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FIGURE: 1 / 1
NCS1 Antibody (MA5-44903) in WB
Western blot analysis of NCS1 in different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. Samples were incubated in NCS1 Monoclonal antibody (Product # MA5-44903) using a dilution of 1:500 in 5% BSA at room temperature for 2 hours followed by Goat Anti-Rabbit IgG - HRP secondary antibody at a dilution of 1:5,000 for 1 hour at room temperature. Positive control: Lane 1: SH-SY5Y cell lysate; Lane 2: Rat brain tissue lysate; Lane 3: Mouse cerebellum tissue lysate.
Product Details
MA5-44903
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Expression System
HEK293 cells
Class
Recombinant Monoclonal
Type
Antibody
Clone
JE56-39
Immunogen
Recombinant protein within human NCS1 aa 90-190.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Protein A
Storage buffer
TBS, pH 7.4, with 40% glycerol, 0.05% BSA
Contains
0.05% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2931359
Target Information
This gene is a member of the neuronal calcium sensor gene family, which encode calcium-binding proteins expressed predominantly in neurons. The protein encoded by this gene regulates G protein-coupled receptor phosphorylation in a calcium-dependent manner and can substitute for calmodulin. The protein is associated with secretory granules and modulates synaptic transmission and synaptic plasticity. Multiple transcript variants encoding different isoforms have been found for this gene.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: frequenin; Frequenin homolog; Frequenin-like protein; Frequenin-like ubiquitous protein; NCS-1; Neuronal calcium sensor 1; neuronal calcium sensor-1
Gene Aliases: 9430075O15Rik; A730032G13Rik; AI836659; FLUP; FREQ; Mfreq; NCS-1; NCS1
UniProt ID: (Human) P62166, (Mouse) Q8BNY6, (Rat) P62168
Entrez Gene ID: (Human) 23413, (Mouse) 14299, (Rat) 65153
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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