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Invitrogen
NCK2 Polyclonal Antibody
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NCK2 Antibody (PA5-104540) in IHC (P)
Immunohistochemistry analysis of paraffin-embedded NCK2 in human lymph node tissue sections. Antigen retrieval was performed using citrate buffer. Samples were blocked with blocking buffer (1.5 hr, 22°C), incubated with NCK2 polyclonal antibody (Product # PA5-104540) using a dilution of 1:100 (1.5 hr, 22°C), followed by HRP conjugated goat anti-rabbit.
Product Details
PA5-104540
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
A synthesized peptide derived from human NCK2(Accession O43639), corresponding to amino acid residues Y164-L214.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.4, with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20°C
Shipping conditions
Wet ice
RRID
AB_2853840
Product Specific Information
Antibody detects endogenous levels of total NCK2.
Target Information
The NCK family of SH2/SH3 adaptor proteins consists of two members, NCK1 and NCK2, which couple tyrosine kinase signaling, including the EGF and PDGF receptor-pathways, to downstream signaling proteins. The NCK2 protein binds human PDGFR-b (Tyr-1009); overexpression of NCK2 inhibits PDGF-induced membrane ruffling and lamellipod formation.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Cytoplasmic protein NCK2; Growth factor receptor-bound protein 4; NCK adaptor protein 2; Nck-2; noncatalytic region of tyrosine kinase, beta; SH2/SH3 adaptor protein NCK-beta
Gene Aliases: 4833426I10Rik; GRB4; Nck-2; NCK2; NCKbeta
UniProt ID: (Human) O43639, (Mouse) O55033
Entrez Gene ID: (Human) 8440, (Mouse) 17974
immunological synapse formation
regulation of translation
actin filament organization
cell migration
lamellipodium assembly
positive regulation of actin filament polymerization
negative regulation of peptidyl-serine phosphorylation
positive regulation of translation in response to endoplasmic reticulum stress
positive regulation of T cell proliferation
positive regulation of transcription from RNA polymerase II promoter
ephrin receptor signaling pathway
dendritic spine development
positive regulation of endoplasmic reticulum stress-induced intrinsic apoptotic signaling pathway
negative regulation of PERK-mediated unfolded protein response
negative regulation of endoplasmic reticulum stress-induced eIF2 alpha phosphorylation
negative regulation of transcription from RNA polymerase II promoter in response to endoplasmic reticulum stress
signal transduction
signal complex assembly
epidermal growth factor receptor signaling pathway
regulation of epidermal growth factor-activated receptor activity
negative regulation of cell proliferation
vesicle-mediated transport
T cell activation
positive regulation of GTPase activity
vascular endothelial growth factor receptor signaling pathway
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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