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Invitrogen
N6AMT2 Polyclonal Antibody
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N6AMT2 Antibody (PA5-110010) in WB
Western blot analysis of N6AMT2 in extracts of various cell lines. Samples were incubated with N6AMT2 Polyclonal antibody (Product # PA5-110010) using a dilution of 1:1,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.
Product Details
PA5-110010
Product Specifications
Species Reactivity
Human
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 1-214 of human N6AMT2 (NP_777588.1).
Conjugate
Form
Liquid
Concentration
0.67 mg/mL
Purification
Affinity Chromatography
Storage buffer
PBS, pH 7.3, with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Wet ice
RRID
AB_2855421
Product Specific Information
Immunogen sequence: MSDLEDDETP QLSAHALAAL QEFYAEQKQQ IEPGEDDKYN IGIIEENWQL SQFWYSQETA LQLAQEAIAA VGEGGRIACV SAPSVYQKLR ELCRENFSIY IFEYDKRFAM YGEEFIFYDY NNPLDLPERI AAHSFDIVIA DPPYLSEECL RKTSETVKYL TRGKILLCTG AIMEEQAAEL LGVKMCTFVP RHTRNLANEF RCYVNYDSGL DCGI
Target Information
Methylation of DNA at cytosine residues plays an important role in regulation of gene expression, genomic imprinting and is essential for mammalian development. Hypermethylation of CpG islands in tumor suppressor genes or hypomethylation of bulk genomic DNA may be linked with development of cancer. To date, 3 families of mammalian DNA methyltransferase genes have been identified which include Dnmt1, Dnmt2 and Dnmt3. Dnmt1 is constitutively expressed in proliferating cells and inactivation of this gene causes global demethylation of genomic DNA and embryonic lethality. Dnmt2 is expressed at low levels in adult tissues and its inactivation does not affect DNA methylation or maintenance of methylation. Dnmt2 contains all the sequence motifs diagnostic of DNA (cytosine-5)-methyltransferases but appears to lack the large N-terminal regulatory domain common to other eukaryotic methyltransferases.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: DMNT2; DNMT2; EEF1A lysine methyltransferase 1; eEF1A-KMT; M.HsaIIP; N(6)-adenine-specific DNA methyltransferase 2; N-6 adenine-specific DNA methyltransferase 2 (putative); Protein-lysine N-methyltransferase N6AMT2; PuMet; RNMT1; RP11-406H21.1
Gene Aliases: EEF1AKMT1; ESP13; N6AMT2
UniProt ID: (Human) Q8WVE0
Entrez Gene ID: (Human) 221143
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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