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Invitrogen
N-WASP Monoclonal Antibody (C.427.3)
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FIGURE: 1 / 5
N-WASP Antibody (MA5-15011) in WB
Western blot was performed using N-WASP Monoclonal Antibody (C.427.3) (Product # MA5-15011) and a~60 kDa band corresponding to WASL was observed in SkBr3, SHSY5Y, A431, MCF7 Mouse Lung and Mouse Cerebellum; and was absent in HEL 92.1.7, HL60 and Mouse skeletal Muscle. Whole cell extracts (30 µg lysate) of SkBr3 (Lane 1), SHSY5Y (Lane 2), A431 (Lane 3), MCF7 (Lane 4), HEL92.1.7 (Lane 5), HL60 (Lane 6), tissue extracts (30 µg lysate) of Mouse Lung (Lane 7), Mouse Cerebellum (Lane 8) and Mouse Skeletal Muscle (Lane 9) were electrophoresed usi... View More
Product Details
MA5-15011
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Bovine,
Hamster,
Human,
Mouse,
Non-human primate,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Monoclonal
Type
Antibody
Clone
C.427.3
Immunogen
Synthetic peptide corresponding the sequence of human N-WASP
Conjugate
Form
Liquid
Purification
Affinity chromatography
Storage buffer
0.01M HEPES, pH 7.5, with 0.15M NaCl, 100µg/mL BSA, 50% glycerol
Contains
<0.02% sodium azide
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_10984648
Product Specific Information
It is not recommended to aliquot this antibody.
Target Information
The Wiskott-Aldrich syndrome (WAS) family of proteins share similar domain structure, and are involved in transduction of signals from receptors on the cell surface to the actin cytoskeleton. The presence of a number of different motifs suggests that they are regulated by a number of different stimuli, and interact with multiple proteins. Recent studies have demonstrated that these proteins, directly or indirectly, associate with the small GTPase, Cdc42, known to regulate formation of actin filaments, and the cytoskeletal organizing complex, Arp2/3. The WASL gene product is a homolog of WAS protein, however, unlike the latter, it is ubiquitously expressed and shows highest expression in neural tissues. It has been shown to bind Cdc42 directly, and induce formation of long actin microspikes.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Actin nucleation-promoting factor WASL; DKFZp779G0847; MGC48327; n wasp; N-WASP; Neural Wiskott-Aldrich syndrome protein; Wiskott-Aldrich syndrome-like
Gene Aliases: 2900021I12Rik; 3110031I02Rik; BOS_4408; N-WASP; NWASP; TRS4; WASL; WASPB
UniProt ID: (Bovine) Q95107, (Human) O00401, (Mouse) Q91YD9
Entrez Gene ID: (Bovine) 281577, (Human) 8976, (Chinese hamster) 100757697, (Mouse) 73178, (Rat) 682507
transcription, DNA-templated
regulation of transcription, DNA-templated
actin filament organization
response to bacterium
regulation of protein localization
cellular protein complex localization
spindle localization
membrane budding
cell cycle
mitotic nuclear division
membrane invagination
vesicle organization
cell projection assembly
actin cytoskeleton organization
vesicle transport along actin filament
actin nucleation
cell division
positive regulation of filopodium assembly
membrane tubulation
positive regulation of clathrin-mediated endocytosis
positive regulation of Arp2/3 complex-mediated actin nucleation
negative regulation of membrane tubulation
protein complex assembly
movement of cell or subcellular component
actin polymerization or depolymerization
Fc-gamma receptor signaling pathway involved in phagocytosis
ephrin receptor signaling pathway
regulation of nitric-oxide synthase activity
negative regulation of lymphocyte migration
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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