Invitrogen

NCK2 Polyclonal Antibody

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Datasheet
Protocols
Questions & Answers

Product Details

PA5-96788

Applications
Tested Dilution
Publications

Western Blot (WB)

1:500-1:2,000
-

Immunohistochemistry (Paraffin) (IHC (P))

1:50-1:200
-

ELISA (ELISA)

1 µg/mL
-
Product Specifications

Species Reactivity

Human, Mouse, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 1-380 of human NCK2 (NP_0035722)
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Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

4.95 mg/mL

Purification

Affinity Chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C, Avoid Freeze/Thaw Cycles

Shipping conditions

Wet ice

RRID

AB_2808590

Product Specific Information

Immunogen sequence: MTEEVIVIAK WDYTAQQDQE LDIKKNERLW LLDDSKTWWR VRNAANRTGY VPSNYVERKN SLKKGSLVKN LKDTLGLGKT RRKTSARDAS PTPSTDAEYP ANGSGADRIY DLNIPAFVKF AYVAEREDEL SLVKGSRVTV MEKCSDGWWR GSYNGQIGWF PSNYVLEEVD EAAAESPSFL SLRKGASLSN GQGSRVLHVV QTLYPFSSVT EEELNFEKGE TMEVIEKPEN DPEWWKCKNA RGQVGLVPKN YVVVLSDGPA LHPAHAPQIS YTGPSSSGRF AGREWYYGNV TRHQAECALN ERGVEGDFLI RDSESSPSDF SVSLKASGKN KHFKVQLVDN VYCIGQRRFH TMDELVEHYK KAPIFTSEHG EKLYLVRALQ; Positive Samples: 293T, Mouse lung, Rat brain; Cellular Location: Cytoplasm, Endoplasmic reticulum

Target Information

The NCK family of SH2/SH3 adaptor proteins consists of two members, NCK1 and NCK2, which couple tyrosine kinase signaling, including the EGF and PDGF receptor-pathways, to downstream signaling proteins. The NCK2 protein binds human PDGFR-b (Tyr-1009); overexpression of NCK2 inhibits PDGF-induced membrane ruffling and lamellipod formation.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: Cytoplasmic protein NCK2; Growth factor receptor-bound protein 4; NCK adaptor protein 2; Nck-2; non-catalytic region of tyrosine kinase adaptor protein 2; noncatalytic region of tyrosine kinase, beta; SH2/SH3 adaptor protein NCK-beta

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Gene Aliases: 4833426I10Rik; GRB4; Nck-2; NCK2; NCKbeta

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UniProt ID: (Human) O43639, (Mouse) O55033

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Entrez Gene ID: (Human) 8440, (Mouse) 17974, (Rat) 316369

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Function(s)
SH3/SH2 adaptor activity ARF guanyl-nucleotide exchange factor activity protein binding cytoskeletal adaptor activity receptor signaling complex scaffold activity protein complex binding scaffold protein binding kinase activity
Process(es)
immunological synapse formation regulation of translation actin filament organization cell migration lamellipodium assembly positive regulation of actin filament polymerization negative regulation of peptidyl-serine phosphorylation positive regulation of translation in response to endoplasmic reticulum stress positive regulation of T cell proliferation positive regulation of transcription from RNA polymerase II promoter ephrin receptor signaling pathway dendritic spine development positive regulation of endoplasmic reticulum stress-induced intrinsic apoptotic signaling pathway negative regulation of PERK-mediated unfolded protein response negative regulation of endoplasmic reticulum stress-induced eIF2 alpha phosphorylation negative regulation of transcription from RNA polymerase II promoter in response to endoplasmic reticulum stress phosphorylation regulation of endoplasmic reticulum stress-induced eIF2 alpha phosphorylation signal transduction signal complex assembly epidermal growth factor receptor signaling pathway regulation of epidermal growth factor-activated receptor activity negative regulation of cell proliferation vesicle-mediated transport T cell activation positive regulation of GTPase activity vascular endothelial growth factor receptor signaling pathway
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