, initiation
regulation of RNA biosynthetic process
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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