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Invitrogen
Moesin Monoclonal Antibody (8D4)
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FIGURE: 1 / 8
Moesin Antibody (MA5-49167) in ICC/IF
Immunocytochemistry analysis of Moesin in SiHa cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent for 15 mins. The cells were blocked with 10% goat serum. Samples were then incubated in Moesin Monoclonal antibody (Product # MA5-49167) using a dilution of 5 μg/mL. DyLight®488 Conjugated Goat Anti-Mouse IgG was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate f... View More
Product Details
MA5-49167
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Non-human primate,
Rat
Host/Isotype
Mouse
/ IgG1
Class
Monoclonal
Type
Antibody
Clone
8D4
Immunogen
E.coli-derived human Moesin/MSN recombinant protein (Position: R184-K568).
Conjugate
Form
Lyophilized
Concentration
500 µg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS with 4mg trehalose
Contains
no preservative
Storage conditions
-20°C
Shipping conditions
Wet ice
RRID
AB_3074825
Product Specific Information
Adding 0.2 mL of distilled water will yield a concentration of 500 µg/mL.
Positive Control - WB: human HL-60 whole cell, human K562 whole cell, monkey COS-7 whole cell, rat kidney tissue, mouse kidney tissue, human MCF-7 whole cell. IHC: human gastric adenocarcinoma tissue, human colonic adenocarcinoma tissue, human liver cancer tissue, human placenta tissue. ICC/IF: SiHa cell. Flow: U87 cell.|Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.
Target Information
Moesin, a member of the talin-4. 1 superfamily, is a linking protein of the submembraneous actin cytoskeleton. It is expressed in macrophages, lymphocytes, fibroblastic, endothelial, epithelial, and neuronal cell lines but not in blood cells. It is involved in cell adhesion, migration, and organization of cell surface structures.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: epididymis luminal protein 70; Membrane-organizing extension spike protein; Moesin
Gene Aliases: C78546; HEL70; MSN
UniProt ID: (Human) P26038, (Rat) O35763, (Mouse) P26041
Entrez Gene ID: (Human) 4478, (Rat) 81521, (Mouse) 17698
leukocyte cell-cell adhesion
positive regulation of gene expression
membrane to membrane docking
leukocyte migration
establishment of endothelial barrier
regulation of lymphocyte migration
regulation of organelle assembly
positive regulation of protein localization to early endosome
positive regulation of cellular protein catabolic process
positive regulation of early endosome to late endosome transport
movement of cell or subcellular component
cytoskeleton organization
regulation of cell shape
regulation of cell size
gland morphogenesis
establishment of epithelial cell apical/basal polarity
cellular response to testosterone stimulus
positive regulation of podosome assembly
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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