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Rockland
Myosin-6 Polyclonal Antibody
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FIGURE: 1 / 1
Myosin-6 Antibody (100-401-J03) in WB
Western Blot of Rabbit anti-Myosin-6 antibody. Lane 1: SF9 cell lysate of truncated smooth myosin. Lane 2: Jurkat lysate (p/n W09-001-370). Lane 3: LnCap lysate (p/n W09-001-GJ9). Lane 4: Recombinant myosin VI. Load: 20µg per lane for cell lysate. 50ng of recombinant protein. Primary antibody: Myosin 6 antibody at 1:1000 for overnight at 4°C. Secondary antibody: HRP rabbit secondary antibody at 1:40,000 for 60 min at RT. Block: 5% BLOTTO overnight at 4°C. Predicted/Observed size: 150 kDa for Myo6.
Product Details
100-401-J03
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Pig
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Anti-Myosin-6 was prepared from whole rabbit serum produced by repeated immunizations with full length myosin-6 construct expressed in SF9 insect cells corresponding to porcine myosyn-6 protein.
Conjugate
Form
Liquid
Concentration
25 mg/mL
Storage buffer
0.02M potassium phosphate/whole serum, pH 7.2, with 0.15M NaCl
Contains
0.01% sodium azide
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
Product Specific Information
Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
Anti-Myosin-6 is directed against the myosin-6 protein. A BLAST analysis was used to suggest reactivity with 94% human Myosin VI. Cross-reactivity with myosin6 from other sources have not been determined.
Target Information
This gene encodes a protein involved intracellular vesicle and organelle transport, especially in the hair cell of the inner ear. Mutations in this gene have been found in patients with non-syndromic autosomal dominant and recessive hearing loss.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: myosin-VI; unconventional myosin; Unconventional myosin-6; Unconventional myosin-VI
Gene Aliases: DFNA22; DFNB37; KIAA0389; MYO6
UniProt ID: (Pig) Q29122, (Human) Q9UM54
Entrez Gene ID: (Pig) 397085, (Human) 4646
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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