Invitrogen

N-terminal Arginylation Monoclonal Antibody (4A9)

N-terminal Arginylation Antibody in Western Blot (WB)

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N-terminal Arginylation Antibody (MA5-27563) in WB

Western blot analysis of N-terminal Arginylation in n-terminal arginine-bsa using 0.5 µg of protein. Sample was prepared with 5% milk and TBST, incubated with N-terminal Arginylation monoclonal antibody (Product # MA5-27563) using a dilution of 1:1000 (1 hour at RT) and followed by a Goat Anti-Mouse PE secondary antibody at a dilution of 1:100 (20 min at RT). Samples are arranged as follows: Lane 1: molecul... View More

N-terminal Arginylation Antibody in Flow Cytometry (Flow)

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Product Details

MA5-27563

Applications

Tested Dilution

Publications

Western Blot (WB)

1:1,000

Flow Cytometry (Flow)

1:50

Product Specifications

Species Reactivity

Chemical

Host/Isotype

Mouse / IgG1

Class

Monoclonal

Type

Antibody

Clone

4A9

Immunogen

Synthetic N-terminal arginylated peptide conjugated to KLH

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Protein G

Storage buffer

PBS, pH 7.4, with 50% glycerol

Contains

0.09% sodium azide

Storage conditions

-20°C

Shipping conditions

Ambient (domestic); Wet ice (international)

AB_2735284

Product Specific Information

MA5-27563 is specific for N-terminal arginine next to glutamic acid. It does not detect N-terminal arginine next to aspartic acid or internal arginine residues.

A 1:1,000 dilution of MA5-27563 was sufficient for detection of N-terminal Arginylation in 0.5 µg of N-terminal Arginine peptide conjugated to BSA by ECL immunoblot analysis using goat anti-mouse IgG:HRP as the secondary antibody.

Specific for N-terminal arginine next to glutamic acid. Does not detect N-terminal arginine next to aspartic acid or internal arginine residues.

Target Information

Protein arginylation is the post-translational addition of arginine to proteins by arginyltransferase ATE1. Arginylation of proteins has been found to play an essential role in physiological pathways during embryogenesis and adulthood. Arginylation has also been shown to regulate cell stress responses, including ER stress, cytosolic misfolded proteins, and heat stress.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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