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NBS1 Antibody (711701) in ICC/IF
For immunofluorescence analysis, A549 cells were fixed and permeabilized for detection of endogenous NBS1 using Anti- NBS1 Recombinant Rabbit Superclonal™ Antibody (Product # 711701, 2µg/mL) and labeled with Goat anti-Rabbit IgG (Heavy Chain) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A27034, 1:2000). Panel a) shows representative cells that were stained for detection and localization of NBS1 (Nibrin)/NBN protein (green), Panel b) is stained for nuclei (blue) using SlowFade® Gold Antifade Mountant with DAPI (Pro... View More
Product Details
711701
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse
Host/Isotype
Rabbit
/ IgG
Expression System
Expi293
Class
Recombinant Superclonal
Type
Antibody
Immunogen
Protein corresponding to human NBS1 (aa400-aa754)
Conjugate
Form
Liquid
Concentration
0.5 mg/mL
Purification
Protein A
Storage buffer
PBS, pH 7.4
Contains
0.09% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Wet ice
RRID
AB_2688316
Product Specific Information
This antibody is predicted to react with Monkey, Bovine, Horse, and Sheep.
Recombinant rabbit Superclonal™ antibodies are unique offerings from Thermo Fisher Scientific. They are comprised of a selection of multiple different recombinant monoclonal antibodies, providing the best of both worlds - the sensitivity of polyclonal antibodies with the specificity of monoclonal antibodies - all delivered with the consistency only found in a recombinant antibody. While functionally the same as a polyclonal antibody - recognizing multiple epitope sites on the target and producing higher detection sensitivity for low abundance targets - a recombinant rabbit Superclonal™ antibody has a known mixture of light and heavy chains. The exact population can be produced in every lot, circumventing the biological variability typically associated with polyclonal antibody production. Note: Formerly called “Recombinant polyclonal antibody”, this product is now rebranded as “Recombinant Superclonal™ antibody”. The physical product and the performance remain unchanged.
Target Information
The p95 gene (identical to NBS1 and nibrin) is a member of the hMre11/hRad50 double-strand break complex. This protein complex has been implicated in Nijmegen breakage syndrome, an autosomal recessive disorder marked by increased cancer incidence, cell cycle checkpoint deficits, and ionizing radiation sensitivity, thus revealing a direct molecular link between double-strand break repair and cell cycle checkpoint functions.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: AT V1; AT V2; Cell cycle regulatory protein p95; FLJ10155; hNbs1; MGC87362; Nibrin; Nijmegen breakage syndrome 1 (nibrin); Nijmegen breakage syndrome protein 1; Nijmegen breakage syndrome protein 1 homolog; OTTHUMP00000204088; p95 protein of the MRE11/RAD50 complex; p95-NBS1
Gene Aliases: AT-V1; AT-V2; ATV; NBN; NBS; NBS1; P95
UniProt ID: (Human) O60934, (Mouse) Q9R207
Entrez Gene ID: (Human) 4683, (Mouse) 27354
DNA damage checkpoint
telomere maintenance
double-strand break repair via homologous recombination
DNA double-strand break processing
DNA synthesis involved in DNA repair
strand displacement
blastocyst growth
DNA replication
double-strand break repair
double-strand break repair via nonhomologous end joining
cell cycle arrest
mitotic cell cycle checkpoint
mitotic G2 DNA damage checkpoint
cell proliferation
regulation of DNA-dependent DNA replication initiation
DNA damage response, signal transduction by p53 class mediator
telomeric 3' overhang formation
positive regulation of protein autophosphorylation
positive regulation of telomere maintenance
DNA duplex unwinding
positive regulation of kinase activity
isotype switching
neuromuscular process controlling balance
meiotic cell cycle
intrinsic apoptotic signaling pathway
regulation of signal transduction by p53 class mediator
negative regulation of telomere capping
in utero embryonic development
DNA repair
cellular response to DNA damage stimulus
cell cycle
positive regulation of cell proliferation
negative regulation of neuron differentiation
negative regulation of viral entry into host cell
regulation of fibroblast proliferation
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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