Invitrogen

NCK1 Monoclonal Antibody (K.349.1)

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Datasheet
Protocols
Questions & Answers
Datasheet
Protocols
Questions & Answers

Product Details

MA5-15024

Applications
Tested Dilution
Publications

Western Blot (WB)

1:1,000-1:5,000
-

Immunoprecipitation (IP)

1:50
-
Product Specifications

Species Reactivity

Human, Mouse, Non-human primate, Rat

Host/Isotype

Rabbit / IgG

Class

Monoclonal

Type

Antibody

Clone

K.349.1

Immunogen

Synthetic peptide corresponding to residues surrounding Gly300 of human NCK1
View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

282 µg/mL

Purification

Affinity chromatography

Storage buffer

0.01M HEPES, pH 7.5, with 0.15M NaCl, 100µg/mL BSA, 50% glycerol

Contains

<0.02% sodium azide

Storage conditions

-20°C

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_10982803

Product Specific Information

It is not recommended to aliquot this antibody.

Target Information

NCK1 is one of the signaling and transforming proteins containing Src homology 2 and 3 (SH2 and SH3) domains. It is located in the cytoplasm and is an adaptor protein involved in transducing signals from receptor tyrosine kinases to downstream signal recipients such as RAS. Alternatively spliced transcript variants encoding different isoforms have been found.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: Cytoplasmic protein NCK1; melanoma NCK protein; MGC12668; NCK adapter protein 1; NCK adaptor protein 1; NCK tyrosine kinase; Nck-1; non-catalytic region of tyrosine kinase; non-catalytic region of tyrosine kinase adaptor protein 1; SH2/SH3 adapter protein NCK-alpha; SH2/SH3 adapter protein NCK1; SH2/SH3 adaptor protein NCK-alpha

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Gene Aliases: 6330586M15Rik; D230010O13Rik; NCK; Nck-1; NCK1; NCKalpha

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UniProt ID: (Human) P16333, (Mouse) Q99M51

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Entrez Gene ID: (Human) 4690, (Rat) 300955, (Mouse) 17973

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Function(s)
protein kinase inhibitor activity SH3/SH2 adaptor activity ARF guanyl-nucleotide exchange factor activity receptor binding protein binding cytoskeletal adaptor activity protein domain specific binding receptor signaling complex scaffold activity protein binding, bridging receptor tyrosine kinase binding eukaryotic initiation factor eIF2 binding cadherin binding involved in cell-cell adhesion protein kinase regulator activity
Process(es)
negative regulation of protein kinase activity substrate-dependent cell migration, cell extension actin filament organization cell migration lamellipodium assembly positive regulation of actin filament polymerization positive regulation of translation in response to stress negative regulation of peptidyl-serine phosphorylation positive regulation of T cell proliferation T cell activation positive regulation of transcription from RNA polymerase II promoter response to other organism negative regulation of cell death regulation of endoplasmic reticulum stress-induced eIF2 alpha phosphorylation peptidyl-serine dephosphorylation negative regulation of endoplasmic reticulum unfolded protein response positive regulation of endoplasmic reticulum stress-induced intrinsic apoptotic signaling pathway positive regulation of cap-dependent translational initiation positive regulation of cap-independent translational initiation negative regulation of transcription from RNA polymerase II promoter in response to endoplasmic reticulum stress signal complex assembly vesicle-mediated transport positive regulation of translation in response to endoplasmic reticulum stress Fc-gamma receptor signaling pathway involved in phagocytosis positive regulation of GTPase activity vascular endothelial growth factor receptor signaling pathway T cell receptor signaling pathway negative regulation of PERK-mediated unfolded protein response negative regulation of endoplasmic reticulum stress-induced eIF2 alpha phosphorylation regulation of translation
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