Invitrogen

Myogenin Monoclonal Antibody (F5D)

Myogenin Antibody in Immunocytochemistry (ICC/IF)

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Myogenin Antibody (MA5-11486) in ICC/IF

Immunofluorescence analysis of Myogenin was performed using 70% confluent log phase RD cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 1 hour at room temperature. The cells were labeled with Myogenin Mouse Monoclonal Antibody (F5D) Product # MA5-11486) at 5 µg/mL in 0.1% BSA, incubated at 4 degree Celsius overnight and then with Goat anti-Mouse IgG (H+L) Superclonal™ Recombinant Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A2... View More

Myogenin Antibody in Immunohistochemistry (Paraffin) (IHC (P))

Myogenin Antibody in Immunoprecipitation (IP)

Myogenin Antibody in Immunohistochemistry (Frozen) (IHC (F))

Myogenin Antibody in Immunohistochemistry (IHC)

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Product Details

MA5-11486

Applications

Tested Dilution

Publications

Western Blot (WB)

1-2 µg/mL

View 1 publication 1 publication

Immunohistochemistry (IHC)

View 9 publications 9 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:400-1:800

Immunohistochemistry (Frozen) (IHC (F))

View 1 publication 1 publication

Immunocytochemistry (ICC/IF)

5 µg/mL

View 4 publications 4 publications

Immunoprecipitation (IP)

2 µg/mL

Gel Shift (GS)

1mg/mL

Product Specifications

Species Reactivity

Cat, Human, Mouse, Rat

Published species

Human, Mouse, Rat

Host/Isotype

Mouse / IgG1, kappa

Class

Monoclonal

Type

Antibody

Clone

F5D

Immunogen

Recombinant protein containing rat myogenin aa 30-224

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.5 mg/mL

Purification

Protein G

Storage buffer

PBS, pH 7.2

Contains

0.09% sodium azide

Storage conditions

4°C

Shipping conditions

Ambient (domestic); Wet ice (international)

AB_10977211

Product Specific Information

MA5-11486 targets Myogenin in GS, IF, IHC (P), IP, and WB applications and shows reactivity with Feline, Human, mouse, and Rat samples.

The MA5-11486 immunogen is recombinant protein containing rat myogenin aa 30-224.

Target Information

Myogenin is a muscle-specific transcription factor that can induce myogenesis in a variety of cell types in tissue culture. It is a member of a large family of proteins related by sequence homology, the helix-loop-helix proteins. It is essential for the development of functional skeletal muscle.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Traditional Chinese Medicine for Topical Treatment of Skeletal Muscle Injury.

Pharmaceuticals (Basel, Switzerland)

Siu WS,Ma H,Cheng W,Shum WT,Leung PC

MA5-11486 was used in Immunohistochemistry to demonstrate that topical Carthami Flos, Dipsaci Radix, and Rhei Rhizoma (CDR) treatment was effective at facilitating muscle injury repair.

Sat Aug 12 00:00:00 UTC 2023

Traditional Chinese Medicine for Topical Treatment of Skeletal Muscle Injury.

Pharmaceuticals (Basel, Switzerland)

Siu WS,Ma H,Cheng W,Shum WT,Leung PC

MA5-11486 was used in Immunohistochemistry to demonstrate that topical Carthami Flos, Dipsaci Radix, and Rhei Rhizoma (CDR) treatment was effective at facilitating muscle injury repair.

Sat Aug 12 00:00:00 UTC 2023

RNA-Seq analysis of a Pax3-expressing myoblast clone in-vitro and effect of culture surface stiffness on differentiation.

Scientific reports

Richardson L,Wang D,Hughes R,Johnson CA,Peckham M

Published figure using Myogenin monoclonal antibody (Product # MA5-11486) in Immunohistochemistry

Fri Feb 18 00:00:00 UTC 2022

Dysregulation of Muscle-Specific MicroRNAs as Common Pathogenic Feature Associated with Muscle Atrophy in ALS, SMA and SBMA: Evidence from Animal Models and Human Patients.

International journal of molecular sciences

Malacarne C,Galbiati M,Giagnorio E,Cavalcante P,Salerno F,Andreetta F,Cagnoli C,Taiana M,Nizzardo M,Corti S,Pensato V,Venerando A,Gellera C,Fenu S,Pareyson D,Masson R,Maggi L,Dalla Bella E,Lauria G,Mantegazza R,Bernasconi P,Poletti A,Bonanno S,Marcuzzo S

MA5-11486 was used in Western Blot to highlight the role of myomiRs as promising biomarkers in ALS, SMA, and SBMA.

Wed May 26 00:00:00 UTC 2021

Dysregulation of Muscle-Specific MicroRNAs as Common Pathogenic Feature Associated with Muscle Atrophy in ALS, SMA and SBMA: Evidence from Animal Models and Human Patients.

International journal of molecular sciences

MA5-11486 was used in Western Blot to highlight the role of myomiRs as promising biomarkers in ALS, SMA, and SBMA.

Wed May 26 00:00:00 UTC 2021

HSP70 drives myoblast fusion during C2C12 myogenic differentiation.

Biology open

Thakur SS,Swiderski K,Chhen VL,James JL,Cranna NJ,Islam AMT,Ryall JG,Lynch GS

MA5-11486 was used in Immunocytochemistry-immunoflourescence to reveal that increased HSP70 expression can promote myoblast fusion, identifying a mechanism for its therapeutic potential to enhance muscle repair after injury.

Wed Jul 22 00:00:00 UTC 2020

HSP70 drives myoblast fusion during C2C12 myogenic differentiation.

Biology open

Thakur SS,Swiderski K,Chhen VL,James JL,Cranna NJ,Islam AMT,Ryall JG,Lynch GS

Wed Jul 22 00:00:00 UTC 2020

PAX3 Confers Functional Heterogeneity in Skeletal Muscle Stem Cell Responses to Environmental Stress.

Cell stem cell

Der Vartanian A,Quétin M,Michineau S,Auradé F,Hayashi S,Dubois C,Rocancourt D,Drayton-Libotte B,Szegedi A,Buckingham M,Conway SJ,Gervais M,Relaix F

MA5-11486 was used in Immunohistochemistry to identify a functional heterogeneity of MuSCs in response to environmental stress controlled by PAX3.

Thu Jun 06 00:00:00 UTC 2019

Editing DNA Methylation in the Mammalian Genome.

Cell

Liu XS,Wu H,Ji X,Stelzer Y,Wu X,Czauderna S,Shu J,Dadon D,Young RA,Jaenisch R

MA5-11486 was used in Gel Shift, Immunocytochemistry, Immunohistochemistry (Paraffin), Immunoprecipitation, Western Blot to edit the methylation status of genomic sequences via the CRISPR/Cas9 system.

Thu Sep 22 00:00:00 UTC 2016

Recovery of fertility in azoospermia rats after injection of adipose-tissue-derived mesenchymal stem cells: the sperm generation.

BioMed research international

Cakici C,Buyrukcu B,Duruksu G,Haliloglu AH,Aksoy A,Isık A,Uludag O,Ustun H,Subası C,Karaoz E

MA5-11486 was used in immunohistochemistry to test if mesenchymal stem cells derived from adipose tissue can alleviate azoospermia in rats

Tue Nov 12 00:00:00 UTC 2013

A comprehensive characterization study of human bone marrow mscs with an emphasis on molecular and ultrastructural properties.

Journal of cellular physiology

Karaöz E,Okçu A,Gacar G,Sağlam O,Yürüker S,Kenar H

MA5-11486 was used in immunocytochemistry to study the molecular and ultrastructural properties of human bone marrow-derived mesenchymal stem cells

Sun May 01 00:00:00 UTC 2011

Mesenchymal stem cells improve the healing of ischemic colonic anastomoses (experimental study).

Langenbeck's archives of surgery

Adas G,Arikan S,Karatepe O,Kemik O,Ayhan S,Karaoz E,Kamali G,Eryasar B,Ustek D

MA5-11486 was used in immunohistochemistry to study the role of allogenic mesenchymal stem cell transplantation in improved healing of ischemic colon anastomosis

Sat Jan 01 00:00:00 UTC 2011

Molecular characterization of an EWSR1-POU5F1 fusion associated with a t(6;22) in an undifferentiated soft tissue sarcoma.

Cancer genetics

Deng FM,Galvan K,de la Roza G,Zhang S,Souid AK,Stein CK

MA5-11486 was used in immunohistochemistry to characterize a clinical case of undifferentiated soft tissue sarcoma with chromosomal rearrangement and resultant EWSR1-POU5F1 fusion

Mon Aug 01 00:00:00 UTC 2011

Endometriosis-associated skeletal muscle regeneration: a hitherto undescribed entity and a potential diagnostic pitfall.

The American journal of surgical pathology

Colella R,Mameli MG,Bellezza G,Sordo RD,Cavaliere A,Sidoni A

MA5-11486 was used in immunohistochemistry to investigate the association between endometriosis and skeletal muscle regeneration

Fri Jan 01 00:00:00 UTC 2010

Isolation and in vitro characterisation of dental pulp stem cells from natal teeth.

Histochemistry and cell biology

Karaöz E,Doğan BN,Aksoy A,Gacar G,Akyüz S,Ayhan S,Genç ZS,Yürüker S,Duruksu G,Demircan PC,Sariboyaci AE

MA5-11486 was used in immunocytochemistry to characterize the biological properties and differentiation potential of human natal dental pulp stem cells

Fri Jan 01 00:00:00 UTC 2010

Pulmonary meningothelial-like nodules: new insights into a common but poorly understood entity.

The American journal of surgical pathology

Mukhopadhyay S,El-Zammar OA,Katzenstein AL

MA5-11486 was used in immunohistochemistry to study pulmonary meningothelial-like nodules

Wed Apr 01 00:00:00 UTC 2009

Characterization of mesenchymal stem cells from rat bone marrow: ultrastructural properties, differentiation potential and immunophenotypic markers.

Histochemistry and cell biology

Karaoz E,Aksoy A,Ayhan S,Sariboyaci AE,Kaymaz F,Kasap M

MA5-11486 was used in immunocytochemistry to study the morphology and immunology of rat bone marrow-derived mesenchymal stem cells

Sun Nov 01 00:00:00 UTC 2009

Bioinformatics

Protein Aliases: bHLHc3; Class C basic helix-loop-helix protein 3; Myf-4; MYOD1-related protein; Myogenic factor 4; Myogenic factor-4; Myogenin

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Gene Aliases: BHLHC3; myf-4; MYF4; myo; MYOG

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UniProt ID: (Human) P15173, (Mouse) P12979, (Rat) P20428

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Entrez Gene ID: (Human) 4656, (Mouse) 17928, (Rat) 29148

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Function(s)

RNA polymerase II regulatory region sequence-specific DNA binding RNA polymerase II core promoter proximal region sequence-specific DNA binding core promoter binding transcriptional activator activity, RNA polymerase II core promoter proximal region sequence-specific binding transcriptional activator activity, RNA polymerase II transcription regulatory region sequence-specific binding transcription factor activity, sequence-specific DNA binding transcription factor activity, RNA polymerase II distal enhancer sequence-specific binding protein binding chromatin DNA binding sequence-specific DNA binding protein heterodimerization activity E-box binding core promoter proximal region sequence-specific DNA binding DNA binding protein dimerization activity molecular_function

Process(es)

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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Myogenin Monoclonal Antibody (F5D)

Myogenin Antibody (MA5-11486) in ICC/IF

Product Details

MA5-11486

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (Paraffin) (IHC (P))

Immunohistochemistry (Frozen) (IHC (F))

Immunocytochemistry (ICC/IF)

Immunoprecipitation (IP)

Gel Shift (GS)

Species Reactivity

Published species

Host/Isotype

Class

Type

Clone

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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