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          • Isotype Controls ›
          • Mouse IgG1 kappa Isotype Controls

          Invitrogen

          Mouse IgG1 kappa Isotype Control (P3.6.2.8.1), FITC, eBioscience™

          5 Published Figures
          View all Mouse IgG1 kappa Isotype Controls

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          Cite Mouse IgG1 kappa Isotype Control (P3.6.2.8.1), FITC, eBioscience™

          • Testing Data (2)
          • Published Figures (5)
          Mouse IgG1 kappa Isotype Control in Immunocytochemistry (ICC/IF)
          Group 53 Created with Sketch.
          Mouse IgG1 kappa Isotype Control in Immunocytochemistry (ICC/IF)
          Group 53 Created with Sketch.

          FIGURE: 1 / 7

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          Mouse IgG1 kappa Isotype Control (11-4714-42) in ICC/IF

          Immunocytochemistry of fixed and permeabilized C6 cells using 1 µg/mL of Mouse IgG1 Isotype Control FITC (Product # 11-4714-42) (left) or 1 µg/mL of Anti-Vimentin FITC (right). Nuclei are counterstained with DAPI. {{ $ctrl.currentElement.advancedVerification.fullName }} validation info. View more
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          Mouse IgG1 kappa Isotype Control in Immunocytochemistry (ICC/IF)
          Mouse IgG1 kappa Isotype Control in Immunocytochemistry (ICC/IF)
          Mouse IgG1 kappa Isotype Control in Flow Cytometry (Flow)
          Mouse IgG1 kappa Isotype Control in Flow Cytometry (Flow)
          Mouse IgG1 kappa Isotype Control in Flow Cytometry (Flow)
          Mouse IgG1 kappa Isotype Control in Flow Cytometry (Flow)
          Mouse IgG1 kappa Isotype Control in Flow Cytometry (Flow)

          Product Details

          11-4714-42

          Applications
          Tested Dilution

          Immunohistochemistry (IHC)

          Assay-Dependent

          Immunocytochemistry (ICC/IF)

          Assay-Dependent

          Flow Cytometry (Flow)

          5 µL (1 µg)/test

          Control (Ctrl)

          Assay-Dependent
          Product Specifications

          Host/Isotype

          Mouse / IgG1, kappa

          Class

          Monoclonal

          Type

          Isotype Control

          Clone

          P3.6.2.8.1

          Conjugate

          FITC FITC FITC
          • Unconjugated
          • Alexa Fluor 488 
          • Alexa Fluor 532 
          • Alexa Fluor 561
          • Alexa Fluor 647 
          • Alexa Fluor 660 
          • Alexa Fluor 700 
          • APC 
          • APC-eFluor 780
          • Biotin 
          • Brilliant UV 395
          • Brilliant UV 496
          • Brilliant UV 563
          • Brilliant UV 615
          • Brilliant UV 661
          • Brilliant UV 737
          • Brilliant UV 805
          • Brilliant Violet 421
          • Brilliant Violet 480
          • Brilliant Violet 605
          • Brilliant Violet 650
          • Brilliant Violet 711
          • Brilliant Violet 786
          • eFluor 450
          • eFluor 506
          • eFluor 570
          • eFluor 615
          • eFluor 660
          • Functional Grade
          • Pacific Orange
          • PE 
          • PE-Cyanine5
          • PE-Cyanine5.5
          • PE-Cyanine7
          • PE-eFluor 610
          • PerCP-Cyanine5.5
          • PerCP-eFluor 710
          • Super Bright 436
          • Super Bright 600
          • Super Bright 645
          • Super Bright 702
          • Super Bright 780
          • Request custom conjugation

          Excitation/Emission Max

          498/517 nm View spectra spectra

          Form

          Liquid

          Concentration

          5 µL/Test

          Purification

          Affinity chromatography

          Storage buffer

          PBS, pH 7.2, with 0.2% BSA

          Contains

          0.09% sodium azide

          Storage conditions

          4°C, store in dark, DO NOT FREEZE!

          Shipping conditions

          Ambient (domestic); Wet ice (international)

          RRID

          AB_10596964

          Product Specific Information

          Description: The monoclonal mouse IgG1 K immunoglobulin is useful as an isotype control.

          Applications Reported:FITC Mouse IgG1 K Isotype Control has been reported for use in immunocytochemistry, immunohistochemistry, and flow cytometric analysis.

          Applications Tested: Has been pre-titrated and can be used at 5 µL (1 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.

          Excitation: 488 nm; Emission: 520 nm; Laser: Blue Laser.

          Filtration: 0.2 µm post-manufacturing filtered.

          Target Information

          Mouse IgG1 is a subtype of immunoglobulin G antibody that is produced by B cells in response to an antigen. It is the most abundant IgG subtype in the serum and plays a crucial role in the adaptive immune response. Mouse IgG1 is composed of two heavy chains and two light chains, and it is involved in opsonization, complement activation, and antibody-dependent cell-mediated cytotoxicity. It is also involved in the regulation of immune responses, including the activation of T cells and the inhibition of B cell activation. Mouse IgG1 is widely used in research and diagnostic applications, including ELISA, Western blotting, and immunohistochemistry. It is also used in therapeutic applications, such as immunotherapy and vaccine development.

          For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

          View product citations for antibody 11-4714-42 on CiteAb

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          Cite this product

          Bioinformatics

          Protein Aliases: Immunoglobulin G; Immunoglobulin G1; ImmunoglobulinG; ImmunoglobulinG1

          View more View less

          It has to be done as per old AB suggested Products section.
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