Search Thermo Fisher Scientific
Disclaimer
Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.
Invitrogen
Myc Tag Monoclonal Antibody (Myc.A7), DyLight™ 800 4X PEG
FIGURE: 1 / 4
Myc Tag Antibody (MA1-21316-D800) in WB
Western blot analysis of Myc Epitope Tag was performed by loading 2 µg of E. coli lysate containing a Myc-epitope tagged protein onto a 4-20% Tris-HCl polyacrylamide gel. Proteins were transferred to a low fluorescence PVDF membrane (Product # 22860) and blocked with SEA BLOCK (Product # 37527) for at least 1 hour. The membrane was probed with a DyLight 800-conjugated Myc Epitope Tag monoclonal antibody (Product # MA1-21316-D800) at a dilution of 1:500 overnight at 4°C on a rocking platform and washed in TBS-0.1% Tween-20. Fluorescent dete... View More
Product Details
MA1-21316-D800
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Tag
Published species
Tag
Host/Isotype
Mouse
/ IgG1
Class
Monoclonal
Type
Antibody
Clone
Myc.A7
Immunogen
EQKLISEEDL synthetic peptide conjugated to KLH
Conjugate
Excitation/Emission Max
789/794 nm
View spectra
Form
Liquid
Concentration
1 mg/mL
Purification
Protein A
Storage buffer
MES with proprietary stabilizer
Contains
no preservative
Storage conditions
4°C, do not freeze
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2536996
Product Specific Information
MA1-21316-D800 detects Myc tag from various samples. MA1-21316-D800 has been successfully used for fluorescent detection of Myc tagged proteins in Western Blotting.
DyLight 800 4X PEG has an excitation/emission of 783/797 nm.
Target Information
Myc is a proto-oncogene and encodes a nuclear phosphoprotein that plays a role in cell cycle progression, apoptosis and cellular transformation. The encoded protein forms a heterodimer with the related transcription factor MAX. This complex binds to the E box DNA consensus sequence and regulates the transcription of specific target genes. Amplification of this gene is frequently observed in numerous human cancers. Translocations involving this gene are associated with Burkitt lymphoma and multiple myeloma in human patients. There is evidence to show that translation initiates both from an upstream, in-frame non-AUG (CUG) and a downstream AUG start site, resulting in the production of two isoforms with distinct N-termini.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: 46-0603; c Myc; c-myc epitope tag; cmyc; Myc protein; Myc Tag; R950-25; R95025
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
Learn more
We're here to help
Get expert recommendations for common problems or connect directly with an on staff expert for technical assistance related to applications, equipment and general product use.
Contact tech support