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NARFL Antibody (A305-769A-T) in IP
Detection of Human NARFL by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-NARFL antibody A305-769A-M (lot A305-769A-M-1) used for IP at 8 µl per reaction. NARFL was also immunoprecipitated by rabbit anti-NARFL antibody A305-770A-M. For blotting immunoprecipitated NARFL, A305-769A-M was used at 1:400. Detection: Chemiluminescence with an exposure time of 30 second... View More
Product Details
A305-769A-T
Product Specifications
Species Reactivity
Human
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
A region between residue 50 and 100 of human Cytosolic Fe-S cluster assembly factor NARFL.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
phosphate/tris citrate, pH 7.0 to 8.0
Contains
0.09% sodium azide
Storage conditions
4°C
Shipping conditions
Wet ice
Product Specific Information
Predicted reactivity by homology: Orangutan.
Target Information
Component of the cytosolic iron-sulfur protein assembly (CIA) complex, a multiprotein complex that mediates the incorporation of iron-sulfur cluster into extramitochondrial Fe/S proteins. Seems to negatively regulate the level of HIF1A expression, although this effect could be indirect.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Cytosolic Fe-S cluster assembly factor NARFL; Cytosolic iron-sulfur assembly component 3; IOP1; Iron-only hydrogenase-like protein 1; LET1 like/JFP15; nuclear prelamin A recognition factor-like; Nuclear prelamin A recognition factor-like protein; Protein related to Narf
Gene Aliases: CIAO3; HPRN; IOP1; LET1L; NAR1; NARFL; PRN
UniProt ID: (Human) Q9H6Q4
Entrez Gene ID: (Human) 64428
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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