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Zeta
Myogenin Monoclonal Antibody (F5D)
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Product Details
Z2054MP
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Mouse
/ IgG1, kappa
Class
Monoclonal
Type
Antibody
Clone
F5D
Immunogen
Recombinant protein containing rat myogenin aa 30-224
Conjugate
Form
Liquid
Purification
Protein A
Storage buffer
tris with BSA, NP-40
Contains
<0.1% sodium azide
Storage conditions
4°C
Shipping conditions
Ambient (domestic); Wet ice (international)
Product Specific Information
This product is diluted and in a ready-to-use formulation.
A recommended positive control tissue for this product is Rhabdomyosarcoma, however positive controls are not limited to this tissue type.
The primary antibody is intended for laboratory professional use in the detection of the corresponding protein in formalin-fixed, paraffin-embedded tissue stained in manual qualitative immunohistochemistry (IHC) testing. This antibody is intended to be used after the primary diagnosis of tumor has been made by conventional histopathology using non-immunological histochemical stains.
Myogenin is a member of a family of myogenic regulatory genes, which includes MyoD, myf5 and MRF4. These genes encode a set of transcription factors, which are essential for muscle development. Transfection of myogenin into multipotential mesodermal cells have been shown to convert the mesodermal cells to myoblasts. Expression of myogenin is restricted to cells of skeletal muscle origin. It is therefore a useful marker for tumors of the muscle lineage, being strongly expressed in alveolar rhabdomyosarcomas.
Antibody is used with formalin-fixed and paraffin-embedded sections. Pretreatment of deparaffinized tissue with heat-induced epitope retrieval or enzymatic retrieval is recommended. In general, immunohistochemical (IHC) staining techniques allow for the visualization of antigens via the sequential application of a specific antibody to the antigen (primary antibody), a secondary antibody to the primary antibody (link antibody), an enzyme complex and a chromogenic substrate with interposed washing steps. The enzymatic activation of the chromogen results in a visible reaction product at the antigen site. Results are interpreted using a light microscope and aid in the differential diagnosis of pathophysiological processes, which may or may not be associated with a particular antigen.
A positive tissue control must be run with every staining procedure performed. This tissue may contain both positive and negative staining cells or tissue components and serve as both the positive and negative control tissue. External Positive control materials should be fresh autopsy/biopsy/surgical specimens fixed, processed and embedded as soon as possible in the same manner as the patient sample (s). Positive tissue controls are indicative of correctly prepared tissues and proper staining methods. The tissues used for the external positive control materials should be selected from the patient specimens with well-characterized low levels of the positive target activity that gives weak positive staining. The low level of positivity for external positive controls is designed to ensure detection of subtle changes in the primary antibody sensitivity from instability or problems with the staining methodology. A tissue with weak positive staining is more suitable for optimal quality control and for detecting minor levels of reagent degradation.
Internal or external negative control tissue may be used depending on the guidelines and policies that govern the organization to which the end user belongs to. The variety of cell types present in many tissue sections offers internal negative control sites, but this should be verified by the user. The components that do not stain should demonstrate the absence of specific staining, and provide an indication of non-specific background staining. If specific staining occurs in the negative tissue control sites, results with the patient specimens must be considered invalid.
Target Information
Myogenin is a muscle-specific transcription factor that can induce myogenesis in a variety of cell types in tissue culture. It is a member of a large family of proteins related by sequence homology, the helix-loop-helix proteins. It is essential for the development of functional skeletal muscle.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: bHLHc3; Class C basic helix-loop-helix protein 3; Myf-4; Myogenic factor 4; Myogenic factor-4; Myogenin
Gene Aliases: BHLHC3; myf-4; MYF4; MYOG
UniProt ID: (Human) P15173
Entrez Gene ID: (Human) 4656
RNA polymerase II regulatory region sequence-specific DNA binding
RNA polymerase II core promoter proximal region sequence-specific DNA binding
core promoter binding
transcriptional activator activity, RNA polymerase II core promoter proximal region sequence-specific binding
transcriptional activator activity, RNA polymerase II transcription regulatory region sequence-specific binding
transcription factor activity, sequence-specific DNA binding
transcription factor activity, RNA polymerase II distal enhancer sequence-specific binding
protein binding
chromatin DNA binding
sequence-specific DNA binding
protein heterodimerization activity
E-box binding
ossification
cell cycle
skeletal muscle tissue development
negative regulation of cell proliferation
response to gravity
positive regulation of myotube differentiation
skeletal muscle atrophy
positive regulation of muscle atrophy
regulation of skeletal muscle satellite cell proliferation
response to muscle activity involved in regulation of muscle adaptation
response to electrical stimulus involved in regulation of muscle adaptation
striated muscle atrophy
response to denervation involved in regulation of muscle adaptation
skeletal muscle cell differentiation
muscle cell fate commitment
mRNA transcription from RNA polymerase II promoter
skeletal muscle tissue regeneration
myoblast differentiation
positive regulation of myoblast differentiation
negative regulation of glycolytic process
positive regulation of transcription from RNA polymerase II promoter
skeletal muscle fiber development
positive regulation of skeletal muscle fiber development
positive regulation of muscle cell differentiation
positive regulation of cell cycle arrest
cellular response to magnetism
cellular response to lithium ion
cellular response to retinoic acid
cellular response to growth factor stimulus
cellular response to estradiol stimulus
regulation of myoblast fusion
positive regulation of myoblast fusion
positive regulation of oxidative phosphorylation
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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