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Invitrogen
NCK1 Monoclonal Antibody (5B7)
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Product Details
MA5-17132
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Non-human primate
Host/Isotype
Mouse
/ IgG1
Class
Monoclonal
Type
Antibody
Clone
5B7
Immunogen
Purified recombinant fragment of human NCK1 expressed in E. Coli.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Protein G
Storage buffer
PBS
Contains
0.05% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2538603
Product Specific Information
MA5-17132 targets NCK1 in FACS, IHC, indirect ELISA, and WB applications and shows reactivity with Human and Non-human primate samples.
The MA5-17132 immunogen is purified recombinant fragment of human NCK1 expressed in E. Coli.
MA5-17132 detects NCK1 which has a predicted molecular weight of approximately 47kDa.
Target Information
NCK1 is one of the signaling and transforming proteins containing Src homology 2 and 3 (SH2 and SH3) domains. It is located in the cytoplasm and is an adaptor protein involved in transducing signals from receptor tyrosine kinases to downstream signal recipients such as RAS. Alternatively spliced transcript variants encoding different isoforms have been found.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Cytoplasmic protein NCK1; melanoma NCK protein; MGC12668; NCK adapter protein 1; NCK tyrosine kinase; Nck-1; non-catalytic region of tyrosine kinase; SH2/SH3 adapter protein NCK-alpha; SH2/SH3 adapter protein NCK1; SH2/SH3 adaptor protein NCK-alpha
Gene Aliases: NCK; nck-1; NCK1; NCKalpha
UniProt ID: (Human) P16333
Entrez Gene ID: (Human) 4690
protein kinase inhibitor activity
SH3/SH2 adaptor activity
ARF guanyl-nucleotide exchange factor activity
receptor binding
protein binding
cytoskeletal adaptor activity
protein domain specific binding
receptor signaling complex scaffold activity
protein binding, bridging
receptor tyrosine kinase binding
eukaryotic initiation factor eIF2 binding
cadherin binding involved in cell-cell adhesion
negative regulation of protein kinase activity
substrate-dependent cell migration, cell extension
actin filament organization
signal complex assembly
vesicle-mediated transport
lamellipodium assembly
positive regulation of actin filament polymerization
negative regulation of peptidyl-serine phosphorylation
positive regulation of translation in response to endoplasmic reticulum stress
Fc-gamma receptor signaling pathway involved in phagocytosis
positive regulation of T cell proliferation
T cell activation
positive regulation of GTPase activity
positive regulation of transcription from RNA polymerase II promoter
vascular endothelial growth factor receptor signaling pathway
T cell receptor signaling pathway
response to other organism
negative regulation of cell death
peptidyl-serine dephosphorylation
positive regulation of endoplasmic reticulum stress-induced intrinsic apoptotic signaling pathway
positive regulation of cap-dependent translational initiation
positive regulation of cap-independent translational initiation
negative regulation of PERK-mediated unfolded protein response
negative regulation of endoplasmic reticulum stress-induced eIF2 alpha phosphorylation
negative regulation of transcription from RNA polymerase II promoter in response to endoplasmic reticulum stress
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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