Search Thermo Fisher Scientific
Disclaimer
Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.
Invitrogen
NACA Polyclonal Antibody
Promotion
View available promotion(s)
Promo code: {{promo.promoCode}} {{promo.promoDescription}}. {{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.learnmore']}}
FIGURE: 1 / 1
NACA Antibody (PA5-116506) in WB
Western blot analysis of NACA in extracts of various cell lines. Samples were incubated with NACA Polyclonal antibody (Product # PA5-116506) using a dilution of 1:1,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 45s.
Product Details
PA5-116506
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 1-215 of human NACA (NP_0011066731)
Conjugate
Form
Liquid
Concentration
0.22 mg/mL
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.3, with 50% glycerol
Contains
0.05% ProClin 300
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2901137
Product Specific Information
Positive Samples: HL-60, SW620, 293T, U-87MG, HeLa, Mouse liver, Mouse kidney, Mouse brain, Rat brain
Immunogen sequence: MPGEATETVP ATEQELPQPQ AETGSGTESD SDESVPELEE QDSTQATTQQ AQLAAAAEID EEPVSKAKQS RSEKKARKAM SKLGLRQVTG VTRVTIRKSK NILFVITKPD VYKSPASDTY IVFGEAKIED LSQQAQLAAA EKFKVQGEAV SNIQENTQTP TVQEESEEEE VDETGVEVKD IELVMSQANV SRAKAVRALK NNSNDIVNAI MELTM
Target Information
NACA prevents inappropriate targeting of non-secretory polypeptides to the endoplasmic reticulum (ER). It binds to nascent polypeptide chains as they emerge from the ribosome and blocks their interaction with the signal recognition particle (SRP), which normally targets nascent secretory peptides to the ER. NACA also reduces the inherent affinity of ribosomes for protein translocation sites in the ER membrane (M sites), and may act as a specific coactivator for JUN, binding to DNA and stabilizing the interaction of JUN homodimers with target gene promoters.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
References (0)
Have you cited this product in a publication?
Let us know so we can reference it here.
Bioinformatics
Protein Aliases: Alpha-NAC; Alpha-NAC, muscle-specific form; Alpha-NAC/1.9.2; NAC-alpha; nascent polypeptide-associated complex alpha subunit; Nascent polypeptide-associated complex subunit alpha; Nascent polypeptide-associated complex subunit alpha, muscle-specific form; nascent-polypeptide-associated complex alpha polypeptide; skNAC
Gene Aliases: AL022831; AL024382; Gm1878; HSD48; mKIAA0363; NAC-alpha; NACA; NACA1; skNAC
UniProt ID: (Human) Q13765, (Mouse) P70670
Entrez Gene ID: (Human) 4666, (Rat) 288770, (Mouse) 17938
transcription, DNA-templated
regulation of transcription, DNA-templated
cardiac ventricle development
negative regulation of striated muscle cell apoptotic process
skeletal muscle tissue regeneration
positive regulation of skeletal muscle tissue growth
regulation of skeletal muscle fiber development
myoblast migration
heart trabecula morphogenesis
negative regulation of transcription from RNA polymerase II promoter involved in heart development
positive regulation of transcription from RNA polymerase II promoter involved in heart development
regulation of nucleic acid-templated transcription
positive regulation of cell proliferation involved in heart morphogenesis
transport
protein transport
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
Learn more
We're here to help
Get expert recommendations for common problems or connect directly with an on staff expert for technical assistance related to applications, equipment and general product use.
Contact tech support