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Invitrogen
Myc Tag Monoclonal Antibody (Myc.A7), HRP
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Myc Tag Antibody (MA1-21316-HRP) in WB
Western blot analysis of Myc was performed by loading 50 µg of NCCIT and Jurkat lysates and 10 µg of Myc-MYCBP-containing cell lysate onto an SDS polyacrylamide gel. Proteins were transferred to a PVDF membrane. The membrane was blocked, and probed with an HRP-conjugated Myc Epitope Tag antibody (Product # MA1-21316-HRP) at a dilution of 1:1000 overnight at 4C. Chemiluminescent detection was performed using SuperSignal West Dura (Product # 34075) at an exposure time of 2 minutes (NCCIT and Jurkat) and 3 seconds (Myc-MYCBP). Results show a ... View More
Product Details
MA1-21316-HRP
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Tag
Published species
Tag
Host/Isotype
Mouse
/ IgG1
Class
Monoclonal
Type
Antibody
Clone
Myc.A7
Immunogen
Synthetic peptide: EQKLISEEDL (Myc)
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Protein A
Storage buffer
PBS
Contains
0.07% Kathon
Storage conditions
4°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2536997
Product Specific Information
MA1-21316-HRP has been successfully used in Western blotting applications on myc tagged samples.
Target Information
Myc is a proto-oncogene and encodes a nuclear phosphoprotein that plays a role in cell cycle progression, apoptosis and cellular transformation. The encoded protein forms a heterodimer with the related transcription factor MAX. This complex binds to the E box DNA consensus sequence and regulates the transcription of specific target genes. Amplification of this gene is frequently observed in numerous human cancers. Translocations involving this gene are associated with Burkitt lymphoma and multiple myeloma in human patients. There is evidence to show that translation initiates both from an upstream, in-frame non-AUG (CUG) and a downstream AUG start site, resulting in the production of two isoforms with distinct N-termini.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: 46-0603; c Myc; c-myc epitope tag; cmyc; Myc protein; Myc Tag; R950-25; R95025
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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