Invitrogen

Myosin Skeletal Muscle Polyclonal Antibody

Myosin Skeletal Muscle Antibody in Immunocytochemistry (ICC/IF)

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Myosin Skeletal Muscle Antibody (PA5-31466) in ICC/IF

Immunofluorescence analysis of Myosin Skeletal Muscle was performed using C2C12 and C2C12 cells differentiated to myotubes (2% Horse serum for 5 days). The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with Myosin Skeletal Muscle Rabbit Polyclonal Antibody (Product... View More

Myosin Skeletal Muscle Antibody in Western Blot (WB)

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Product Details

PA5-31466

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:3,000

View 3 publications 3 publications

Immunocytochemistry (ICC/IF)

5 µg/mL

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Mouse

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Recombinant protein encompassing a sequence within the N-terminus region of human MYH1. The exact sequence is proprietary.

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.18 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7, with 20% glycerol, 1% BSA

Contains

0.025% ProClin 300

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Ambient (domestic); Wet ice (international)

AB_2548939

Product Specific Information

Recommended positive controls: RD, mouse heart.

Predicted reactivity: Mouse (98%), Rat (97%), Xenopus laevis (81%), Dog (98%), Pig (99%), Rabbit (97%), Rhesus Monkey (100%), Bovine (99%).

Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

Target Information

Myosin is a major contractile protein which converts chemical energy into mechanical energy through the hydrolysis of ATP. Myosin is a hexameric protein composed of a pair of myosin heavy chains (MYH) and two pairs of nonidentical light chains. Myosin heavy chains are encoded by a multigene family. In mammals at least 10 different myosin heavy chain (MYH) isoforms have been described from striated, smooth, and nonmuscle cells. These isoforms show expression that is spatially and temporally regulated during development.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

LncRNA XLOC_015548 affects the proliferation and differentiation of myoblasts via the MAPK signaling pathway.

Experimental biology and medicine (Maywood, N.J.)

Wei Y,Qi T,Cao S,Zhang W,Yu F,Zeng H,Weng J

PA5-31466 was used in Immunocytochemistry to provide a laboratory foundation for the clinical application of lncRNAs as regulatory factors in the treatment of disuse muscle atrophy.

Wed Mar 01 00:00:00 UTC 2023

LncRNA XLOC_015548 affects the proliferation and differentiation of myoblasts via the MAPK signaling pathway.

Experimental biology and medicine (Maywood, N.J.)

Wei Y,Qi T,Cao S,Zhang W,Yu F,Zeng H,Weng J

PA5-31466 was used in Immunocytochemistry to provide a laboratory foundation for the clinical application of lncRNAs as regulatory factors in the treatment of disuse muscle atrophy.

Wed Mar 01 00:00:00 UTC 2023

Increased glucose metabolism in Arid5b^-/- skeletal muscle is associated with the down-regulation of TBC1 domain family member 1 (TBC1D1).

Biological research

Okazaki Y,Murray J,Ehsani A,Clark J,Whitson RH,Hirose L,Yanaka N,Itakura K

PA5-31466 was used in Western Blotting to demonstrate previously that AT-rich interactive domain 5b (Arid5b) knockout (Arid5b-/-) mice were lean and resistant to high-fat diet (HFD)-induced obesity.

Tue Oct 06 00:00:00 UTC 2020

Increased glucose metabolism in Arid5b^-/- skeletal muscle is associated with the down-regulation of TBC1 domain family member 1 (TBC1D1).

Biological research

Okazaki Y,Murray J,Ehsani A,Clark J,Whitson RH,Hirose L,Yanaka N,Itakura K

PA5-31466 was used in Western Blotting to demonstrate previously that AT-rich interactive domain 5b (Arid5b) knockout (Arid5b-/-) mice were lean and resistant to high-fat diet (HFD)-induced obesity.

Tue Oct 06 00:00:00 UTC 2020

Omega-3 Polyunsaturated Fatty Acids Mitigate Palmitate-Induced Impairments in Skeletal Muscle Cell Viability and Differentiation.

Frontiers in physiology

Tachtsis B,Whitfield J,Hawley JA,Hoffman NJ

PA5-31466 was used in Western Blotting to demonstrate the n-3 PUFAs EPA, DPA and DHA elicit similar protective effects against PAL-induced impairments in muscle cell viability and differentiation.

Mon Sep 28 00:00:00 UTC 2020

Bioinformatics

Protein Aliases: 2A myosin heavy chain; 2B myosin heavy chain; epididymis luminal protein 71; fast 2a myosin heavy chain; inclusion body myopathy 3, autosomal dominant; MGC133384; myh4; MyHC-2a; MyHC-2b; MyHC-2x; MyHC-IIa; MyHC-IIb; MyHC-IIx/d; Myosin heavy chain 1; Myosin heavy chain 2; Myosin heavy chain 2a; Myosin heavy chain 2b; Myosin heavy chain 2x; Myosin heavy chain 4; Myosin heavy chain IIa; Myosin heavy chain IIb; Myosin heavy chain IIx/d; myosin heavy chain type IIb; myosin heavy chain type IIx; Myosin heavy chain, skeletal muscle, adult 1; Myosin heavy chain, skeletal muscle, adult 2; Myosin heavy chain, skeletal muscle, fetal; myosin, heavy chain 1, skeletal muscle, adult; myosin, heavy chain 2, skeletal muscle, adult; myosin, heavy chain 4, skeletal muscle; myosin, heavy polypeptide 1, skeletal muscle, adult; myosin, heavy polypeptide 2, skeletal muscle, adult; myosin, heavy polypeptide 4, skeletal muscle; Myosin-1; Myosin-2; Myosin-4; skeletal muscle myosin heavy chain II-a; type 2X myosin heavy chain; type IIA myosin heavy chain

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Gene Aliases: A530084A17Rik; AI506973; HEL71; IBM3; IId; IId/x; MdMs; MHC-2X/D; MHC2A; MHC2B; MHC2X/D; Minimsc; Minmus; MM; MYH-2B; MYH1; MYH2; MYH2A; MYH2B; MYH4; MYHa; MYHas8; MYHC; MyHC-2A; MyHC-2B; MyHC-2x; MyHC-2X/D; MyHC-IIA; MyHC-IIb; MyHC-IId/x; MYHC-IIX; MyHC-IIx/d; Myhs-f; Myhs-f1; Myhs-f2; MYHSA1; MYHSA2; Myhsf; Myhsf1; Myhsf2; MYPOP

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UniProt ID: (Human) P12882, (Human) Q9UKX2, (Human) Q9Y623, (Mouse) Q5SX40, (Mouse) G3UW82, (Mouse) Q5SX39, (Rat) Q29RW1

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Entrez Gene ID: (Human) 4619, (Human) 4620, (Human) 4622, (Mouse) 17879, (Rat) 287408, (Rat) 691644, (Mouse) 17882, (Mouse) 17884, (Rat) 360543

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Function(s)

motor activity actin binding protein binding calmodulin binding ATP binding microfilament motor activity double-stranded RNA binding ATPase activity nucleotide binding molecular_function

Process(es)

plasma membrane repair muscle contraction metabolic process response to activity myosin filament assembly actin-mediated cell contraction actin filament-based movement muscle filament sliding ATP metabolic process Fc-gamma receptor signaling pathway involved in phagocytosis response to gravity response to muscle activity response to muscle stretch muscle structure development striated muscle contraction

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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Myosin Skeletal Muscle Polyclonal Antibody

Myosin Skeletal Muscle Antibody (PA5-31466) in ICC/IF

Product Details

PA5-31466

Western Blot (WB)

Immunocytochemistry (ICC/IF)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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