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Mre11 Antibody (600-401-872) in ChIP
Chromatin Immunoprecipitation (ChIP) using Rocklands Affinity Purified Mre11 (S. cerevisiae) antibody. A yeast strain containing the HO endonuclease gene controlled by a galactose-inducible promoter (uninduced 0 m lanes) was shifted into galactose containing medium (induced 60 m lanes). After 1 hour of induction cells were cross-linked with formaldehyde followed by preparation of sheared chromatin. Chromatin was immunoprecipitated with the antibody at the stated dilutions. Immunocomplexes were captured using polyacrylamide bead linked sec... View More
Product Details
600-401-872
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Yeast
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to an internal region near amino acids 575-600 of Saccharomyces cerevisiae (baker's yeast) Mre11 protein.
Conjugate
Form
Liquid
Concentration
1.17 mg/mL
Purification
Affinity chromatography
Storage buffer
0.02M potassium phosphate, pH 7.2, with 0.15M NaCl
Contains
0.01% sodium azide
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Wet ice
Product Specific Information
Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
This affinity-purified antibody is directed against Saccharomyces cerevisiae Mre11 protein. A BLAST analysis was used to suggest that little cross reactivity should be expected with this protein from other sources.
Target Information
MRE11 (double-strand break repair protein MRE11) is a component of the MRN complex. This complex plays a central role in double-strand break repair, DNA recombination, maintenance of telomere integrity and meiosis. The complex possesses single-stranded endonuclease activity and double-strand-specific 3'-5' exonuclease activity which are provided by MRE11. RAD50 is required to bind DNA ends and hold them in close proximity. Mutations in the gene could result in ataxia-telangiectasia-like disorder 1.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Double-strand break repair protein MRE11; Mre11p
Gene Aliases: MRE11; NGS1; RAD58; XRS4; YM9959.06C; YMR224C
UniProt ID: (Baker's yeast) P32829
Entrez Gene ID: (Baker's yeast) 855264
double-stranded telomeric DNA binding
adenylate kinase activity
nuclease activity
endonuclease activity
endodeoxyribonuclease activity
exonuclease activity
3'-5' exonuclease activity
hydrolase activity
manganese ion binding
protein complex scaffold
telomeric DNA binding
single-stranded telomeric DNA binding
G-quadruplex DNA binding
meiotic DNA double-strand break processing
double-strand break repair via break-induced replication
DNA metabolic process
DNA repair
base-excision repair
double-strand break repair
double-strand break repair via nonhomologous end joining
cellular response to DNA damage stimulus
meiotic nuclear division
reciprocal meiotic recombination
ascospore formation
meiotic DNA double-strand break formation
regulation of transcription involved in meiotic cell cycle
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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