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Invitrogen
NAIF1 Polyclonal Antibody
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FIGURE: 1 / 1
NAIF1 Antibody (PA5-90302) in WB
Western blot analysis of NAIF1 in extracts of various cell lines. Samples were incubated with NAIF1 Polyclonal antibody (Product # PA5-90302) using a dilution of 1:1,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.
Product Details
PA5-90302
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 1-110 of human NAIF1 (NP_9310451)
Conjugate
Form
Liquid
Concentration
1.18 mg/mL
Purification
Affinity Chromatography
Storage buffer
PBS, pH 7.3, with 50% glycerol
Contains
0.01% thimerosal
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Wet ice
RRID
AB_2806011
Product Specific Information
Immunogen sequence: MAVPAKKRKM NFSEREVEII VEELELKKHL LVNHFNAGVP LAAKSAAWHG ILRRVNAVAT CRRELPEVKK KWSDLKTEVR RKVAQVRAAV EGGEAPGPTE EDGAGGPGTG; Positive Samples: LO2, A375, 293T, OVCAR3, Mouse brain, Mouse liver, Rat brain, Rat liver; Cellular Location: Nucleus
Target Information
Human NAIF1 is located on chromosome 9q34.11 and encodes 327 amino acids with a homeodomain-like region and two nuclear localization signals at its N-terminal region. NAIF1 is conserved across diverse species, including human, mouse, crab-eating macaque, dog, chicken and frog, and shares no obvious homology to any known genes or proteins. NAIF1 was predominantly localized in the nucleus. Overexpression of NAIF1 inhibited cell growth and induced apoptosis. Furthermore, NAIF1 transfection caused both decreases in mitochondrial membrane potential and caspase-3 activation. In summary, NAIF1 is a nuclear protein that induces apoptosis when overexpressed.
⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: nuclear apoptosis inducing factor 1; Nuclear apoptosis-inducing factor 1
Gene Aliases: 2310007O20Rik; 4933440H19Rik; bA379C10.2; C9orf90; NAIF1
UniProt ID: (Human) Q69YI7, (Mouse) Q6PFD7
Entrez Gene ID: (Human) 203245, (Mouse) 71254, (Rat) 687739
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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