Invitrogen

Mitochondria Membrane Integrity Antibody Cocktail

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Datasheet
Protocols
Questions & Answers
Datasheet
Protocols
Questions & Answers

Product Details

45-7799

Applications
Tested Dilution
Publications

Western Blot (WB)

10.2 µg/mL
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Product Specifications

Species Reactivity

Bovine, Human, Mouse, Rat

Host/Isotype

Mouse / Ig

Class

Cocktail

Type

Antibody

Clone

Cocktail

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

2.37 mg/mL

Purification

IgG fraction

Storage buffer

HEPES buffered saline

Contains

0.02% sodium azide

Storage conditions

4°C

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2533833

Product Specific Information

Mitochondria Membrane Integrity cocktail of monoclonal antibodies specific to human, bovine, mouse, rat mitochondria membranes. This antibody is validated for use in Western Blot, and can be used to assess mitochondrial membrane integrity during and after purification.

Contains:

50 µg of Porin clone #2B12AF12 - Human VDAC1. Concentration 2 µg/mL.

50 µg of Cyclophilin D clone #E11AE12BD4 - Rat CypD/PPIF. Concentration 2 µg/mL.

50 µg of C-III-Core 1 clone # 16D10AD9AH5 - Bovine Heart Complex II/III. Concentration 2 µg/mL.

5 µg of Cytochrome C clone #37BA11 - Human Cytochrome c. Concentration 0.1 µg/mL.

100 µg of C-V-a clone #7H10BD4F9 - Bovine Complex V. Concentration 4 µg/mL.

This antibody cocktail (2.37 mg/mL) should be diluted to a final working concentration of 10.2 µg/mL for use in Western blot applications.

Target Information

Voltage-dependent anion channel (VDAC) proteins are abundant, pore-forming proteins belonging to the eukaryotic mitochondrial porins. It was discovered in the mitochondrial outer membrane. It also is expressed in the plasma membrane. At least three different VDAC genes have been identified in vertebrates. VDAC proteins are known to play an essential role in cellular metabolism and in the early stages of apoptosis. For example, VDAC contitutes a major pathway by which metabolites such as ADP/ATP, succinate and citrate are exchanged between the cytosol and mitochondria. And VDAC1 in the plasma membrane establishes a novel level of apoptosis regulation putatively via its redox activity.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Bioinformatics

Protein Aliases: 16D10AD9AH5; 20B12AF2; 37BA11; 7H10BD4F9; BR1-VDAC; Brain-derived voltage-dependent anion channel 1; Complex III subunit 4; Complex III subunit IV; cyclophilin 3; Cyclophilin D; Cyclophilin F; CyP-D; CyP-M; Cytochrome b-c1 complex subunit 4; Cytochrome c; Cytochrome C, expressed in somatic tissues; Cytochrome c, somatic; Cytochrome c-1; Cytochrome c1, heme protein, mitochondrial; E11AE12BD4; Mitochondrial cyclophilin; mitochondrial Cyclophilin D; mVDAC1; mVDAC5; Non-selective voltage-gated ion channel VDAC1; Outer mitochondrial membrane protein porin 1; Peptidyl-prolyl cis-trans isomerase F, mitochondrial; peptidyl-prolyl cis-trans isomerase, mitochondrial; peptidylprolyl isomerase F (cyclophilin F); Plasmalemmal porin; Porin 31HL; Porin 31HM; PPIase F; Rotamase F; rVDAC1; Ubiquinol-cytochrome-c reductase complex cytochrome c1 subunit; VDAC-1; VDAC-5; Voltage-dependent anion-selective channel protein 1; Voltage-dependent anion-selective channel protein 5

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Gene Aliases: 2610002H19Rik; AA408921; AL033343; AW457192; BOS_13658; BOS_24179; BOS_4320; BOS_8120; CYC; CYC1; CYCS; CYCSA; CyP-D; CyP-F; CyP-M; CYP3; CypD; cytc; HCS; MC3DN6; PORIN; PPIase; PPIF; THC4; UQCR4; VDAC; VDAC-1; VDAC1; Vdac5

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UniProt ID: (Human) P08574, (Bovine) P00125, (Human) P99999, (Bovine) P62894, (Mouse) Q9D0M3, (Mouse) P62897, (Rat) P62898, (Human) P30405, (Bovine) P30404, (Rat) P29117, (Mouse) Q99KR7, (Human) P21796, (Bovine) P45879, (Mouse) Q60932, (Rat) Q9Z2L0

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Entrez Gene ID: (Human) 1537, (Bovine) 512500, (Human) 54205, (Bovine) 510767, (Rat) 300047, (Mouse) 66445, (Mouse) 13063, (Rat) 25309, (Human) 10105, (Bovine) 414346, (Rat) 282819, (Mouse) 105675, (Human) 7416, (Bovine) 282119, (Mouse) 22333, (Rat) 83529

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Function(s)
heme binding electron transporter, transferring electrons from CoQH2-cytochrome c reductase complex and cytochrome c oxidase complex activity metal ion binding iron ion binding electron carrier activity protein serine/threonine phosphatase activity protein binding molecular_function lipid binding peptidyl-prolyl cis-trans isomerase activity cyclosporin A binding peptide binding isomerase activity voltage-gated anion channel activity porin activity protein kinase binding protein complex binding ion channel binding nucleotide binding anion channel activity
Process(es)
response to glucagon behavioral fear response transport ion transport anion transport mitochondrial calcium ion transport apoptotic process chemical synaptic transmission neuron-neuron synaptic transmission learning regulation of anion transport transmembrane transport anion transmembrane transport regulation of anion transmembrane transport epithelial cell differentiation ion transmembrane transport regulation of ion transmembrane transport response to reactive oxygen species mitochondrial electron transport, ubiquinol to cytochrome c mitochondrial electron transport, cytochrome c to oxygen protein dephosphorylation mitochondrion organization activation of cysteine-type endopeptidase activity involved in apoptotic process by cytochrome c cellular respiration intrinsic apoptotic signaling pathway protein peptidyl-prolyl isomerization response to ischemia protein folding apoptotic mitochondrial changes regulation of proton-transporting ATPase activity, rotational mechanism regulation of necrotic cell death negative regulation of ATPase activity negative regulation of apoptotic process regulation of mitochondrial membrane permeability necroptotic process cellular response to hydrogen peroxide cellular response to arsenic-containing substance cellular response to calcium ion positive regulation of release of cytochrome c from mitochondria negative regulation of release of cytochrome c from mitochondria negative regulation of oxidative phosphorylation regulation of mitochondrial membrane permeability involved in programmed necrotic cell death negative regulation of oxidative phosphorylation uncoupler activity negative regulation of intrinsic apoptotic signaling pathway apoptotic DNA fragmentation hydrogen peroxide metabolic process ATP synthesis coupled electron transport viral process macroautophagy regulation of mitophagy negative regulation of reactive oxygen species metabolic process response to oxidative stress regulation of apoptotic process programmed cell death oxidation-reduction process electron transport chain positive regulation of necrotic cell death negative regulation of hydrogen peroxide biosynthetic process glial cell apoptotic process positive regulation of cellular respiration
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