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          Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.

          • Primary Antibodies ›
          • N-cadherin Antibodies

          Invitrogen

          N-cadherin Monoclonal Antibody (3B9)

          Advanced Verification
          196 References
          View all (95) N-cadherin antibodies
          Datasheet
          Protocols
          Questions & Answers
          Datasheet
          Protocols
          Questions & Answers

          Cite N-cadherin Monoclonal Antibody (3B9)

          • Antibody Testing Data (6)
          • Advanced Verification (2)
          N-cadherin Antibody in Immunocytochemistry (ICC/IF)
          Group 53 Created with Sketch.
          N-cadherin Antibody in Immunocytochemistry (ICC/IF)
          Group 53 Created with Sketch.

          FIGURE: 1 / 8

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          N-cadherin Antibody (33-3900) in ICC/IF

          Immunofluorescent analysis of N-Cadherin was done on 70% confluent log phase Caco-2 cells. The cells were fixed with 4% paraformaldehyde for 15 minutes; permeabilized with 0.25% Triton™ X-100 for 10 minutes followed by blocking with 5% BSA for 1 hour at room temperature. The cells were incubated with N-Cadherin Mouse Monoclonal Antibody (Product # 33-3900) at 1 µg - 2 µg in 1% BSA and incubated for 3 hours ... View More {{ $ctrl.currentElement.advancedVerification.fullName }} validation info. View more
          Published figure supplied by benchsci-logo
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          View Product

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          N-cadherin Antibody in Immunocytochemistry (ICC/IF)
          N-cadherin Antibody in Immunocytochemistry (ICC/IF)
          N-cadherin Antibody in Immunohistochemistry (IHC)
          N-cadherin Antibody in Western Blot (WB)
          N-cadherin Antibody in Western Blot (WB)
          N-cadherin Antibody in Western Blot (WB)
          N-cadherin Antibody
          N-cadherin Antibody

          Product Details

          33-3900

          Applications
          Tested Dilution
          Publications

          Western Blot (WB)

          1:500-1:1,000
          View 65 publications 65 publications

          Immunohistochemistry (IHC)

          -
          View 48 publications 48 publications

          Immunohistochemistry (Paraffin) (IHC (P))

          1-5 µg/mL
          View 26 publications 26 publications

          Immunohistochemistry (Frozen) (IHC (F))

          -
          View 8 publications 8 publications

          Immunocytochemistry (ICC/IF)

          1-3 µg/mL
          View 28 publications 28 publications

          Flow Cytometry (Flow)

          -
          View 2 publications 2 publications

          Immunoprecipitation (IP)

          3-5 µg
          View 1 publication 1 publication

          Miscellaneous PubMed (MISC)

          -
          View 18 publications 18 publications
          Product Specifications

          Species Reactivity

          Chicken, Human, Mouse, Pig, Rat

          Published species

          Avian, Dog, Hamster, Horse, Human, Mouse, Non-human primate, Pig, Rabbit, Rat, Rhesus monkey, Tag, Zebrafish

          Host/Isotype

          Mouse / IgG1, kappa

          Class

          Monoclonal

          Type

          Antibody

          Clone

          3B9

          Immunogen

          Recombinant domain corresponding to the intracellular domain of chicken N-Cadherin

          Conjugate

          Unconjugated Unconjugated Unconjugated

          Form

          Liquid

          Concentration

          0.5 mg/mL

          Purification

          Protein A

          Storage buffer

          PBS, pH 7.4

          Contains

          0.1% sodium azide

          Storage conditions

          -20°C

          Shipping conditions

          Ambient (domestic); Wet ice (international)

          RRID

          AB_2313779

          Product Specific Information

          This antibody is specific for N-cadherin and does not cross-react with other cadherin family members including P- and E-cadherins.

          For IHC with this antibody use formalin-fixed, paraffin-embedded tissue and heat-induced epitope retrieval.

          Target Information

          N-cadherin is a 140 kDa protein belonging to a family of transmembrane molecules that mediate calcium-dependent intercellular adhesion. Cadherins are involved in controlling morphogenetic movements during development and regulate cell surface adhesion through homotypic adhesion with the same cadherin species. N-cadherin's function is dependent on its association with the actin-cytoskeleton and is mediated through interactions between the C-terminal region of N-cadherin and the cytoplasmic catenin proteins. The stability of this association is regulated by phosphorylation and dephosphorylation of beta-catenin. Further, N-cadherin is a calcium dependent cell-cell adhesion glycoprotein comprised of five extracellular cadherin repeats, a transmembrane region and a highly conserved cytoplasmic tail. The protein functions during gastrulation and is required for establishment of left-right asymmetry.

          For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

          Bioinformatics

          Protein Aliases: cadherin 2 type 1 N-cadherin (neuronal); cadherin 2, type 1, N-cadherin (neuronal); Cadherin-2; Cadherin-N; Cadherin2; calcium-dependent adhesion protein, neuronal; CD325; CDw325; CDw325 antigen; N-cadherin; N-cadherin 1; Neural cadherin

          View more View less

          Gene Aliases: CD325; CDH2; CDHN; CDw325; N-cadherin; NCAD

          View more View less

          UniProt ID: (Human) P19022, (Chicken) P10288, (Mouse) P15116, (Rat) Q9Z1Y3

          View more View less

          Entrez Gene ID: (Human) 1000, (Chicken) 414745, (Mouse) 12558, (Rat) 83501

          View more View less

          Function(s)
          calcium ion binding protein binding beta-catenin binding protein kinase binding protein phosphatase binding protein complex binding alpha-catenin binding gamma-catenin binding nitric-oxide synthase binding extracellular matrix binding enzyme binding metal ion binding
          Process(es)
          cell adhesion homophilic cell adhesion via plasma membrane adhesion molecules heterophilic cell-cell adhesion via plasma membrane cell adhesion molecules synapse assembly regulation of signal transduction glial cell differentiation single organismal cell-cell adhesion calcium-dependent cell-cell adhesion via plasma membrane cell adhesion molecules cell migration regulation of myelination regulation of protein localization regulation of Rho protein signal transduction positive regulation of MAPK cascade cell-cell adhesion mediated by cadherin blood vessel morphogenesis regulation of axonogenesis striated muscle cell differentiation protein heterooligomerization negative regulation of canonical Wnt signaling pathway neuroligin clustering involved in postsynaptic membrane assembly neuronal stem cell population maintenance regulation of postsynaptic density protein 95 clustering positive regulation of synaptic vesicle clustering cerebral cortex development adherens junction organization brain morphogenesis homeostasis of number of cells positive regulation of muscle cell differentiation radial glial cell differentiation neuroepithelial cell differentiation regulation of oligodendrocyte progenitor proliferation establishment of protein localization to plasma membrane cartilage condensation limb development
          It has to be done as per old AB suggested Products section.
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          If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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