Invitrogen

NCoR1 Polyclonal Antibody

NCoR1 Antibody in Immunocytochemistry (ICC/IF)

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NCoR1 Antibody (PA1-844A) in ICC/IF

Immunofluorescent analysis of NCoR (green) showing staining in the nucleus and cytoplasm of MCF-7 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with a NCoR polyclonal antibody (Product # PA1-844A) in 3% BSA-PBS at a dilution of 1:200 and incubated overnight at 4 ºC in a humidified chamber. Cells were washed with PBST and incubated with a DyLight-conjugated secondary antibody in PBS at room temperature in the dar... View More

NCoR1 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

NCoR1 Antibody in Western Blot, Immunohistochemistry (WB, IHC)

Product Image Gallery

NCoR1 Antibody — Fig. 2 Characterization of adipocyte nuclear corepressor-knockout (ap2-NCoRflx/flx; AKO) mice. Skin, serum, lung, liver, and adipose tissues were harvested from untreated AKO and littermate control mice on a high-fat diet. Tissues were assessed for histologic and biochemical changes suggestive of alterations in adipocyte function. a Nuclear corepressor (NCoR) expression is significantly reduced in adipose tissue but not in lung, liver, or skin. Tissue was harvested from 3- to 4-week-old mice, and messenger RNA (mRNA) levels were determined by qPCR. The results represent fold changes in triplicate determinations from three or four mice per group and normalized to GAPDH. ** p < 0.01 by Mann-Whitney U test. Black bars, wild-type (WT) mice; white bars, AKO mice. b NCoR IHC staining of perirenal adipose tissue evident in WT mice was not detectible in AKO mice. Arrowheads indicate nuclei with positive staining for NCoR. Representative images are shown. Scale bars = 100 µm. c Quantification of NCoR+ cells in perirenal and intradermal adipose tissue (cells per high-power field [HPF]). The results are derived from 5 HPFs from two or three mice per group. * p < 0.05 by Mann-Whitney U test. d AKO mice have reduced expansion of adipocyte size and increased adipocyte number. Representative images of H&E staining of perirenal adipose tissue from mice fed a high-fat diet. Scale bars = 100 µm. e Quantification of adipocyte size (left) and number (right) in perirenal (top) and intradermal (bottom) adipocytes. Adipocyte diameter and number (area results derived from > 200 adipocy {KO}

Product Details

PA1-844A

Applications

Tested Dilution

Publications

Western Blot (WB)

1 µg/mL

View 10 publications 10 publications

Immunohistochemistry (IHC)

View 4 publications 4 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:200-1:1,000

View 1 publication 1 publication

Immunocytochemistry (ICC/IF)

1:100-1:500

View 1 publication 1 publication

ELISA (ELISA)

View 1 publication 1 publication

Immunoprecipitation (IP)

Assay-dependent

ChIP assay (ChIP)

Assay-dependent

View 9 publications 9 publications

Product Specifications

Species Reactivity

Human, Mouse

Published species

Human, Mouse, Non-human primate, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Synthetic peptide corresponding to residues C P(2427) A P L L S A Q Y E T L S D S D D(2443) of mouse N-CoR.

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.7 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS with 1mg/mL BSA

Contains

0.05% sodium azide

Storage conditions

-20°C, Avoid Freeze/Thaw Cycles

Shipping conditions

Ambient (domestic); Wet ice (international)

AB_2149004

Product Specific Information

PA1-844A detects human and mouse nuclear receptor corepressor (N-CoR).

PA1-844A has been successfully used in Western blot, ICC/IF, IHC (P) and immunoprecipitation (ChIP) procedures. By Western blot, this antibody detects a protein at ~270 kDa representing N-CoR from HeLa cell extracts and mouse RAW 264.7/primary macrophages. This antibody is not recommended for mouse C2C12 cells in immunofluorescent applications.

The PA1-844A immunogen is a synthetic peptide corresponding to residues C P(2427) A P L L S A Q Y E T L S D S D D(2443) of mouse N-CoR. This immunizing peptide (Cat. # PEP-061) is available for use in neutralization and control experiments.

Target Information

This gene encodes a protein that mediates ligand-independent transcription repression of thyroid-hormone and retinoic-acid receptors by promoting chromatin condensation and preventing access of the transcription machinery. It is part of a complex which also includes histone deacetylases and transcriptional regulators similar to the yeast protein Sin3p. This gene is located between the Charcot-Marie-Tooth and Smith-Magenis syndrome critical regions on chromosome 17. Alternate splicing results in multiple transcript variants. Pseudogenes of this gene are found on chromosomes 17 and 20.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Chaperone-mediated autophagy dysregulation during aging impairs hepatic fatty acid oxidation via accumulation of NCoR1.

Molecular metabolism

Choi YJ,Yun SH,Yu J,Mun Y,Lee W,Park CJ,Han BW,Lee BH

Published figure using NCoR1 polyclonal antibody (Product # PA1-844A) in Immunohistochemistry (Paraffin)

Sun Oct 01 00:00:00 UTC 2023

NF-κB Transcriptional Activity Indispensably Mediates Hypoxia-Reoxygenation Stress-Induced microRNA-210 Expression.

International journal of molecular sciences

Marwarha G,Slagsvold KH,Høydal MA

PA1-844A was used in ELISA to investigate the molecular mechanisms underlying the induction of microRNA-210 (miR-210) during cellular reoxygenation after hypoxia-reoxygenation (H-R) stress, revealing that the transcription factor NF-κB plays an essential role in mediating H-R-induced miR-210 expression through direct transcriptional up-regulation and reorchestration of histone modifications.

Sat Apr 01 00:00:00 UTC 2023

YAP/TAZ deficiency reprograms macrophage phenotype and improves infarct healing and cardiac function after myocardial infarction.

PLoS biology

Mia MM,Cibi DM,Abdul Ghani SAB,Song W,Tee N,Ghosh S,Mao J,Olson EN,Singh MK

PA1-844A was used in Western Blotting to demonstrate that expression of YAP and TAZ is increased in macrophages undergoing pro-inflammatory or reparative phenotype changes.

Tue Dec 01 00:00:00 UTC 2020

A cell type-specific expression map of NCoR1 and SMRT transcriptional co-repressors in the mouse brain.

The Journal of comparative neurology

Iemolo A,Montilla-Perez P,Lai IC,Meng Y,Nolan S,Wen J,Rusu I,Dulcis D,Telese F

PA1-844A was used in Western Blot, Immunohistochemistry to map gene expression patterns throughout the adult mouse brain.

Tue Sep 01 00:00:00 UTC 2020

A cell type-specific expression map of NCoR1 and SMRT transcriptional co-repressors in the mouse brain.

The Journal of comparative neurology

Iemolo A,Montilla-Perez P,Lai IC,Meng Y,Nolan S,Wen J,Rusu I,Dulcis D,Telese F

PA1-844A was used in Western Blot, Immunohistochemistry to map gene expression patterns throughout the adult mouse brain.

Tue Sep 01 00:00:00 UTC 2020

PPARβ/δ recruits NCOR and regulates transcription reinitiation of ANGPTL4.

Nucleic acids research

Legrand N,Bretscher CL,Zielke S,Wilke B,Daude M,Fritz B,Diederich WE,Adhikary T

PA1-844A was used in Western Blotting to corroborate that deacetylase-dependent and -independent repressive functions of HDAC3- containing complexes which act in parallel to downregulate transcription.

Thu Oct 10 00:00:00 UTC 2019

Adipocyte-specific Repression of PPAR-gamma by NCoR Contributes to Scleroderma Skin Fibrosis.

Arthritis research & therapy

Korman B,Marangoni RG,Lord G,Olefsky J,Tourtellotte W,Varga J

PA1-844A was used in Immunohistochemistry to study the impact of dysregulated peroxisome proliferator activated receptor-γ in systemic sclerosis skin fibrosis.

Wed Jul 11 00:00:00 UTC 2018

Glucocorticoid Receptor:MegaTrans Switching Mediates the Repression of an ERα-Regulated Transcriptional Program.

Molecular cell

Yang F,Ma Q,Liu Z,Li W,Tan Y,Jin C,Ma W,Hu Y,Shen J,Ohgi KA,Telese F,Liu W,Rosenfeld MG

PA1-844A was used in Chromatin immunoprecipitation to study the molecular mechanisms underlying the opposing functions of glucocorticoid receptors and oestrogen receptor α in breast cancer development.

Thu May 04 00:00:00 UTC 2017

The corepressor NCOR1 regulates the survival of single-positive thymocytes.

Scientific reports

Müller L,Hainberger D,Stolz V,Hamminger P,Hassan H,Preglej T,Boucheron N,Sakaguchi S,Wiegers GJ,Villunger A,Auwerx J,Ellmeier W

PA1-844A was used in Western Blotting to identify NCOR1 as a crucial regulator of the survival of single-positive thymocytes and reveal its role in the proper generation of the peripheral T cell pool.

Tue Nov 21 00:00:00 UTC 2017

COPS5 amplification and overexpression confers tamoxifen-resistance in ERα-positive breast cancer by degradation of NCoR.

Nature communications

Lu R,Hu X,Zhou J,Sun J,Zhu AZ,Xu X,Zheng H,Gao X,Wang X,Jin H,Zhu P,Guo L

PA1-844A was used in immunohistochemistry - paraffin section and western blot to analyze tamoxifen-resistance in ERalpha-positive breast cancer by degradation of NCoR due to COPS5 amplification and overexpression

Mon Jul 04 00:00:00 UTC 2016

COPS5 amplification and overexpression confers tamoxifen-resistance in ERα-positive breast cancer by degradation of NCoR.

Nature communications

Lu R,Hu X,Zhou J,Sun J,Zhu AZ,Xu X,Zheng H,Gao X,Wang X,Jin H,Zhu P,Guo L

Mon Jul 04 00:00:00 UTC 2016

Energy homeostasis targets chromosomal reconfiguration of the human GH1 locus.

The Journal of clinical investigation

Vakili H,Jin Y,Cattini PA

PA1-844A was used in ChIP assay to study chromosomal reconfiguration of the human GH1 locus by energy homeostasis

Sat Nov 01 00:00:00 UTC 2014

Acute hypoxia affects P-TEFb through HDAC3 and HEXIM1-dependent mechanism to promote gene-specific transcriptional repression.

Nucleic acids research

Safronova OS,Nakahama K,Morita I

PA1-844A was used in immunocytochemistry to study the inhibitory effect of hypoxia on gene transcription and its mechanism

Fri Aug 01 00:00:00 UTC 2014

CtBP2 modulates the androgen receptor to promote prostate cancer progression.

Cancer research

Takayama K,Suzuki T,Fujimura T,Urano T,Takahashi S,Homma Y,Inoue S

PA1-844A was used in western blot to correlate CTBP2 SNPs with prostate cancer development and AR-enhancer activity

Sat Nov 15 00:00:00 UTC 2014

Developmentally regulated GTP-binding protein 2 ameliorates EAE by suppressing the development of TH17 cells.

Clinical immunology (Orlando, Fla.)

Ko MS,Kim HJ,Kim HK,Yoon NA,Lee UH,Lee SC,Chung DK,Lee BJ,Suh JH,Cho WJ,Park JW

PA1-844A was used in ChIP assay to study the role of suppressed Th17 cell development in the mechanism by which transgenic DRG2 overexpression protects against experimental autoimmune encephalomyelitis

Sat Feb 01 00:00:00 UTC 2014

Activity-dependent phosphorylation of MeCP2 threonine 308 regulates interaction with NCoR.

Nature

Ebert DH,Gabel HW,Robinson ND,Kastan NR,Hu LS,Cohen S,Navarro AJ,Lyst MJ,Ekiert R,Bird AP,Greenberg ME

PA1-844A was used in western blot to study an activity-dependent phosphorylation site on MeCP2 that modulates interaction with NCoR

Thu Jul 18 00:00:00 UTC 2013

mTORC1 controls fasting-induced ketogenesis and its modulation by ageing.

Nature

Sengupta S,Peterson TR,Laplante M,Oh S,Sabatini DM

PA1-844A was used in immunohistochemistry to study the role of mTORC1 in ketogenesis and its age-dependent effect

Thu Dec 23 00:00:00 UTC 2010

Proteasomal degradation of retinoid X receptor alpha reprograms transcriptional activity of PPARgamma in obese mice and humans.

The Journal of clinical investigation

Lefebvre B,Benomar Y,Guédin A,Langlois A,Hennuyer N,Dumont J,Bouchaert E,Dacquet C,Pénicaud L,Casteilla L,Pattou F,Ktorza A,Staels B,Lefebvre P

PA1-844A was used in ChIP assay to investigate the RXR alpha gene in obese mice and humans and its effect on transcriptional activity of PPAR gamma

Sat May 01 00:00:00 UTC 2010

Vitamin D receptor binds to the ε germline gene promoter and exhibits transrepressive activity.

The Journal of allergy and clinical immunology

Milovanovic M,Heine G,Hallatschek W,Opitz B,Radbruch A,Worm M

PA1-844A was used in ChIP assay to investigate the mechanism of the inhibition of IgE production mediated by calcitriol

Mon Nov 01 00:00:00 UTC 2010

Control of inducible gene expression by signal-dependent transcriptional elongation.

Cell

Hargreaves DC,Horng T,Medzhitov R

Published figure using NCoR1 polyclonal antibody (Product # PA1-844A) in ChIP assay

Fri Jul 10 00:00:00 UTC 2009

Phosphorylation of liver X receptor alpha selectively regulates target gene expression in macrophages.

Molecular and cellular biology

Torra IP,Ismaili N,Feig JE,Xu CF,Cavasotto C,Pancratov R,Rogatsky I,Neubert TA,Fisher EA,Garabedian MJ

PA1-844A was used in western blot to investigate the function of liver X receptor alpha phosphorylation in macrophages.

Tue Apr 01 00:00:00 UTC 2008

Phosphorylation of liver X receptor alpha selectively regulates target gene expression in macrophages.

Molecular and cellular biology

Torra IP,Ismaili N,Feig JE,Xu CF,Cavasotto C,Pancratov R,Rogatsky I,Neubert TA,Fisher EA,Garabedian MJ

PA1-844A was used in western blot to investigate the function of liver X receptor alpha phosphorylation in macrophages.

Tue Apr 01 00:00:00 UTC 2008

Electromagnetic fields alter the expression of estrogen receptor cofactors in breast cancer cells.

Bioelectromagnetics

Girgert R,Gründker C,Emons G,Hanf V

PA1-844A was used in western blot to investigate the effect of electromagnetic fields on expression of estrogen receptor cofactors in MCF-7 cells

Tue Apr 01 00:00:00 UTC 2008

Nuclear receptor corepressor is a novel regulator of phosphatidylinositol 3-kinase signaling.

Molecular and cellular biology

Furuya F,Guigon CJ,Zhao L,Lu C,Hanover JA,Cheng SY

PA1-844A was used in western blot to indicate that NCoR is a novel regulator of PI3K signaling and could modulate thyroid tumor progression

Sat Sep 01 00:00:00 UTC 2007

Nuclear receptor corepressor is a novel regulator of phosphatidylinositol 3-kinase signaling.

Molecular and cellular biology

Furuya F,Guigon CJ,Zhao L,Lu C,Hanover JA,Cheng SY

PA1-844A was used in western blot to indicate that NCoR is a novel regulator of PI3K signaling and could modulate thyroid tumor progression

Sat Sep 01 00:00:00 UTC 2007

Nuclear receptor corepressor is a novel regulator of phosphatidylinositol 3-kinase signaling.

Molecular and cellular biology

Furuya F,Guigon CJ,Zhao L,Lu C,Hanover JA,Cheng SY

PA1-844A was used in western blot to indicate that NCoR is a novel regulator of PI3K signaling and could modulate thyroid tumor progression

Sat Sep 01 00:00:00 UTC 2007

Thyroid hormone-mediated negative transcriptional regulation of Necdin expression.

Journal of molecular endocrinology

Nygård M,Becker N,Demeneix B,Pettersson K,Bondesson M

PA1-844A was used in ChIP assay to investigate the effect of thyroid hormone on Necdin expression

Thu Jun 01 00:00:00 UTC 2006

A SUMOylation-dependent pathway mediates transrepression of inflammatory response genes by PPAR-gamma.

Nature

Pascual G,Fong AL,Ogawa S,Gamliel A,Li AC,Perissi V,Rose DW,Willson TM,Rosenfeld MG,Glass CK

PA1-844A was used in chromatin immunoprecipitation to study the regulatory mechanism of PPAR gamma in adipogenesis and glucose homeostasis.

Thu Sep 29 00:00:00 UTC 2005

PPARgamma regulates adipocyte cholesterol metabolism via oxidized LDL receptor 1.

The Journal of clinical investigation

Chui PC,Guan HP,Lehrke M,Lazar MA

PA1-844A was used in chromatin immunoprecipitation to investigate the role of the PPARgamma during the adipocyte cholesterol metabolism.

Mon Aug 01 00:00:00 UTC 2005

Bioinformatics

Protein Aliases: h KIAA1047; hCIT529I10; MGC104216; N-CoR; N-CoR1; nuclear receptor corepressor; Nuclear receptor corepressor 1; protein phosphatase 1, regulatory subunit 109; retinoid X receptor interacting protein 13; retinoid X receptor-interacting protein 13; thyroid hormone- and retinoic acid receptor-associated corepressor 1

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Gene Aliases: 5730405M06Rik; A230020K14Rik; hN-CoR; KIAA1047; mKIAA1047; N-CoR; N-CoR1; NCOR1; PPP1R109; RIP13; Rxrip13; TRAC1

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UniProt ID: (Human) O75376

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Entrez Gene ID: (Human) 9611, (Mouse) 20185

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Function(s)

RNA polymerase II regulatory region DNA binding RNA polymerase II activating transcription factor binding chromatin binding transcription corepressor activity protein binding ligand-dependent nuclear receptor binding protein domain specific binding estrogen receptor binding nuclear hormone receptor binding histone deacetylase binding peroxisome proliferator activated receptor binding transcription regulatory region DNA binding retinoid X receptor binding thyroid hormone receptor binding DNA binding transcription factor activity, sequence-specific DNA binding histone deacetylase regulator activity retinoic acid receptor binding sequence-specific DNA binding

Process(es)

negative regulation of transcription from RNA polymerase II promoter CD4-positive, CD25-positive, alpha-beta regulatory T cell differentiation transcription, DNA-templated regulation of transcription, DNA-templated regulation of transcription from RNA polymerase II promoter positive regulation of cell proliferation negative regulation of gene expression negative regulation of phosphatidylinositol 3-kinase signaling chromatin modification thalamus development regulation of histone deacetylation positive regulation of histone deacetylation circadian regulation of gene expression regulation of multicellular organism growth cholesterol homeostasis negative regulation of transcription, DNA-templated negative regulation of JNK cascade regulation of catalytic activity spindle assembly definitive erythrocyte differentiation regulation of glycolytic process by negative regulation of transcription from RNA polymerase II promoter regulation of lipid transport by negative regulation of transcription from RNA polymerase II promoter transcription from RNA polymerase II promoter skeletal muscle tissue development lactation circadian rhythm covalent chromatin modification cerebellum development cellular lipid metabolic process negative regulation of production of miRNAs involved in gene silencing by miRNA regulation of fatty acid transport

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If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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NCoR1 Polyclonal Antibody

NCoR1 Antibody (PA1-844A) in ICC/IF

Product Details

PA1-844A

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (Paraffin) (IHC (P))

Immunocytochemistry (ICC/IF)

ELISA (ELISA)

Immunoprecipitation (IP)

ChIP assay (ChIP)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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