Invitrogen

NCBP1 Polyclonal Antibody

NCBP1 Antibody in Immunocytochemistry (ICC/IF)

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NCBP1 Antibody (PA5-30098) in ICC/IF

Immunofluorescence analysis of NCBP1 was performed using 70% confluent log phase HeLa cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with NCBP1 Polyclonal Antibody (Product # PA5-30098) at 1:100 dilution in 0.1% BSA, incubated at 4 degree Celsius overnight and then labeled with Goat anti-Rabbit IgG (Heavy Chain) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A2703... View More

NCBP1 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

Product Image Gallery

NCBP1 Antibody — Cell growth and poly(A) RNA distribution after knockdown of NCBP1 or NCBP2. (a) Growth of HeLa cells after RNAi-mediated knockdown (left panel). Cells were treated twice with siRNAs against NCBP1, NCBP2, NXF1 or non-targeting siRNA control (siScrambled), and cell titre determined by a luminescence-based cell viability assay at the indicated time points. The graph shows the means.d. of two individual treatments measured in triplicates. One representative experiment of three is shown. RLU, relative light units. Knockdown efficiency of NCBP1 and NCBP2 was confirmed by western blotting against indicated proteins (right panel). Asterisk: nonspecific band. (b) Poly(A) RNA distribution in HeLa cells 48 h after repeated RNAi-mediated knockdown. HeLa cells were transfected with the indicated siRNAs, and localization of poly(A) RNA (red) was stained by RNA fluorescence in situ hybridization (RNA-FISH) using fluorescently labelled oligo (dT) as probe. DAPI (blue) was used to visualize nuclei. Shown confocal images are representative for three independent experiments. Scale bar, 20 µm. (c) Abundance of NCBP1 and NCBP2 in HeLa cells. Complete HeLa cell lysates were analysed by LC-MS/MS. Shown are average iBAQ intensities for NCBP1 and NCBP2 of 11 measurementss.d. {KD}

Product Details

PA5-30098

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:3,000

Immunohistochemistry (Paraffin) (IHC (P))

1:100-1:1,000

Immunocytochemistry (ICC/IF)

1:100-1:1,000

Miscellaneous PubMed (MISC)

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human, Mouse

Published species

Human

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Recombinant protein encompassing a sequence within the N-terminus region of human CBP80. The exact sequence is proprietary.

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

0.1M tris glycine, pH 7, with 20% glycerol

Contains

0.01% thimerosal

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Ambient (domestic); Wet ice (international)

AB_2547572

Product Specific Information

Recommended positive controls: A549, HeLa, HepG2.

Predicted reactivity: Mouse (98%), Rat (98%), Xenopus laevis (81%), Chicken (91%).

Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

Target Information

May be involved in protein trafficking from the plasma membrane to the early endosome (EE) as well as in homotypic fusion of endocytic organelles. Mediates the endocytic trafficking from early endosomes to late endosomes and lysosomes.

⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

mRNA export through an additional cap-binding complex consisting of NCBP1 and NCBP3.

Nature communications

Gebhardt A,Habjan M,Benda C,Meiler A,Haas DA,Hein MY,Mann A,Mann M,Habermann B,Pichlmair A

PA5-30098 was used in immunocytochemistry and western blot to elucidate the roles of nuclear cap-binding protein 1-3 in mRNA biogenesis

Fri Sep 18 00:00:00 UTC 2015

Bioinformatics

Protein Aliases: 80 kDa nuclear cap-binding protein; CBP80; MGC2087; NCBP 80 kDa subunit; nuclear cap binding protein subunit 1, 80kD; nuclear cap binding protein subunit 1, 80kDa; Nuclear cap-binding protein subunit 1; RP11-546O6.1

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Gene Aliases: AU014645; AW538051; CBP80; NCBP; NCBP1; Sto1

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UniProt ID: (Human) Q09161, (Mouse) Q3UYV9

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Entrez Gene ID: (Human) 4686, (Mouse) 433702

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Function(s)

RNA cap binding RNA binding protein binding poly(A) RNA binding

Process(es)

nuclear-transcribed mRNA catabolic process, nonsense-mediated decay mRNA splicing, via spliceosome transcription from RNA polymerase II promoter transcription elongation from RNA polymerase II promoter termination of RNA polymerase II transcription 7-methylguanosine mRNA capping RNA export from nucleus mRNA export from nucleus regulation of translational initiation histone mRNA metabolic process RNA splicing fibroblast growth factor receptor signaling pathway gene expression positive regulation of cell growth gene silencing by RNA mRNA 3'-end processing positive regulation of mRNA 3'-end processing snRNA transcription from RNA polymerase II promoter mRNA cis splicing, via spliceosome nuclear export pre-mRNA cleavage required for polyadenylation regulation of mRNA polyadenylation mRNA cleavage mRNA processing regulation of translation transport RNA metabolic process mRNA transport

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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NCBP1 Polyclonal Antibody

NCBP1 Antibody (PA5-30098) in ICC/IF

Product Details

PA5-30098

Western Blot (WB)

Immunohistochemistry (Paraffin) (IHC (P))

Immunocytochemistry (ICC/IF)

Miscellaneous PubMed (MISC)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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