Invitrogen

N-Ras Polyclonal Antibody

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N-Ras Antibody (PA5-28861) in WB

Western Blot using N-Ras Polyclonal Antibody (Product # PA5-28861). Non-transfected (–) and transfected (+) 293T whole cell extracts (30 µg) were separated by 12% SDS-PAGE, and the membrane was blotted with N-Ras Polyclonal Antibody (Product # PA5-28861) diluted at 1:5,000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Product Image Gallery

N-Ras Antibody — Figure 2 DUF5 Vv is a Ras site-specific endopeptidase. ( a ) Coomassie-stained 18% SDS-polyacrylamide gel of anti-HA immunoprecipitated proteins from cells expressing HA-HRas treated for 24 h as indicated. Lower band (HRas*) was excised for peptide sequencing with HRas peptide coverage highlighted in yellow. ( b ) Same fractions probed by immunoblotting to detect the N terminus (anti-HA) and C terminus (isotype-specific antibody). ( c ) Lysates from cells expressing HA-tagged KRas, NRas or HRas probed by immunoblotting as indicated. ( d ) In-vitro cleavage of 10 muM rKRas to KRas* with 10 muM rDUF5 Vv (inset) or concentration indicated. Error bars indicate mean+-s.d. ( n =3). ( e ) In-vitro cleavage of 10 muM rKRas, rHRas and rNRas with 10 muM rDUF5 Vv . Identical results of Edman degradation were obtained for all three proteins. ( f ). Black arrow indicates the cleavage site in the Switch I region (red) of HRas 69 . {TM}

Product Details

PA5-28861

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:3,000

View 2 publications 2 publications

Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Human

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Full length human N-Ras Recombinant protein.

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.41 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7, with 1% BSA, 20% glycerol

Contains

0.025% ProClin 300

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Ambient (domestic); Wet ice (international)

AB_2546337

Product Specific Information

Recommended positive controls: mouse brain.

Predicted reactivity: Mouse (99%), Rat (99%), Zebrafish (90%), Xenopus laevis (92%), Dog (100%), Pig (100%), Chicken (96%), Bovine (100%), Guinea pig (99%).

Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

Target Information

NRAS (GTPase NRas) is a membrane protein that shuttles between the Golgi apparatus and the plasma membrane. This shuttling is regulated through palmitoylation and depalmitoylation by the ZDHHC9-GOLGA7 complex. This protein, which has intrinsic GTPase activity, is activated to a GTP-bound form by a GTPase activating protein and inactivated to a GDP-bound form by a guanine nucleotide-exchange factor. Defects in the gene encoding this protein are a cause of juvenile myelomonocytic leukemia (JMML) and Noonan syndrome 6.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Integrated analysis of patient samples identifies biomarkers for venetoclax efficacy and combination strategies in acute myeloid leukemia.

Nature cancer

Zhang H,Nakauchi Y,Köhnke T,Stafford M,Bottomly D,Thomas R,Wilmot B,McWeeney SK,Majeti R,Tyner JW

PA5-28861 was used in Western Blot to show that Venetoclax in combination with an MCL1 inhibitor AZD5991 induced synthetic lethality and circumvented venetoclax resistance.

Sat Aug 01 00:00:00 UTC 2020

Site-specific processing of Ras and Rap1 Switch I by a MARTX toxin effector domain.

Nature communications

Antic I,Biancucci M,Zhu Y,Gius DR,Satchell KJF

PA5-28861 was used in western blot to characterize a new Ras/Rap1-specific endopeptidase.

Mon Jun 08 00:00:00 UTC 2015

Bioinformatics

Protein Aliases: GTPase NRas; N-ras protein part 4; Neuroblastoma RAS; neuroblastoma RAS viral (v-ras) oncogene homolog; RAS; RASN; Transforming protein N-Ras; v-ras neuroblastoma RAS viral oncogene homolog

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Gene Aliases: ALPS4; AV095280; CMNS; HRAS1; N-ras; NCMS; NRAS; NRAS1; NS6

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UniProt ID: (Human) P01111, (Rat) Q04970

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Entrez Gene ID: (Human) 4893, (Mouse) 18176, (Rat) 24605

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Function(s)

GTP binding protein complex binding nucleotide binding GTPase activity molecular_function protein binding

Process(es)

MAPK cascade stimulatory C-type lectin receptor signaling pathway epidermal growth factor receptor signaling pathway Ras protein signal transduction axon guidance Fc-epsilon receptor signaling pathway ERBB2 signaling pathway leukocyte migration establishment of protein localization to Golgi regulation of protein phosphorylation signal transduction small GTPase mediated signal transduction regulation of gene expression positive regulation of fibroblast proliferation negative regulation of skeletal muscle tissue development regulation of cell cycle biological_process positive regulation of cell proliferation visual learning actin cytoskeleton organization regulation of synaptic transmission, GABAergic positive regulation of Rac protein signal transduction negative regulation of neuron apoptotic process negative regulation of cell differentiation regulation of long-term neuronal synaptic plasticity striated muscle cell differentiation epithelial tube branching involved in lung morphogenesis

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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N-Ras Polyclonal Antibody

N-Ras Antibody (PA5-28861) in WB

Product Details

PA5-28861

Western Blot (WB)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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