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          • Primary Antibodies ›
          • Histone 1F0 Antibodies

          Invitrogen

          Mono-Methyl-Histone 1F0 (Lys101) Polyclonal Antibody

          View all (15) Histone 1F0 antibodies
          Datasheet
          Protocols
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          Datasheet
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          Cite Mono-Methyl-Histone 1F0 (Lys101) Polyclonal Antibody

          • Antibody Testing Data (4)
          Mono-Methyl-Histone 1F0 (Lys101) Antibody in Immunocytochemistry (ICC/IF)
          Group 53 Created with Sketch.
          Mono-Methyl-Histone 1F0 (Lys101) Antibody in Immunocytochemistry (ICC/IF)
          Group 53 Created with Sketch.

          FIGURE: 1 / 4

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          Mono-Methyl-Histone 1F0 (Lys101) Antibody (PA5-112436) in ICC/IF

          Immunocytochemichal analysis of Methyl Histone 1F0 (Lys101) in Hela cells using a Polyclonal antibody (Product # PA5-112436) at a dilution of 1:5. Hela cells performed on a Leica BondTM system. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detecte... View More {{ $ctrl.currentElement.advancedVerification.fullName }} validation info. View more
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          Mono-Methyl-Histone 1F0 (Lys101) Antibody in Immunocytochemistry (ICC/IF)
          Mono-Methyl-Histone 1F0 (Lys101) Antibody in Immunocytochemistry (ICC/IF)
          Mono-Methyl-Histone 1F0 (Lys101) Antibody in Western Blot (WB)
          Mono-Methyl-Histone 1F0 (Lys101) Antibody in ChIP Assay (ChIP)

          Product Details

          PA5-112436

          Applications
          Tested Dilution
          Publications

          Western Blot (WB)

          1:50-1:500
          -

          Immunocytochemistry (ICC/IF)

          1:10
          -

          ELISA (ELISA)

          Assay-dependent
          -

          ChIP assay (ChIP)

          Assay-dependent
          -
          Product Specifications

          Species Reactivity

          Human

          Host/Isotype

          Rabbit / IgG

          Class

          Polyclonal

          Type

          Antibody

          Immunogen

          Peptide sequence around site of Mono-methyl-Lys (101) derived from Human Histone H1.0
          View immunogen

          Conjugate

          Unconjugated Unconjugated Unconjugated

          Form

          Liquid

          Concentration

          0.1 mg/mL

          Purification

          Antigen affinity chromatography

          Storage buffer

          PBS, pH 7.4, with 50% glycerol

          Contains

          0.03% ProClin 300

          Storage conditions

          -20°C or -80°C if preferred

          Shipping conditions

          Wet ice

          RRID

          AB_2867171

          Target Information

          Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a replication-independent histone that is a member of the histone H1 family.

          For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

          References (0)

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          Cite this product

          Bioinformatics

          Protein Aliases: H1 histone family, member 0; H1.0, H1(0), H1-0; Histone H1'; Histone H1(0); Histone H1.0

          View more View less

          Gene Aliases: H1-0; H10; H1F0; H1FV

          View more View less

          UniProt ID: (Human) P07305

          View more View less

          Entrez Gene ID: (Human) 3005

          View more View less

          Function(s)
          protein binding chromatin DNA binding poly(A) RNA binding
          Process(es)
          apoptotic DNA fragmentation nucleosome assembly
          It has to be done as per old AB suggested Products section.
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          Performance Guarantee

          If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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