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Invitrogen
Myosin Skeletal Muscle Monoclonal Antibody (414)
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Myosin Skeletal Muscle Antibody (MA1-35718) in WB
Western blot was performed using Myosin Skeletal Muscle Monoclonal Antibody (414) (Product # MA1-35718) and a 223 kDa band corresponding to Myosin Skeletal Muscle was observed in RD, C2C12 differentiated to myotubes and L6 but not in HeLa, A549, MCF7 and PC12. Whole cell extracts (30 µg lysate) of RD (Lane 1), HeLa (Lane 2), A549 (Lane 3), MCF7 (Lane 4), C2C12 (Lane 5), C2C12 differentiated to myotubes (Lan... View More
Product Details
MA1-35718
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Chicken,
Human,
Mouse,
Rabbit,
Rat
Published species
Rat
Host/Isotype
Mouse
/ IgM
Class
Monoclonal
Type
Antibody
Clone
414
Immunogen
Chicken skeletal muscle cell preparation
Conjugate
Form
Liquid
Concentration
Conc. Not Determined
Storage buffer
tissue culture supernatant
Contains
0.09% sodium azide
Storage conditions
4°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2147020
Product Specific Information
Mab 414 reacts with myosin-containing elements in most cell types. Antigen localization: cytoplasm In Western blot experiments the antibody reacts with a 75kD part of the heavy chain (epitope location on a myosin-common sitechain), no reaction with light chains. It is not reactive with alpha-cardiac myosin. Positive control: muscle, myofibrils (chicken)
Target Information
Myosin is a major contractile protein which converts chemical energy into mechanical energy through the hydrolysis of ATP. Myosin is a hexameric protein composed of a pair of myosin heavy chains (MYH) and two pairs of nonidentical light chains. Myosin heavy chains are encoded by a multigene family. In mammals at least 10 different myosin heavy chain (MYH) isoforms have been described from striated, smooth, and nonmuscle cells. These isoforms show expression that is spatially and temporally regulated during development.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: 2A myosin heavy chain; 2B myosin heavy chain; alpha (cardiomyopathy; cardiac muscle; epididymis luminal protein 71; fast 2a myosin heavy chain; heavy chain 1; heavy chain 6; heavy polypeptide 1; hypertrophic 1); inclusion body myopathy 3, autosomal dominant; MGC133384; myh4; MyHC-2a; MyHC-2b; MyHC-2x; MyHC-IIa; MyHC-IIb; MyHC-IIx/d; Myosin heavy chain 1; Myosin heavy chain 2; Myosin heavy chain 2a; Myosin heavy chain 2b; Myosin heavy chain 2x; Myosin heavy chain 4; Myosin heavy chain IIa; Myosin heavy chain IIb; Myosin heavy chain IIx/d; myosin heavy chain isoform 2b; myosin heavy chain type IIb; myosin heavy chain type IIx; Myosin heavy chain, skeletal muscle; myosin heavy chain, skeletal muscle, adult; Myosin heavy chain, skeletal muscle, adult 1; Myosin heavy chain, skeletal muscle, adult 2; Myosin heavy chain, skeletal muscle, fetal; Myosin heavy chain, skeletal muscle, juvenile; myosin, heavy chain 1, skeletal muscle, adult; myosin, heavy chain 2, skeletal muscle, adult; myosin, heavy chain 4, skeletal muscle; myosin, heavy chain 6, cardiac muscle, alpha (cardiomyopathy, hypertrophic 1); myosin, heavy polypeptide 1, skeletal muscle, adult; myosin, heavy polypeptide 2, skeletal muscle, adult; myosin, heavy polypeptide 4, skeletal muscle; Myosin-1; Myosin-2; Myosin-4; skeletal muscle; skeletal muscle myosin heavy chain II-a; type 2X myosin heavy chain; type IIA myosin heavy chain
Gene Aliases: A530084A17Rik; adult; AI506973; HEL71; IBM3; IId; IId/x; MdMs; MHC-2X/D; MHC2A; MHC2B; MHC2X/D; Minimsc; Minmus; MM; MYH-2B; MYH1; MYH2; MYH2A; MYH2B; MYH4; MYH6; MYHa; MYHas8; MYHC; MyHC-2A; MyHC-2B; MyHC-2x; MyHC-2X/D; MyHC-IIA; MyHC-IIb; MyHC-IId/x; MYHC-IIX; MyHC-IIx/d; Myhs-f; Myhs-f1; Myhs-f2; MYHSA1; MYHSA2; Myhsf; Myhsf1; Myhsf2; myosin; MYPOP
plasma membrane repair
muscle contraction
metabolic process
response to activity
myosin filament assembly
actin-mediated cell contraction
response to gravity
response to muscle activity
response to muscle stretch
muscle structure development
striated muscle contraction
actin filament-based movement
muscle filament sliding
ATP metabolic process
Fc-gamma receptor signaling pathway involved in phagocytosis
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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