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FIGURE: 1 / 1
NDUFA1 Antibody (PA5-97007) in WB
Western blot analysis of NDUFA1 in extracts of various cell lines. Samples were incubated with NDUFA1 Polyclonal antibody (Product # PA5-97007) using a dilution of 1:1,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 30s.
Product Details
PA5-97007
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 1-70 of human NDUFA1 (NP_0045321)
Conjugate
Form
Liquid
Concentration
1.09 mg/mL
Purification
Affinity Chromatography
Storage buffer
PBS, pH 7.3, with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Wet ice
RRID
AB_2808809
Product Specific Information
Immunogen sequence: MWFEILPGLS VMGVCLLIPG LATAYIHRFT NGGKEKRVAH FGYHWSLMER DRRISGVDRY YVSKGLENID; Positive Samples: MCF-7, Mouse kidney, Mouse heart, Rat brain; Cellular Location: Matrix side, Mitochondrion inner membrane, Single-pass membrane protein
Target Information
Accessory subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I), that is believed not to be involved in catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: CI-MWFE; complex I MWFE subunit; Complex I-MWFE; NADH dehydrogenase (ubiquinone) 1 alpha subcomplex, 1 (7.5kD, MWFE); NADH dehydrogenase (ubiquinone) 1 alpha subcomplex, 1, 7.5kDa; NADH dehydrogenase 1 alpha subcomplex, 1; NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 1; NADH oxidoreductase subunit MWFE; NADH ubiquinone oxidoreductase subunit MWFE; NADH-ubiquinone oxidoreductase MWFE subunit; NADH:ubiquinone oxidoreductase (complex 1); type I dehydrogenase
Gene Aliases: 1810049F12Rik; CI-MWFE; MWFE; NDUFA1; RGD1560955; ZNF183
UniProt ID: (Human) O15239, (Mouse) O35683
Entrez Gene ID: (Human) 4694, (Mouse) 54405, (Rat) 363441
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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