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Invitrogen
N-Ras Recombinant Rabbit Monoclonal Antibody (10H6L11)
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FIGURE: 1 / 6
N-Ras Antibody (703435) in ICC/IF
For immunofluorescence analysis, MCF-7 cells were fixed and permeabilized using ice cold 100% Acetone for detection of endogenous N-Ras using Anti-N-Ras Recombinant Rabbit Monoclonal Antibody (Product # 703435, 1:100 dilution) and labeled with Goat anti-Rabbit IgG (Heavy Chain) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A27034, 1:2000). Panel a) shows representative cells that were stained for detection and localization of N-Ras protein (green), Panel b) is stained for nuclei (blue) using ProLong™ Diamond Antifa... View More
Product Details
703435
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Published species
Human
Host/Isotype
Rabbit
/ IgG
Expression System
Expi293
Class
Recombinant Monoclonal
Type
Antibody
Clone
10H6L11
Immunogen
Peptides corresponding to human N-Ras [1) aa83-aa98 2) aa166-aa180]
Conjugate
Form
Liquid
Concentration
0.5 mg/mL
Purification
Protein A
Storage buffer
PBS, pH 7.4
Contains
0.09% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Wet ice
RRID
AB_2784586
Product Specific Information
This antibody is against aa83-98 (AINNSKSFADINLYRE) as confirmed in ELISA testing.
This antibody is predicted to react with Pig, Rat, Rabbit.
Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The expression systems are developed by cloning in the specific antibody DNA sequences from immunoreactive rabbits. Then, individual clones are screened to select the best candidates for production. The advantages of using recombinant rabbit monoclonal antibodies include: better specificity and sensitivity, lot-to-lot consistency, animal origin-free formulations, and broader immunoreactivity to diverse targets due to larger rabbit immune repertoire.
Target Information
NRAS (GTPase NRas) is a membrane protein that shuttles between the Golgi apparatus and the plasma membrane. This shuttling is regulated through palmitoylation and depalmitoylation by the ZDHHC9-GOLGA7 complex. This protein, which has intrinsic GTPase activity, is activated to a GTP-bound form by a GTPase activating protein and inactivated to a GDP-bound form by a guanine nucleotide-exchange factor. Defects in the gene encoding this protein are a cause of juvenile myelomonocytic leukemia (JMML) and Noonan syndrome 6.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: GTPase NRas; N-ras protein part 4; Neuroblastoma RAS; neuroblastoma RAS viral (v-ras) oncogene homolog; RAS; RASN; Transforming protein N-Ras; v-ras neuroblastoma RAS viral oncogene homolog
Gene Aliases: ALPS4; CMNS; HRAS1; N-ras; NCMS; NRAS; NRAS1; NS6
UniProt ID: (Human) P01111
Entrez Gene ID: (Human) 4893
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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