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Invitrogen
NAF1 Polyclonal Antibody
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FIGURE: 1 / 1
NAF1 Antibody (PA5-116774) in WB
Western blot analysis of NAF1 in extracts of various cell lines. Samples were incubated with NAF1 Polyclonal antibody (Product # PA5-116774) using a dilution of 1:1,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.
Product Details
PA5-116774
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 150-400 of human NAF1 (NP_6123952)
Conjugate
Form
Liquid
Concentration
0.39 mg/mL
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.3, with 50% glycerol
Contains
0.01% thimerosal
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2901405
Product Specific Information
Positive Samples: K-562, Mouse testis
Immunogen sequence: PPVLSDGDDD LQIEKENKNF PLKTKDELLL NELPSVEELT IILPEDIELK PLGMVSSIIE QLVIIESMTN LPPVNEETVI FKSDRQAAGK IFEIFGPVAH PFYVLRFNSS DHIESKGIKI KETMYFAPSM KDFTQYIFTE KLKQDKGSDA SWKNDQEPPP EALDFSDDEK EKEAKQRKKS QIQGRKKLKS EFNEPGEDFT EVHQNWNAHS SASEHAKGYR NREFTRGFSR ARYPRSCHGR PPPQHFYNSE H
Target Information
RNA-binding protein required for the maturation of box H/ACA snoRNPs complex and ribosome biogenesis. During assembly of the H/ACA snoRNPs complex, it associates with the complex and disappears during maturation of the complex and is replaced by NOLA1/GAR1 to yield mature H/ACA snoRNPs complex. Probably competes with NOLA1/GAR1 for binding with DKC1/NOLA4.
⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: H/ACA ribonucleoprotein complex non-core subunit NAF1; hNAF1; nuclear assembly factor 1 homolog
Gene Aliases: Gm174; NAF1; RGD1306802
UniProt ID: (Human) Q96HR8, (Rat) Q52KK4, (Mouse) Q3UMQ8
Entrez Gene ID: (Human) 92345, (Rat) 306387, (Mouse) 234344
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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