Invitrogen

NCoR2 Polyclonal Antibody

NCoR2 Antibody in Immunocytochemistry (ICC/IF)

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NCoR2 Antibody (PA1-843) in ICC/IF

Immunofluorescent analysis of NCoR2 (green) showing staining in the nucleus of Neuro-2a cells (right) compared to a negative control without primary antibody (left). Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with a NCoR2 polyclonal antibody (Product # PA1-843) in 3% BSA-PBS at a dilution of 1:150 and incubated overnight at 4 ºC in a humidified chamber. Cells were washed with PBST and incubated with a DyLight-conjug... View More

NCoR2 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

NCoR2 Antibody in Immunohistochemistry (IHC)

NCoR2 Antibody in Western Blot, Immunoprecipitation (WB, IP)

Product Image Gallery

NCoR2 Antibody — SMRT and FOXP1 coregulate c-fms expression in THP-1 cells. (A) Effect of siRNAs specific to FOXP1 or SMRT on c-fms mRNA levels as measured by qRT-PCR (±SEM; [*] P < 0.01, [**] P < 0.005). (B,C) qPCR analysis of chromatin-immunoprecipitated SMRT or FOXP1 on untreated (undiff) and PMA-treated (diff) THP-1 cells using specific anti-SMRT and anti-FOXP1 antibodies and oligonucleotides surrounding the FOX-binding site of the c-fms promoter. (D) Agarose gel representation of ChIP analysis in B and C. (E,F) qPCR analysis of chromatin-immunoprecipitated SMRT or FOXP1 using oligonucleotides surrounding the FOX-binding site of the c-fms promoter performed on THP-1 cells lipofected with FOXP1 siRNA. {KD}

Product Details

PA1-843

Applications

Tested Dilution

Publications

Western Blot (WB)

4 µg/mL

View 7 publications 7 publications

Immunohistochemistry (IHC)

View 1 publication 1 publication

Immunohistochemistry (Paraffin) (IHC (P))

1:100-1:1,000

Immunocytochemistry (ICC/IF)

1:100-1:200

Immunoprecipitation (IP)

View 2 publications 2 publications

ChIP assay (ChIP)

View 4 publications 4 publications

Product Specifications

Species Reactivity

Human, Mouse

Published species

Human, Mouse

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

His-tagged fusion protein corresponding to amino acid residues 95-565 from human SMRT.

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS with 1mg/mL BSA

Contains

0.05% sodium azide

Storage conditions

-20°C, Avoid Freeze/Thaw Cycles

Shipping conditions

Ambient (domestic); Wet ice (international)

AB_2149135

Product Specific Information

PA1-843 detects silencing mediator of retinoic acid and thyroid hormone receptor (SMRT) from human and mouse cells.

PA1-843 has been successfully used in Western blot, ICC/IF and immunohistochemistry (paraffin) procedures. By Western blot, this antibody detects an ~250 kDa protein representing SMRT from HeLa cell nuclear extracts.

The PA1-843 antigen is His-tagged fusion protein corresponding to amino acid residues 95-565 from human SMRT.

Target Information

Steroid and thyroid hormones and retinoic acid regulate a complex array of gene expression activity via intracellular receptor transcription factors belonging to the ligand-dependent nuclear receptor superfamily. Adding to the complexity of function of these transcription factors are associated proteins known as coactivators and corepressors which, as their names suggest, enhance or depress transcription activity of the nuclear receptor with which they associate. Silencing mediator of retinoic acid and thyroid hormone receptor (SMRT) and nuclear receptor corepressor (N-CoR) are related transcriptional corepressors which contain two distinct domains capable of interacting with unliganded nuclear receptors to repress their basal transcriptional activity.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

CRISPR/Cas9 genome editing reveals an essential role for basigin in maintaining a nonkeratinized squamous epithelium in cornea.

FASEB bioAdvances

Woodward AM,Feeley MN,Rinaldi J,Argüeso P

Published figure using NCoR2 polyclonal antibody (Product # PA1-843) in Western Blot

Mon Nov 01 00:00:00 UTC 2021

TBL1XR1 Mutations Drive Extranodal Lymphoma by Inducing a Pro-tumorigenic Memory Fate.

Cell

Venturutti L,Teater M,Zhai A,Chadburn A,Babiker L,Kim D,Béguelin W,Lee TC,Kim Y,Chin CR,Yewdell WT,Raught B,Phillip JM,Jiang Y,Staudt LM,Green MR,Chaudhuri J,Elemento O,Farinha P,Weng AP,Nissen MD,Steidl C,Morin RD,Scott DW,Privé GG,Melnick AM

PA1-843 was used in Immunoprecipitation to show that TBL1XR1 mutations skew the humoral immune response toward generating abnormal immature memory B cells (MB), while impairing plasma cell differentiation.

Thu Jul 23 00:00:00 UTC 2020

ARNT2 Tunes Activity-Dependent Gene Expression through NCoR2-Mediated Repression and NPAS4-Mediated Activation.

Neuron

Sharma N,Pollina EA,Nagy MA,Yap EL,DiBiase FA,Hrvatin S,Hu L,Lin C,Greenberg ME

PA1-843 was used in Western Blotting to conclude that the interplay of bHLH-PAS complexes at activity-dependent regulatory elements maintains temporal control of activity-dependent gene expression and scales somatic inhibition with circuit activity.

Wed Apr 17 00:00:00 UTC 2019

Autoregulatory loop of nuclear corepressor 1 expression controls invasion, tumor growth, and metastasis.

Proceedings of the National Academy of Sciences of the United States of America

Martínez-Iglesias OA,Alonso-Merino E,Gómez-Rey S,Velasco-Martín JP,Martín Orozco R,Luengo E,García Martín R,Ibáñez de Cáceres I,Fernández AF,Fraga MF,González-Peramato P,Varona C,Palacios J,Regadera J,Aranda A

PA1-843 was used in western blot to study how nuclear corepressor 1 contributes to cancer

Tue Jan 19 00:00:00 UTC 2016

PHD2/3-dependent hydroxylation tunes cardiac response to β-adrenergic stress via phospholamban.

The Journal of clinical investigation

Xie L,Pi X,Townley-Tilson WH,Li N,Wehrens XH,Entman ML,Taffet GE,Mishra A,Peng J,Schisler JC,Meissner G,Patterson C

PA1-843 was used in immunoprecipitation to investigate how PHD2 and PHD3 modulate contractile function.

Wed Jul 01 00:00:00 UTC 2015

PHD2/3-dependent hydroxylation tunes cardiac response to β-adrenergic stress via phospholamban.

The Journal of clinical investigation

Xie L,Pi X,Townley-Tilson WH,Li N,Wehrens XH,Entman ML,Taffet GE,Mishra A,Peng J,Schisler JC,Meissner G,Patterson C

PA1-843 was used in immunoprecipitation to investigate how PHD2 and PHD3 modulate contractile function.

Wed Jul 01 00:00:00 UTC 2015

NCoR1 and SMRT play unique roles in thyroid hormone action in vivo.

Molecular and cellular biology

Shimizu H,Astapova I,Ye F,Bilban M,Cohen RN,Hollenberg AN

PA1-843 was used in western blot to examine the role of coregulators of nuclear receptor action in thyroid hormone action

Sun Feb 01 00:00:00 UTC 2015

NCoR1 and SMRT play unique roles in thyroid hormone action in vivo.

Molecular and cellular biology

Shimizu H,Astapova I,Ye F,Bilban M,Cohen RN,Hollenberg AN

PA1-843 was used in western blot to examine the role of coregulators of nuclear receptor action in thyroid hormone action

Sun Feb 01 00:00:00 UTC 2015

Thyroid hormone signaling in vivo requires a balance between coactivators and corepressors.

Molecular and cellular biology

Vella KR,Ramadoss P,Costa-E-Sousa RH,Astapova I,Ye FD,Holtz KA,Harris JC,Hollenberg AN

PA1-843 was used in western blot to study the role of the coactivator/corepressor balance in thyroid hormone signaling

Thu May 01 00:00:00 UTC 2014

Corepressors (NCoR and SMRT) as well as coactivators are recruited to positively regulated 1α,25-dihydroxyvitamin D3-responsive genes.

The Journal of steroid biochemistry and molecular biology

Meyer MB,Pike JW

PA1-843 was used in ChIP assay to study the recruitment of both co-activators and co-repressors to vitamin D3-responsive genes

Mon Jul 01 00:00:00 UTC 2013

A FOXO3-IRF7 gene regulatory circuit limits inflammatory sequelae of antiviral responses.

Nature

Litvak V,Ratushny AV,Lampano AE,Schmitz F,Huang AC,Raman A,Rust AG,Bergthaler A,Aitchison JD,Aderem A

PA1-843 was used in ChIP assay to study the role of FOXO3 and IRF7 in minimising inflammatory damage in response to viral infection

Thu Oct 18 00:00:00 UTC 2012

A downstream intergenic cluster of regulatory enhancers contributes to the induction of CYP24A1 expression by 1alpha,25-dihydroxyvitamin D3.

The Journal of biological chemistry

Meyer MB,Goetsch PD,Pike JW

Published figure using NCoR2 polyclonal antibody (Product # PA1-843) in ChIP assay

Fri May 14 00:00:00 UTC 2010

Cooperative regulation in development by SMRT and FOXP1.

Genes & development

Jepsen K,Gleiberman AS,Shi C,Simon DI,Rosenfeld MG

PA1-843 was used in chromatin immunoprecipitation to study the roles of SMRT and FOXP1 in developmental regulation.

Sat Mar 15 00:00:00 UTC 2008

Cooperative regulation in development by SMRT and FOXP1.

Genes & development

Jepsen K,Gleiberman AS,Shi C,Simon DI,Rosenfeld MG

PA1-843 was used in chromatin immunoprecipitation to study the roles of SMRT and FOXP1 in developmental regulation.

Sat Mar 15 00:00:00 UTC 2008

Ataxin 1, a SCA1 neurodegenerative disorder protein, is functionally linked to the silencing mediator of retinoid and thyroid hormone receptors.

Proceedings of the National Academy of Sciences of the United States of America

Tsai CC,Kao HY,Mitzutani A,Banayo E,Rajan H,McKeown M,Evans RM

PA1-843 was used in western blot and immunocytochemistry to demonstrate the effect of mutant Atx1 on corepressor-dependent transcriptional pathways.

Tue Mar 23 00:00:00 UTC 2004

Bioinformatics

Protein Aliases: CTG repeat protein 26; N-CoR2; nuclear receptor corepressor; Nuclear receptor corepressor 2; silencing mediator for retinoid and thyroid hormone receptors; Silencing mediator of retinoic acid and thyroid hormone receptor; silencing mediator of retinoid acid and thyroid hormone receptor; SMAP270; SMRT; T3 receptor-associating factor; Thyroid-, retinoic-acid-receptor-associated corepressor; TRAC

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Gene Aliases: CTG26; N-CoR; N-CoR2; NCOR2; SMAP270; SMRT; SMRTE; SMRTE-tau; TNRC14; TRAC; TRAC-1; TRAC1

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UniProt ID: (Human) Q9Y618, (Mouse) Q9WU42

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Entrez Gene ID: (Human) 9612, (Mouse) 20602

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Function(s)

DNA binding transcription corepressor activity Notch binding protein binding transcription factor binding ligand-dependent nuclear receptor binding histone deacetylase binding protein N-terminus binding RNA polymerase II regulatory region DNA binding chromatin binding protein complex binding glucocorticoid receptor binding retinoic acid receptor binding sequence-specific DNA binding retinoid X receptor binding

Process(es)

negative regulation of transcription from RNA polymerase II promoter in utero embryonic development heart morphogenesis transcription, DNA-templated regulation of transcription, DNA-templated telencephalon development cell proliferation in forebrain forebrain development glucose homeostasis negative regulation of fat cell differentiation negative regulation of transcription, DNA-templated white fat cell differentiation Type I pneumocyte differentiation regulation of cellular ketone metabolic process by negative regulation of transcription from RNA polymerase II promoter positive regulation of protein deacetylation cellular lipid metabolic process negative regulation of production of miRNAs involved in gene silencing by miRNA

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NCoR2 Polyclonal Antibody

NCoR2 Antibody (PA1-843) in ICC/IF

Product Details

PA1-843

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (Paraffin) (IHC (P))

Immunocytochemistry (ICC/IF)

Immunoprecipitation (IP)

ChIP assay (ChIP)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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