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Invitrogen
Mouse anti-Human IgG1 Secondary Antibody
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FIGURE: 1 / 5
Human IgG1 Secondary Antibody (MA1-34581) in ICC/IF
Immunofluorescence analysis of Mouse anti-Human IgG1 Secondary Antibody (Product # MA1-34581) was performed using T-47D cells stained with Human MUC1 antibody (Product # MA5- 42157). The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, blocked with 1% BSA for 1 hour and labeled with primary antibody (1:100 dilution) for 3 hours at room temperatu... View More
Product Details
MA1-34581
Applications
Tested Dilution
Western Blot (WB)
1:1000
Immunohistochemistry (Frozen) (IHC (F))
Assay-dependent
Immunocytochemistry (ICC/IF)
1:2000
ELISA (ELISA)
Assay-dependent
Product Specifications
Species Reactivity
Human
Host/Isotype
Mouse
/ IgG1
Class
Monoclonal
Type
Secondary Antibody
Clone
2C11
Immunogen
IgG from human serum.
Conjugate
Form
Liquid
Concentration
5.4 mg/mL
Purification
Protein A
Storage buffer
PBS, pH 7.4
Contains
0.09% sodium azide
Storage conditions
4°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_11004658
Target
IgG1
Antibody Form
Whole Antibody
Product Specific Information
MA1-34581 detects IgG1 from human samples.
Target Information
Anti-Human secondary antibodies are affinity-purified antibodies with well-characterized specificity for human immunoglobulins and are useful in the detection, sorting or purification of its specified target. Secondary antibodies offer increased versatility enabling users to use many detection systems (e.g. HRP, AP, fluorescence). They can also provide greater sensitivity through signal amplification as multiple secondary antibodies can bind to a single primary antibody. Most commonly, secondary antibodies are generated by immunizing the host animal with a pooled population of immunoglobulins from the target species and can be further purified and modified (i.e. immunoaffinity chromatography, antibody fragmentation, label conjugation, etc.) to generate highly specific reagents.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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