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Invitrogen
NASP Polyclonal Antibody
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FIGURE: 1 / 7
NASP Antibody (PA5-106317) in ICC/IF
Immunofluorescent analysis of NASP in HeLa cells. Samples were fixed with paraformaldehyde, permeabilized with 0.1% Triton X-100, blocked with 10% serum (45 min at 25°C), incubated with mouse anti-beta tubulin and NASP polyclonal antibody (Product # PA5-106317) using a dilution of 1:200 (1 hr, 37°C), and followed by goat anti-rabbit IgG Alexa Fluor 594 (red) and goat anti-mouse IgG Alexa Fluor 488 (green).
Product Details
PA5-106317
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
A synthesized peptide derived from human NASP(Accession P49321), corresponding to amino acid residues S677-P727.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.4, with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20°C
Shipping conditions
Wet ice
RRID
AB_2853994
Product Specific Information
Antibody detects endogenous levels of total NASP.
Target Information
NASP encodes a H1 histone binding protein that is involved in transporting histones into the nucleus of dividing cells. Multiple isoforms are encoded by transcript variants of this gene. The somatic form is expressed in all mitotic cells, is localized to the nucleus, and is coupled to the cell cycle. The testicular form is expressed in embryonic tissues, tumor cells, and the testis. In male germ cells, this protein is localized to the cytoplasm of primary spermatocytes, the nucleus of spermatids, and the periacrosomal region of mature spermatozoa.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: DKFZp547F162; FLJ31599; FLJ35510; histone H1-binding protein; MGC19722; MGC20372; MGC2297; NASP; NASP histone chaperone; nasp {ECO:0000312|EMBL:AAH81913.1}; Nuclear autoantigenic sperm protein; nuclear autoantigenic sperm protein (histone-binding); somatic histone binding protein NASP
Gene Aliases: 5033430J04Rik; AI131596; AI317140; D4Ertd767e; Epcs32; FLB7527; HMDRA1; NASP; Nasp-T; PRO1999
UniProt ID: (Human) P49321, (Rat) Q66HD3, (Mouse) Q99MD9
Entrez Gene ID: (Human) 4678, (Rat) 298441, (Mouse) 50927
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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