Invitrogen

N-cadherin Monoclonal Antibody (NCD-2)

21 Published Figures

27 References

View all (95) N-cadherin antibodies

Datasheet

Protocols

Questions & Answers

Datasheet

Protocols

Questions & Answers

Antibody Testing Data (1)
Published Figures (21)

N-cadherin Antibody in Immunohistochemistry (IHC)

FIGURE: 1 / 22

N-cadherin Antibody (13-2100) in IHC

Immunofluorescent staining of the developing chick neural tube using N-Cadherin Monoclonal Antibody, Rat (Product # 13-2100).

N-cadherin Antibody in Western Blot (WB)

N-cadherin Antibody in Immunocytochemistry (ICC/IF)

N-cadherin Antibody in Western Blot, Immunocytochemistry (WB, ICC/IF)

N-cadherin Antibody in Western Blot, Immunohistochemistry (WB, IHC)

N-cadherin Antibody in Immunohistochemistry (PFA fixed) (IHC (PFA))

N-cadherin Antibody in Western Blot, Immunohistochemistry (PFA fixed) (WB, IHC (PFA))

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Product Details

13-2100

Applications

Tested Dilution

Publications

Western Blot (WB)

1-10 µg/mL

View 9 publications 9 publications

Immunohistochemistry (IHC)

View 6 publications 6 publications

Immunohistochemistry (Paraffin) (IHC (P))

View 1 publication 1 publication

Immunohistochemistry (PFA fixed) (IHC (PFA))

View 1 publication 1 publication

Immunohistochemistry (Frozen) (IHC (F))

10 µg/mL

Immunocytochemistry (ICC/IF)

View 5 publications 5 publications

Flow Cytometry (Flow)

View 1 publication 1 publication

Neutralization (Neu)

View 1 publication 1 publication

Inhibition Assays (IA)

100 µg/mL

Affinity Purification (AP)

View 1 publication 1 publication

Miscellaneous PubMed (MISC)

View 2 publications 2 publications

Product Specifications

Species Reactivity

Chicken, Xenopus

Published species

Avian, Chicken, Hamster, Human, Mouse, Xenopus

Host/Isotype

Rat / IgG2a

Class

Monoclonal

Type

Antibody

Clone

NCD-2

Immunogen

Neuronal tissue of chick embryos.

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Lyophilized

Concentration

2.0 mg/mL

Purification

purified

Storage buffer

PBS, pH 7.4, with 1% BSA

Contains

no preservative

Storage conditions

4°C

Shipping conditions

Ambient (domestic); Wet ice (international)

AB_2533007

Product Specific Information

Source: Monoclonal antibody was obtained by fusing the mouse myeloma cell-line P3-X63-Ag8-U1 with spleen cells of Wistar rat after immunization with nervous tissue of chick embryos. The monoclonal antibody was harvested from ascitic fluid of SCID mouse.

Specificity: This antibody specifically reacts with chicken N-cadherin. This antibody inhibits N-cadherin dependent cell-cell contact.

Cross reactivity: This antibody reacts with Xenopus N-cadherin. This antibody does not react with human or mouse N-cadherin.

Storage: 4°C This product does not contain preservative. The stock solution(2.0 mg/mL) should be stored in aliquots at -20°C for 1 year, or should be stored at 4°C for 6 months after adding 0.1% sodium azide. Avoid repeated freeze-thaw cycles.

Reconstitution: Dissolve the lyophilized antibody in 50 µL of distilled water (final concentration: 2.0 mg/mL). This solution can be used as a stock solution. If dilution is necessary for your application, dilute the stock solution with the following Dilution solution just prior to use. When the entire amount of antibody is to be used over a short time period, it may be dissolved directly in 500 µL or more of the Dilution solution. Be sure to store the antibody at a minimum concentration of 2.0 mg/mL. A lower antibody concentration may result in decreased stability. Reconstituted antibody solution should contain 0.1% sodium azide as a preservative when stored at 4°C.

Ca2+ should be contained in the buffers to stabilize cadherin antigen.

Target Information

N-cadherin is a 140 kDa protein belonging to a family of transmembrane molecules that mediate calcium-dependent intercellular adhesion. Cadherins are involved in controlling morphogenetic movements during development and regulate cell surface adhesion through homotypic adhesion with the same cadherin species. N-cadherin's function is dependent on its association with the actin-cytoskeleton and is mediated through interactions between the C-terminal region of N-cadherin and the cytoplasmic catenin proteins. The stability of this association is regulated by phosphorylation and dephosphorylation of beta-catenin. Further, N-cadherin is a calcium dependent cell-cell adhesion glycoprotein comprised of five extracellular cadherin repeats, a transmembrane region and a highly conserved cytoplasmic tail. The protein functions during gastrulation and is required for establishment of left-right asymmetry.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Vinculin is required for interkinetic nuclear migration (INM) and cell cycle progression.

The Journal of cell biology

Ochoa A,Herrera A,Menendez A,Estefanell M,Ramos C,Pons S

13-2100 was used in Western Blot to demonstrate that β-catenin targets vinculin to the apical AJs and the centrosome in the embryonic neural tube (NT).

Mon Jan 01 00:00:00 UTC 2024

Bioinformatics and Experimental Analyses Reveal MAP4K4 as a Potential Marker for Gastric Cancer.

Genes

Zhang J,Cai X,Cui W,Wei Z

Published figure using N-cadherin monoclonal antibody (Product # 13-2100) in Western Blot

Mon Oct 03 00:00:00 UTC 2022

Dbnl and β-catenin promote pro-N-cadherin processing to maintain apico-basal polarity.

The Journal of cell biology

Herrera A,Menendez A,Torroba B,Ochoa A,Pons S

13-2100 was used in Immunocytochemistry, Western Blot, Immunohistochemistry (PFA fixed) to demonstrate that Dbnl and Drebrin-like β-catenin assist in the maturation of pro-N-cadherin, which is critical for AJ formation and for the recruitment AC components like aPKC and, consequently, for the maintenance of apico-basal polarity.

Mon Jun 07 00:00:00 UTC 2021

Dbnl and β-catenin promote pro-N-cadherin processing to maintain apico-basal polarity.

The Journal of cell biology

Herrera A,Menendez A,Torroba B,Ochoa A,Pons S

Mon Jun 07 00:00:00 UTC 2021

Dbnl and β-catenin promote pro-N-cadherin processing to maintain apico-basal polarity.

The Journal of cell biology

Herrera A,Menendez A,Torroba B,Ochoa A,Pons S

Mon Jun 07 00:00:00 UTC 2021

Dbnl and β-catenin promote pro-N-cadherin processing to maintain apico-basal polarity.

The Journal of cell biology

Herrera A,Menendez A,Torroba B,Ochoa A,Pons S

Mon Jun 07 00:00:00 UTC 2021

Dbnl and β-catenin promote pro-N-cadherin processing to maintain apico-basal polarity.

The Journal of cell biology

Herrera A,Menendez A,Torroba B,Ochoa A,Pons S

Mon Jun 07 00:00:00 UTC 2021

Low-Dose Albendazole Inhibits Epithelial-Mesenchymal Transition of Melanoma Cells by Enhancing Phosphorylated GSK-3β/Tyr216 Accumulation.

Journal of oncology

He Z,Lei S,Liang F,Tan L,Zhang W,Xie L,Zheng H,Lu Y

Published figure using N-cadherin monoclonal antibody (Product # 13-2100) in Western Blot

Fri Dec 31 00:00:00 UTC 2021

Redistribution of Adhesive Forces through Src/FAK Drives Contact Inhibition of Locomotion in Neural Crest.

Developmental cell

Roycroft A,Szabó A,Bahm I,Daly L,Charras G,Parsons M,Mayor R

13-2100 was used in In vivo experiments to study the exact role of traction through cell-matrix adhesions, and tension through cell-cell adhesions during contact inhibition of locomotion.

Mon Jun 04 00:00:00 UTC 2018

Asymmetric localization of DLC1 defines avian trunk neural crest polarity for directional delamination and migration.

Nature communications

Liu JA,Rao Y,Cheung MPL,Hui MN,Wu MH,Chan LK,Ng IO,Niu B,Cheah KSE,Sharma R,Hodgson L,Cheung M

Published figure using N-cadherin monoclonal antibody (Product # 13-2100) in Immunofluorescence

Mon Oct 30 00:00:00 UTC 2017

Sustained Wnt/β-catenin signalling causes neuroepithelial aberrations through the accumulation of aPKC at the apical pole.

Nature communications

Herrera A,Saade M,Menendez A,Marti E,Pons S

13-2100 was used in immunohistochemistry and western blot to report that beta-catenin controls the cell fate and polarity of neuroblasts by modulating the expression and localization of aPKC.

Thu Jun 19 00:00:00 UTC 2014

Sustained Wnt/β-catenin signalling causes neuroepithelial aberrations through the accumulation of aPKC at the apical pole.

Nature communications

Herrera A,Saade M,Menendez A,Marti E,Pons S

13-2100 was used in immunohistochemistry and western blot to report that beta-catenin controls the cell fate and polarity of neuroblasts by modulating the expression and localization of aPKC.

Thu Jun 19 00:00:00 UTC 2014

Cell surface localization of α3β4 nicotinic acetylcholine receptors is regulated by N-cadherin homotypic binding and actomyosin contractility.

PloS one

Brusés JL

13-2100 was used in immunocytochemistry and western blot to investigate mechanisms that regulate the localization of nicotinic receptors.

Tue Nov 26 00:00:00 UTC 2013

Cell surface localization of α3β4 nicotinic acetylcholine receptors is regulated by N-cadherin homotypic binding and actomyosin contractility.

PloS one

Brusés JL

13-2100 was used in immunocytochemistry and western blot to investigate mechanisms that regulate the localization of nicotinic receptors.

Tue Nov 26 00:00:00 UTC 2013

Cell surface localization of α3β4 nicotinic acetylcholine receptors is regulated by N-cadherin homotypic binding and actomyosin contractility.

PloS one

Brusés JL

13-2100 was used in immunocytochemistry and western blot to investigate mechanisms that regulate the localization of nicotinic receptors.

Tue Nov 26 00:00:00 UTC 2013

Cell surface localization of α3β4 nicotinic acetylcholine receptors is regulated by N-cadherin homotypic binding and actomyosin contractility.

PloS one

Brusés JL

13-2100 was used in immunocytochemistry and western blot to investigate mechanisms that regulate the localization of nicotinic receptors.

Tue Nov 26 00:00:00 UTC 2013

Cell surface localization of α3β4 nicotinic acetylcholine receptors is regulated by N-cadherin homotypic binding and actomyosin contractility.

PloS one

Brusés JL

13-2100 was used in immunocytochemistry and western blot to investigate mechanisms that regulate the localization of nicotinic receptors.

Tue Nov 26 00:00:00 UTC 2013

N-cadherin modulates voltage activated calcium influx via RhoA, p120-catenin, and myosin-actin interaction.