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NDFIP2 Antibody (MA5-49299) in ICC/IF
Immunocytochemistry analysis of NDFIP2 in A431 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent for 15 mins. The cells were blocked with 10% goat serum. Samples were then incubated in NDFIP2 Monoclonal antibody (Product # MA5-49299) using a dilution of 5 μg/mL. DyLight®594 Conjugated Goat Anti-Mouse IgG was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate f... View More
Product Details
MA5-49299
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Mouse
/ IgG2b
Class
Monoclonal
Type
Antibody
Clone
10D6D7
Immunogen
E.coli-derived human NDFIP2 recombinant protein (Position: M16-L336).
Conjugate
Form
Lyophilized
Concentration
500 µg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS with 4mg trehalose
Contains
no preservative
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_3074957
Product Specific Information
Adding 0.2 mL of distilled water will yield a concentration of 500 µg/mL.
Positive Control - WB: human A549 whole cell, human HepG2 whole cell, human MCF-7 whole cell, human Hela whole cell. IHC: human placenta tissue, human squamous cell lung carcinoma tissue, human breast cancer tissue, human spleen tissue. ICC/IF: A431 cell. Flow: JK cell.|Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.
Target Information
Activates HECT domain-containing E3 ubiquitin-protein ligases, including ITCH, NEDD4, NEDD4L, SMURF2, WWP1 and WWP2, and consequently modulates the stability of their targets. As a result, may control many cellular processes. Recruits ITCH, NEDD4 and SMURF2 to endosomal membranes. Negatively regulates KCNH2 potassium channel activity by decreasing its cell-surface expression and interfering with channel maturation through recruitment of NEDD4L to the Golgi apparatus and multivesicular body where it mediates KCNH2 degradation (PubMed:26363003). May modulate EGFR signaling. Together with NDFIP1, limits the cytokine signaling and expansion of effector Th2 T-cells by promoting degradation of JAK1, probably by ITCH- and NEDD4L-mediated ubiquitination. [UniProt]
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: MAPK-activating protein PM04 PM05 PM06 PM07; NEDD4 family-interacting protein 2; NEDD4 WW domain-binding protein 5A; NF-kappa-B-activating protein 413; Putative MAPK-activating protein PM04/PM05/PM06/PM07; Putative NF-kappa-B-activating protein 413
Gene Aliases: KIAA1165; N4WBP5A; NDFIP2
UniProt ID: (Human) Q9NV92
Entrez Gene ID: (Human) 54602
Performance Guarantee
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