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Invitrogen
NDUFA1 Polyclonal Antibody
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FIGURE: 1 / 13
NDUFA1 Antibody (PA5-79724) in ICC/IF
Immunocytochemistry/Immunofluorescence analysis of NDUFA1 in Hela cells using NDUFA1 Polyclonal Antibody (Product # PA5-79724). Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent for 15 mins. The cells were blocked with 10% goat serum and incubated with the primary antibody at 5 µg/mL. Cy3 conjugated goat anti-rabbit IgG was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appr... View More
Product Details
PA5-79724
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
A synthetic peptide corresponding to a sequence at the C-terminus of human NDUFA1 (54-70aa ISGVDRYYVSKGLENID).
Conjugate
Form
Lyophilized
Concentration
500 µg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS with 5mg BSA
Contains
0.05mg sodium azide, 0.05mg thimerosal
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2746839
Product Specific Information
Reconstitute with 0.2 mL of distilled water to yield a concentration of 500 µg/mL.
Positive Control - WB: human 293T whole cell, rat heart tissue, rat kidney tissue, mouse heart tissue, mouse kidney tissue. IHC: human skeletal muscle tissue, mouse heart tissue, rat heart tissue IHC-F: Rat Cardiac Muscle tissue. ICC/IF: Hela cell. Flow: HepG2 cell.
Target Information
Accessory subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I), that is believed not to be involved in catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone.
⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: CI-MWFE; complex I MWFE subunit; Complex I-MWFE; NADH dehydrogenase (ubiquinone) 1 alpha subcomplex, 1 (7.5kD, MWFE); NADH dehydrogenase (ubiquinone) 1 alpha subcomplex, 1, 7.5kDa; NADH dehydrogenase 1 alpha subcomplex, 1; NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 1; NADH oxidoreductase subunit MWFE; NADH ubiquinone oxidoreductase subunit MWFE; NADH-ubiquinone oxidoreductase MWFE subunit; NADH:ubiquinone oxidoreductase (complex 1); type I dehydrogenase
Gene Aliases: 1810049F12Rik; CI-MWFE; MWFE; NDUFA1; RGD1560955; ZNF183
UniProt ID: (Human) O15239, (Mouse) O35683
Entrez Gene ID: (Human) 4694, (Mouse) 54405, (Rat) 363441
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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