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Rockland
Myosin Polyclonal Antibody
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FIGURE: 1 / 1
Myosin Antibody (600-401-938) in WB
Western blot using Rocklands anti-RLC of Smooth and Non-muscle Myosin antibody to detect vascular myosin (rat aorta, lane 1) but not cardiac myosin (mouse heart, lane2). Each lane was loaded with 35 µg of lysate. Arrowheads indicate the detection of both mono-phosphorylated (upper) and unphosphorylated (lower) forms of the protein. After blocking with 5% Normal goat serum and 0.5% BLOTTO in PBS, the membrane was probed with the primary antibody diluted in blocking buffer to 1:600 for 2 h at room temperature. The membrane was washed and r... View More
Product Details
600-401-938
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to amino acids aa 10-35 of human myosin light chain protein.
Conjugate
Form
Liquid
Concentration
0.98 mg/mL
Purification
Affinity chromatography
Storage buffer
0.02M potassium phosphate, pH 7.2, with 0.15M NaCl
Contains
0.01% sodium azide
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
Product Specific Information
Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
This affinity purified antibody is directed against the regulatory light chain of smooth and non-muscle myosin. The antibody detects both unphosphorylated and monophosphorylated forms of the protein. Cross reactivity is expected with myosin light chain from human, mouse and rat sources. Reactivity with the protein from other species has not been determined; however, the sequence of the immunogen is nearly identical in mammalian and avian species. BLAST search analysis was used to determine that the smooth and non-muscle forms of myosin regulatory light chain have identical sequences. Cross reactivity is expected.
Target Information
Myosin regulatory light polypeptide 9 is a highly conserved, ubiquitously expressed protein that in humans is encoded by the MYL9 gene. Myosin, a structural component of muscle, are hexameric proteins consisting of two heavy chains and four light chains, including a single pair of nonphosphorylatable light chain subunits and another pair of phosphorylatable light chain subunits.The protein encoded by this gene is a myosin light chain that may regulate muscle contraction by modulating the ATPase activity of myosin heads. Myosin light chain (Myl) 9 and Myl12 are new functional ligands for CD69. Blockade of CD69-Myl9/12 interactio ameliorates allergic airway inflammation in ovalbumin-induced and house dust mite-induced mouse models of asthma.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: 20 kDa myosin light chain; brain specific myosin regulatory light chain; Epididymis secretory protein Li 24; HEL-S-24; LC20; MLC-2; MLC-2A; MLC-2B; MLC-2C; MLC20; myosin light chain, regulatory B; myosin light chain, regulatory B-like; myosin light chain, regulatory C; myosin regulatory light chain 1; Myosin regulatory light chain 12A; Myosin regulatory light chain 12B; myosin regulatory light chain 2; Myosin regulatory light chain 2, nonsarcomeric; Myosin regulatory light chain 2, smooth muscle isoform; Myosin regulatory light chain 2-A, smooth muscle isoform; Myosin regulatory light chain 2-B, smooth muscle isoform; Myosin regulatory light chain 20 kDa; myosin regulatory light chain 3; Myosin regulatory light chain 9; Myosin regulatory light chain MRLC1; Myosin regulatory light chain MRLC2; Myosin regulatory light chain MRLC3; Myosin regulatory light chain RLC-A; myosin regulatory light chain-B; Myosin regulatory light polypeptide 9; Myosin RLC; Myosin RLC-A; Myosin RLC-B; myosin, light chain 12A, regulatory, non-sarcomeric; myosin, light chain 12B, regulatory; myosin, light chain 9, regulatory; myosin, light polypeptide 9, regulatory; myosin, light polypeptide, regulatory, non-sarcomeric (20kD); NMDA receptor-interacting protein; SHUJUN-1
Gene Aliases: 1500001M02Rik; 2900073G15Rik; AI327049; AI838292; C77744; C80200; HEL-S-24; LC20; MLC-2B; MLC-2C; MLC-B; MLC2; MLC20; MLCB; MRCL3; MRLC1; MRLC2; MRLC3; Mrlcb; MYL12A; MYL12B; MYL2B; MYL9; MYLC2B; Mylc2c; MYRL2; RGD1309537; RLC; Rlc-a; RLC-B; RLC-C
UniProt ID: (Human) P19105, (Human) O14950, (Human) P24844, (Rat) P13832, (Rat) P18666, (Mouse) Q3THE2, (Rat) Q64122, (Mouse) Q9CQ19
Entrez Gene ID: (Human) 10627, (Human) 103910, (Human) 10398, (Rat) 501203, (Mouse) 67268, (Rat) 50685, (Mouse) 67938, (Rat) 296313, (Mouse) 98932
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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