Proteintech

NCALD Polyclonal Antibody

NCALD Antibody in Immunohistochemistry (Paraffin) (IHC (P))

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NCALD Antibody (12925-1-AP) in IHC (P)

Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using (Product # 12925-1-AP) (NCALD antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Product Details

12925-1-AP

Applications

Tested Dilution

Publications

Western Blot (WB)

1:1,000-1:4,000

View 7 publications 7 publications

Immunohistochemistry (IHC)

View 5 publications 5 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:50-1:500

Immunocytochemistry (ICC/IF)

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Human, Mouse

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

NCALD Fusion Protein Ag3614 (1-193 aa encoded by BC036098)

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.51 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C

Shipping conditions

Wet ice (domestic); Dry ice (international)

Product Specific Information

Immunogen sequence: MGKQNSKLR PEVMQDLLES TDFTEHEIQE WYKGFLRDCP SGHLSMEEFK KIYGNFFPYG DASKFAEHVF RTFDANGDGT IDFREFIIAL SVTSRGKLEQ KLKWAFSMYD LDGNGYISKA EMLEIVQAIY KMVSSVMKMP EDESTPEKRT EKIFRQMDTN RDGKLSMEEF IRGAKSDPSI VRLLQCDPSS AGQF (1-193 aa encoded by BC036098)

Target Information

This gene encodes a member of the neuronal calcium sensor (NCS) family of calcium-binding proteins. The protein contains an N-terminal myristoylation signal and four EF-hand calcium binding loops. The protein is cytosolic at resting calcium levels; however, elevated intracellular calcium levels induce a conformational change that exposes the myristoyl group, resulting in protein association with membranes and partial co-localization with the perinuclear trans-golgi network. The protein is thought to be a regulator of G protein-coupled receptor signal transduction. Several alternatively spliced variants of this gene have been determined, all of which encode the same protein; additional variants may exist but their biological validity has not been determined.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Long-Term SMN- and Ncald-ASO Combinatorial Therapy in SMA Mice and NCALD-ASO Treatment in hiPSC-Derived Motor Neurons Show Protective Effects.

International journal of molecular sciences

Muiños-Bühl A,Rombo R,Ling KK,Zilio E,Rigo F,Bennett CF,Wirth B

Mon Feb 20 00:00:00 UTC 2023

N6‑methyladenosine upregulates miR‑181d‑5p in exosomes derived from cancer‑associated fibroblasts to inhibit 5‑FU sensitivity by targeting NCALD in colorectal cancer.

International journal of oncology

Pan S,Deng Y,Fu J,Zhang Y,Zhang Z,Qin X

Tue Feb 01 00:00:00 UTC 2022

N6‑methyladenosine upregulates miR‑181d‑5p in exosomes derived from cancer‑associated fibroblasts to inhibit 5‑FU sensitivity by targeting NCALD in colorectal cancer.

International journal of oncology

Pan S,Deng Y,Fu J,Zhang Y,Zhang Z,Qin X

Tue Feb 01 00:00:00 UTC 2022

NCALD as a potential predictive biomarker for the efficacy of platinum-based chemotherapy in ovarian cancer.

Biomarkers in medicine

Konrad SM,Schwamborn K,Krüger A,Honert K,Schmitt M,Hellmann D,Schmalfeldt B,Meindl A,Kiechle M,Quante AS,Brambs C,Grill S,Ramser J

Sat Oct 01 00:00:00 UTC 2022

NCALD as a potential predictive biomarker for the efficacy of platinum-based chemotherapy in ovarian cancer.

Biomarkers in medicine

Konrad SM,Schwamborn K,Krüger A,Honert K,Schmitt M,Hellmann D,Schmalfeldt B,Meindl A,Kiechle M,Quante AS,Brambs C,Grill S,Ramser J

Sat Oct 01 00:00:00 UTC 2022

Transcriptome and Proteome Analysis in LUHMES Cells Overexpressing Alpha-Synuclein.

Frontiers in neurology

Höllerhage M,Stepath M,Kohl M,Pfeiffer K,Chua OWH,Duan L,Hopfner F,Eisenacher M,Marcus K,Höglinger GU

Sat Apr 30 00:00:00 UTC 2022

Neurocalcin Delta Knockout Impairs Adult Neurogenesis Whereas Half Reduction Is Not Pathological.

Frontiers in molecular neuroscience

Upadhyay A,Hosseinibarkooie S,Schneider S,Kaczmarek A,Torres-Benito L,Mendoza-Ferreira N,Overhoff M,Rombo R,Grysko V,Kye MJ,Kononenko NL,Wirth B

Thu Oct 01 00:00:00 UTC 2020

Neurocalcin Delta Knockout Impairs Adult Neurogenesis Whereas Half Reduction Is Not Pathological.

Frontiers in molecular neuroscience

Upadhyay A,Hosseinibarkooie S,Schneider S,Kaczmarek A,Torres-Benito L,Mendoza-Ferreira N,Overhoff M,Rombo R,Grysko V,Kye MJ,Kononenko NL,Wirth B

Thu Oct 01 00:00:00 UTC 2020

Clinicopathological and prognostic significance of NCALD protein expression in lung adenocarcinoma.

Translational cancer research

Chen Y,Feng X,Shen H,Zhang L,Li X,Gao L,Zhang X,Wang B

Sat Jun 01 00:00:00 UTC 2019

Neurocalcin Delta Suppression Protects against Spinal Muscular Atrophy in Humans and across Species by Restoring Impaired Endocytosis.

American journal of human genetics

Riessland M,Kaczmarek A,Schneider S,Swoboda KJ,Löhr H,Bradler C,Grysko V,Dimitriadi M,Hosseinibarkooie S,Torres-Benito L,Peters M,Upadhyay A,Biglari N,Kröber S,Hölker I,Garbes L,Gilissen C,Hoischen A,Nürnberg G,Nürnberg P,Walter M,Rigo F,Bennett CF,Kye MJ,Hart AC,Hammerschmidt M,Kloppenburg P,Wirth B

Thu Feb 02 00:00:00 UTC 2017

Neurocalcin Delta Suppression Protects against Spinal Muscular Atrophy in Humans and across Species by Restoring Impaired Endocytosis.

American journal of human genetics

Thu Feb 02 00:00:00 UTC 2017

Upregulation of long intergenic noncoding RNA 00673 promotes tumor proliferation via LSD1 interaction and repression of NCALD in non-small-cell lung cancer.

Oncotarget

Shi X,Ma C,Zhu Q,Yuan D,Sun M,Gu X,Wu G,Lv T,Song Y

Tue May 03 00:00:00 UTC 2016

Upregulation of long intergenic noncoding RNA 00673 promotes tumor proliferation via LSD1 interaction and repression of NCALD in non-small-cell lung cancer.

Oncotarget

Shi X,Ma C,Zhu Q,Yuan D,Sun M,Gu X,Wu G,Lv T,Song Y

Tue May 03 00:00:00 UTC 2016

Upregulation of long intergenic noncoding RNA 00673 promotes tumor proliferation via LSD1 interaction and repression of NCALD in non-small-cell lung cancer.

Oncotarget

Shi X,Ma C,Zhu Q,Yuan D,Sun M,Gu X,Wu G,Lv T,Song Y

Tue May 03 00:00:00 UTC 2016

Upregulation of long intergenic noncoding RNA 00673 promotes tumor proliferation via LSD1 interaction and repression of NCALD in non-small-cell lung cancer.

Oncotarget

Shi X,Ma C,Zhu Q,Yuan D,Sun M,Gu X,Wu G,Lv T,Song Y

Tue May 03 00:00:00 UTC 2016

Gsx2 controls region-specific activation of neural stem cells and injury-induced neurogenesis in the adult subventricular zone.

Genes & development

López-Juárez A,Howard J,Ullom K,Howard L,Grande A,Pardo A,Waclaw R,Sun YY,Yang D,Kuan CY,Campbell K,Nakafuku M

Sat Jun 01 00:00:00 UTC 2013

Gsx2 controls region-specific activation of neural stem cells and injury-induced neurogenesis in the adult subventricular zone.

Genes & development

López-Juárez A,Howard J,Ullom K,Howard L,Grande A,Pardo A,Waclaw R,Sun YY,Yang D,Kuan CY,Campbell K,Nakafuku M

Sat Jun 01 00:00:00 UTC 2013

Bioinformatics

Protein Aliases: Neurocalcin-delta

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Gene Aliases: AI848120; D030020D09Rik; D15Ertd412e; NCALD

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UniProt ID: (Human) P61601, (Rat) Q5PQN0, (Mouse) Q91X97

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Entrez Gene ID: (Human) 83988, (Rat) 553106, (Mouse) 52589

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Function(s)

actin binding calcium ion binding protein binding ligand-gated ion channel activity tubulin binding clathrin binding alpha-tubulin binding metal ion binding

Process(es)

regulation of systemic arterial blood pressure calcium-mediated signaling vesicle-mediated transport ion transmembrane transport

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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NCALD Polyclonal Antibody

NCALD Antibody (12925-1-AP) in IHC (P)

Product Details

12925-1-AP

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (Paraffin) (IHC (P))

Immunocytochemistry (ICC/IF)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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