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FIGURE: 1 / 3
NBPF6 Antibody (PA5-103962) in ICC/IF
Immunocytochemistry analysis of NBPF6 in A549 cells. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25°C. Samples were incubated with NBPF6 polyclonal antibody (Product # PA5-103962) and mouse anti-beta tubulin antibody for 1 hour at 37°C. An AlexaFluor594 conjugated goat anti-rabbit IgG (Red) and an AlexaFluor488 conjugated goat anti-mouse IgG (Green) were used as the secondary antibodies. The nuclear counter stain is DAPI (blue).
Product Details
PA5-103962
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
A synthesized peptide derived from human NBPF6(Accession Q5VWK0), corresponding to amino acid residues E505-L555.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.4, with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20°C
Shipping conditions
Wet ice
RRID
AB_2853294
Product Specific Information
Antibody detects endogenous levels of total NBPF6.
Target Information
Members of the neuroblastoma breakpoint family are encoded by genes that map to a region of segmental duplications on human chromosome 1. Both NBPF4 (neuroblastoma breakpoint family member 4) and NBPF6 (neuroblastoma breakpoint family member 6) are cytoplasmic proteins that belong to the NBPF family. NBPF4 contains 123 amino acids and is expressed in testis, while NBPF6 consists of 638 amino acids and contains 3 NBPF domains. Like other NBPF family members, both proteins are encoded by genes that map to human chromosome 1p13.3. Human chromosome 1 spans 260 million base pairs, contains over 3,000 genes, comprises nearly 8% of the human genome and houses a large number of disease-associated genes, including those that are involved in familial adenomatous polyposis, Stickler syndrome, Parkinson's disease, Gaucher disease, schizophrenia and Usher syndrome.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: NBPF family member NBPF6; Neuroblastoma breakpoint family member 6; neuroblastoma breakpoint family, member 6
Gene Aliases: NBPF6
UniProt ID: (Human) Q5VWK0
Entrez Gene ID: (Human) 653149
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